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重组

重组Anti-PAX9抗体[7C2] (ab28538)

Reviews (1)Q&A (2)References (11)
Western blot - Anti-PAX9 antibody [7C2] (ab28538)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX9 antibody [7C2] (ab28538)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7C2] to PAX9
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-PAX9抗体[7C2]
    参阅全部 PAX9 一抗

  • 描述

    大鼠单克隆抗体[7C2] to PAX9

  • 宿主

    Rat

  • 特异性

    Does not cross react with other PAX proteins

  • 经测试应用

    适用于: ICC/IF, WBmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse esophagus, Mouse skeletal muscle tissue. ICC/IF: NIH/3T3 cells.

  • 常规说明

    This product has switched from a hybridoma to recombinant production method on 08th March 2021.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab28538于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/20.
WB
1/1000. Predicted molecular weight: 38 kDa.
说明
ICC/IF
1/20.
WB
1/1000. Predicted molecular weight: 38 kDa.
应用说明
Is unsuitable for IHC-P.

靶标

  • 功能

    Transcription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs.

  • 疾病相关

    Defects in PAX9 are the cause of tooth agenesis selective type 3 (STHAG3) [MIM:604625]. A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth).

  • 序列相似性

    Contains 1 paired domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Paired box 9 antibody
      • Paired box gene 9 antibody
      • Paired box homeotic gene 9 antibody
      • Paired box protein 9 antibody
      • Paired box protein Pax 9 antibody
      • Paired box protein Pax-9 antibody
      • Paired box protein Pax9 antibody
      • Paired domain gene 9 antibody
      • PAX 9 antibody
      • PAX9 antibody
      • PAX9_HUMAN antibody
      • STHAG3 antibody
      see all

    图片

    • Western blot - Anti-PAX9 antibody [7C2] (ab28538)
      Western blot - Anti-PAX9 antibody [7C2] (ab28538)
      All lanes : Anti-PAX9 antibody [7C2] (ab28538) at 1/1000 dilution

      Lane 1 : Mouse esophagus tissue lysate
      Lane 2 : Mouse skeletal muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

      Predicted band size: 38 kDa


      Exposure time: 70 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST

       

    • Immunocytochemistry/ Immunofluorescence - Anti-PAX9 antibody [7C2] (ab28538)
      Immunocytochemistry/ Immunofluorescence - Anti-PAX9 antibody [7C2] (ab28538)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling PAX9 with ab28538 at 1/20 (49.7 µg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in NIH/3T3 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    文献 (11)

    发表研究结果有使用 ab28538？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab28538 被引用在 11 文献中.

    • Pinzon-Guzman C  et al. Noggin regulates foregut progenitor cell programming, and misexpression leads to esophageal atresia. J Clin Invest 130:4396-4410 (2020). PubMed: 32427591
    • Koterazawa Y  et al. Retinoic acid receptor ? activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium. J Gastroenterol 55:763-774 (2020). PubMed: 32556644
    • Yuan Y  et al. Spatiotemporal cellular movement and fate decisions during first pharyngeal arch morphogenesis. Sci Adv 6:N/A (2020). PubMed: 33328221
    • Phillips HM  et al. Pax9 is required for cardiovascular development and interacts with Tbx1 in the pharyngeal endoderm to control 4th pharyngeal arch artery morphogenesis. Development 146:N/A (2019). PubMed: 31444215
    • Bar C  et al. Polycomb Repressive Complex 1 Controls Maintenance of Fungiform Papillae by Repressing Sonic Hedgehog Expression. Cell Rep 28:257-266.e5 (2019). PubMed: 31269445
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-PAX9 antibody [7C2]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E11.5 embryos)
    Permeabilization
    No
    Specification
    E11.5 embryos
    Blocking step
    PBSMBT (1X PBS, 1.5% Milk, 1.5% BSA, 0.1% Triton X-100) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. William Wood

    Verified customer

    提交于 Apr 30 2014

    Question

    We see no staining for PAX9 from mouse tongue sections, paraffin-embedded or frozen.

    Read More
    Answer

    Here is the IHC protocol I received from the laboratory that validated the antibody for this application. Please let me know if you have any questions. As I mentioned in my previous e-mail, if your protocol is similar to this one, I will be happy to discuss a replacement.

    Serial sections (5 mm) were

    mounted onto poly-L-lysine-coated slides, baked at

    65°C overnight, deparaffinized and dehydrated according

    to standard protocols. For antigen retrieval, specimens

    were boiled for 4 min in a pressure cooker in

    citrate buffer (10 mM, pH 6.0). Non-specific binding of

    the primary antibodies was reduced by pre-incubation

    of the slides with 4% dried skimmed milk in antibody

    diluent. Immunostaining was performed by the streptavidin–

    alkaline phosphatase technique using the Dako

    ChemMate Detection Kit (DAKO, Denmark) according

    to the manufacturer’s instruction, and using Fast

    Red as a colour substrate. Sections were counterstained

    with haematoxylin. Only distinct nuclear

    staining of PAX9 was considered positive and staining

    intensity was not graded in this study. Immunoreactive

    cells were estimated as a percentage of the total number

    of tumour cells and dysplastic cells, respectively.

    PAX9 expression in dysplasias and in invasive carcinomas

    was classified as reduced when the fraction of

    PAX9-positive cells was smaller than 50%, and

    increased when the fraction of PAX9-positive cells

    was greater than 95%.

    Read More

    Question

    Dear Technical Team,




    Hope this e-mail finds you well!




    Kindly let us know the availability of Anti Mouse PAX9 antibody for the applications of both ChIP and Co- IP as enquired by the customer:





    Looking forward towards your valued response on it.





    With Best Regards,

    Read More
    Answer

    Thank you for your enquiry and your interest.

    We have several antibodies against PAX9 but none of them have been tested specifically in ChIP application. Here are some which recognize mouse (murine) PAX9 and detect the native form of the protein so there is a high chance that they work in ChIP as well: ab28538, ab84830, ab85168.

    I could certainly offer a Testing Discount code for your customer if she would liek to test one of them. The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

    Please do let me know if your customer is interested in this offer. I look forward to hearing from you soon.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com