Anti-Patched / PTCH1抗体(ab51983)
Key features and details
- Goat polyclonal to Patched / PTCH1
- Suitable for: ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-Patched / PTCH1抗体
参阅全部 Patched / PTCH1 一抗 -
描述
山羊多克隆抗体to Patched / PTCH1 -
宿主
Goat -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Dog, Pig
-
免疫原
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51983于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
|
|
| WB | (1) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 161 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
ICC
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 161 kDa). 1 hour primary incubation is recommended for this product. |
靶标
-
功能
Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. -
组织特异性
In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin. -
疾病相关
Defects in PTCH1 are probably the cause of basal cell nevus syndrome (BCNS) [MIM:109400]; also known as Gorlin syndrome or Gorlin-Goltz syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas (NBCCS) and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas (BCC), fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 is also mutated in squamous cell carcinoma (SCC). Could also be associated with large body size observed in BCNS patients.
Defects in PTCH1 are a cause of sporadic basal cell carcinoma (BCC) [MIM:605462].
Defects in PTCH1 are the cause of holoprosencephaly type 7 (HPE7) [MIM:610828]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. -
序列相似性
Belongs to the patched family.
Contains 1 SSD (sterol-sensing) domain. -
发展阶段
In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. -
翻译后修饰
Glycosylation is necessary for SHH binding. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 5727 Human
- Entrez Gene: 19206 Mouse
- Omim: 601309 Human
- SwissProt: Q13635 Human
- SwissProt: Q86XG7 Human
- SwissProt: Q61115 Mouse
- Unigene: 494538 Human
- Unigene: 228798 Mouse
-
别名
- A230106A15Rik antibody
- BCNS antibody
- FLJ26746 antibody
see all
图片
-
Immunocytochemistry - Anti-Patched / PTCH1 antibody (ab51983)Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells permeabilized with 0.15% Triton staining Patched / PTCH1. Primary incubation with ab51983 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.
-
Western blot - Anti-Patched / PTCH1 antibody (ab51983)Anti-Patched / PTCH1 antibody (ab51983) at 1 µg/ml + Human Brain lysate in RIPA buffer at 35 µg
Predicted band size: 161 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
实验方案
数据表及文件
-
Datasheet download
文献 (4)
ab51983 被引用在 4 文献中.
- Hu L et al. Transient Activation of Hedgehog Signaling Inhibits Cellular Senescence and Inflammation in Radiated Swine Salivary Glands through Preserving Resident Macrophages. Int J Mol Sci 22:N/A (2021). PubMed: 34948290
- Konitsiotis AD et al. Attenuation of hedgehog acyltransferase-catalyzed sonic Hedgehog palmitoylation causes reduced signaling, proliferation and invasiveness of human carcinoma cells. PLoS One 9:e89899 (2014). WB ; Human . PubMed: 24608521
- Kagawa H et al. A novel signaling pathway mediated by the nuclear targeting of C-terminal fragments of Mammalian patched 1. PLoS One 6:e18638 (2011). ICC/IF ; Mouse . PubMed: 21533246
- Chang SC et al. Two distinct sites in sonic hedgehog combine for heparan sulfate interactions and cell signaling functions. J Biol Chem 286:44391-402 (2011). WB ; Human . PubMed: 22049079
