重组Anti-Parathyroid Hormone抗体[SP151] (ab130759)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP151] to Parathyroid Hormone
- Suitable for: IHC-P, mIHC
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Parathyroid Hormone抗体[SP151]
参阅全部 Parathyroid Hormone 一抗 -
描述
兔单克隆抗体[SP151] to Parathyroid Hormone -
宿主
Rabbit -
经测试应用
适用于: IHC-P, mIHCmore details -
种属反应性
与反应: Human
预测可用于: Cow, Dog, Pig, Monkey -
免疫原
Synthetic peptide within Human Parathyroid Hormone aa 50 to the C-terminus (internal sequence). The exact sequence is proprietary.
Database link: P01270 -
阳性对照
- IHC-P: Human parathyroid gland tissue. mIHC: Human parathyroid gland tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP151 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab130759于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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mIHC |
Use at an assay dependent concentration.
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说明 |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
mIHC
Use at an assay dependent concentration. |
靶标
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功能
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. -
疾病相关
Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. -
序列相似性
Belongs to the parathyroid hormone family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 280903 Cow
- Entrez Gene: 403986 Dog
- Entrez Gene: 5741 Human
- Entrez Gene: 399502 Pig
- Omim: 168450 Human
- SwissProt: P01268 Cow
- SwissProt: P52212 Dog
- SwissProt: P01270 Human
see all -
别名
- hPTH antibody
- Parathormone antibody
- Parathyrin antibody
see all
图片
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Fluorescence multiplex immunohistochemical analysis of the human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-Parathyroid Hormone (ab236229, magenta; Opal™690), anti-Cytochrome C (ab247438, green; Opal™520) and anti-FABP4 (ab92501, red; Opal™570) on human parathyroid gland. Panel B: anti-Cytochrome C stained on parathyroid oxyphil cells. Panel C: anti-Parathyroid Hormone stained on parathyroid chief cells. Panel D: anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of ab236229 at 1/200 dilution (5.065 μg/ml) for 10 mins, then ab247438 at 1/5000 dilution (0.195 μg/ml) and ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
This image was generated using ab236229, the same clone, but with a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (ab130759)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human parathyroid gland tissue sections labeling Parathyroid Hormone with ab130759 at 1/100 dilution (5.77 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human parathyroid gland, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab130759 for 10 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (ab130759)
ab130759, at a dilution of 1/100, staining Parathyroid Hormone in paraffin embedded human parathyroid tissue by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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