重组Anti-PAR6抗体[EPR12378] - N-terminal (ab180159)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12378] to PAR6 - N-terminal
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PAR6抗体[EPR12378] - N-terminal
参阅全部 PAR6 一抗 -
描述
兔单克隆抗体[EPR12378] to PAR6 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human PAR6 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NPB6 -
阳性对照
- HeLa, 293T, Jurkat, Y79 and fetal liver lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12378 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180159于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
1/10 - 1/100.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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说明 |
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IP
1/10 - 1/100. |
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
靶标
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功能
Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. -
组织特异性
Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta. -
序列相似性
Belongs to the PAR6 family.
Contains 1 OPR domain.
Contains 1 PDZ (DHR) domain.
Contains 1 pseudo-CRIB domain. -
结构域
The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
The OPR domain mediates interactions with MAP2K5.
The PDZ domain mediates the interaction with CRB3. -
细胞定位
Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions. - Information by UniProt
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数据库链接
- Entrez Gene: 50855 Human
- Entrez Gene: 56513 Mouse
- Entrez Gene: 307799 Rat
- Omim: 607484 Human
- SwissProt: Q9NPB6 Human
- SwissProt: Q9Z101 Mouse
- SwissProt: Q6B4M5 Rat
- Unigene: 112933 Human
see all -
别名
- 0710008C04Rik antibody
- 2610010A15Rik antibody
- Par 6 partitioning defective 6 C elegans homolog alpha antibody
see all
图片
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All lanes : Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal liver lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa -
Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing PAR6, using ab180159 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab180159 被引用在 3 文献中.
- Zhou Y et al. Congenital biliary atresia is correlated with disrupted cell junctions and polarity caused by Cdc42 insufficiency in the liver. Theranostics 11:7262-7275 (2021). PubMed: 34158849
- Johnson MB et al. Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. Nature 556:370-375 (2018). PubMed: 29643508
- Zuckerwise L et al. H19 long noncoding RNA alters trophoblast cell migration and invasion by regulating TßR3 in placentae with fetal growth restriction. Oncotarget 7:38398-38407 (2016). PubMed: 27223264