Anti-PAR4抗体(ab5787)
Key features and details
- Rabbit polyclonal to PAR4
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PAR4抗体
参阅全部 PAR4 一抗 -
描述
兔多克隆抗体to PAR4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to Human PAR4 aa 324-342.
Sequence:EQLKQENKTLLKVVGQLTR
(Peptide available asab5853) -
阳性对照
- WB: Rat spleen extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).
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说明 |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa). |
靶标
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功能
Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1. -
组织特异性
Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers. -
结构域
The leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.
The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells. -
翻译后修饰
Preferentially phosphorylated at the Thr-163 by PKC in cancer cells. -
细胞定位
Cytoplasm. Nucleus. Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 64513 Rat
- SwissProt: Q62627 Rat
- Unigene: 9127 Rat
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别名
- 2310001G03Rik antibody
- PAR 4 antibody
- PAR-4 antibody
see all
图片
实验方案
数据表及文件
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Datasheet download
文献 (5)
ab5787 被引用在 5 文献中.
- Guo H et al. PAR-4 overcomes chemo-resistance in breast cancer cells by antagonizing cIAP1. Sci Rep 9:8755 (2019). PubMed: 31217499
- Acharya M et al. A complex regulatory network of transcription factors critical for ocular development and disease. Hum Mol Genet 20:1610-24 (2011). IP . PubMed: 21282189
- Fernandez-Marcos PJ et al. Simultaneous inactivation of Par-4 and PTEN in vivo leads to synergistic NF-kappaB activation and invasive prostate carcinoma. Proc Natl Acad Sci U S A 106:12962-7 (2009). IHC-P ; Human . PubMed: 19470463
- Acharya M et al. Human PRKC apoptosis WT1 regulator is a novel PITX2-interacting protein that regulates PITX2 transcriptional activity in ocular cells. J Biol Chem 284:34829-38 (2009). WB, IHC-P ; Human, Mouse . PubMed: 19801652
- Chung H et al. IGF-I inhibition of apoptosis is associated with decreased expression of prostate apoptosis response-4. J Endocrinol 194:77-85 (2007). PubMed: 17592023