Anti-PAR-3/PARD3抗体[EP310Y] (ab40769)
![Western blot - Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769)](https://www.abcam.cn/ps/products/40/ab40769/Images/ab40769-53896-anti-par-3-pard3-antibody-ep310y-western-blot.jpg "Western blot - Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769)")
Key features and details
- Rabbit monoclonal [EP310Y] to PAR-3/PARD3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-PAR-3/PARD3抗体[EP310Y]
参阅全部 PAR-3/PARD3 一抗 -
描述
兔单克隆抗体[EP310Y] to PAR-3/PARD3 -
宿主
Rabbit -
特异性
This antibody is predicted to detect isoforms 2-7 based on sequence analysis. -
经测试应用
适用于: WBmore details
不适用于: ICC/IF,IHC or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: LNCap, HeLa, A431, MCF-7
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP310Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40769于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
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| 说明 |
|---|
|
WB
1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
靶标
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功能
Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the PAR3 family.
Contains 3 PDZ (DHR) domains. -
结构域
Contains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids. -
翻译后修饰
Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity. -
细胞定位
Endomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
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数据库链接
- Entrez Gene: 56288 Human
- Omim: 606745 Human
- SwissProt: Q8TEW0 Human
- Unigene: 131489 Human
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别名
- agouti signaling protein antibody
- ASIP antibody
- Atypical PKC isotype specific interacting protein antibody
see all
图片
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Western blot - Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769)All lanes : Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769) at 1/500 dilution
Lane 1 : LNCap
Lane 2 : HeLa
Lane 3 : A431
Lane 4 : MCF-7
Lysates/proteins at 10 µg per lane.
Predicted band size: 150 kDa
Observed band size: 150 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab40769 被引用在 2 文献中.
- Wu Z et al. lncRNA NEAT1 Downregulation Ameliorates the Myocardial Infarction of Mice by Regulating the miR-582-5p/F2RL2 Axis. Cardiovasc Ther 2022:4481360 (2022). PubMed: 36540097
- Cho JH et al. Identification of the novel substrates for caspase-6 in apoptosis using proteomic approaches. BMB Rep 46:588-93 (2013). PubMed: 24195789
