重组Anti-PAP抗体[SP313] (ab224802)
![Flow Cytometry - Anti-PAP antibody [SP313] (ab224802)](https://www.abcam.cn/ps/products/224/ab224802/Images/ab224802-286129-anti-prostatic-acid-phosphatase-antibody-sp313-flow-cytometry.jpg "Flow Cytometry - Anti-PAP antibody [SP313] (ab224802)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP313] to PAP
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PAP抗体[SP313]
参阅全部 PAP 一抗 -
描述
兔单克隆抗体[SP313] to PAP -
宿主
Rabbit -
经测试应用
适用于: IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: LNCaP cells. IHC-P: Human prostate adenocarcinoma tissue.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP313 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224802于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| Flow Cyt |
1/100.
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| 说明 |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
Flow Cyt
1/100. |
靶标
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序列相似性
Belongs to the histidine acid phosphatase family. -
细胞定位
Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles. - Information by UniProt
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数据库链接
- Entrez Gene: 55 Human
- Omim: 171790 Human
- SwissProt: P15309 Human
- Unigene: 433060 Human
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别名
- 5' NT antibody
- 5' nucleotidase antibody
- 5'-NT antibody
see all
图片
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Flow Cytometry - Anti-PAP antibody [SP313] (ab224802)
Flow cytometric analysis of LNCaP (human prostate cancer cell line) cells labeling PAP with ab224802 at 1/100 dilution (green) compared with a rabbit IgG negative control (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [SP313] (ab224802)](https://www.abcam.cn/ps/products/224/ab224802/Images/ab224802-286128-anti-prostatic-acid-phosphatase-antibody-sp313-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [SP313] (ab224802)Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PAP using ab224802 at 1/100 dilution in immunohistochemical analysis.
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![Anti-PAP antibody [SP313] (ab224802)](https://www.abcam.cn/ps/products/224/ab224802/Images/ab224802-3-benefits-of-recombinant-antibodies.png)
Anti-PAP antibody [SP313] (ab224802)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224802 尚未被引用在任何文献中。