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重组RabMAb

重组Anti-Pancreatic Polypeptide抗体[EPR23320-10] - BSA and Azide free (ab273055)

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Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)
  • Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)
  • Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23320-10] to Pancreatic Polypeptide - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

概述

  • 产品名称

    Anti-Pancreatic Polypeptide抗体[EPR23320-10] - BSA and Azide free
    参阅全部 Pancreatic Polypeptide 一抗

  • 描述

    兔单克隆抗体[EPR23320-10] to Pancreatic Polypeptide - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, IHC-Frmore details
    不适用于: ICC/IF,IP or WB

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human, mouse and rat pancreas tissue. IHC-Fr: Mouse and rat pancreas tissue.

  • 常规说明

    ab273055 is the carrier-free version of ab272732.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • 存储溶液

    pH: 7.2
    Constituent: PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR23320-10

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273055于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
应用说明
Is unsuitable for ICC/IF,IP or WB.

靶标

  • 相关性

    Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.

  • 细胞定位

    Secreted

  • 数据库链接

    • 别名

      • Pancreatic polypeptide antibody
      • Pancreatic polypeptide Y antibody
      • Pancreatic prohormone antibody
      • PNP antibody
      • PP antibody
      • PPY antibody
      • prepro-PP (prepropancreatic polypeptide) antibody
      see all

    图片

    • Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)
      Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat pancreas tissue labeling Pancreatic Polypeptide with ab272732 at 1/1000 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution (Green). Positive staining on PP cells in rat pancreatic islet is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272732).

    • Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)
      Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling Pancreatic Polypeptide with ab272732 at 1/1000 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution (Green). Positive staining on PP cells in mouse pancreatic islet is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272732).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)

      Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Pancreatic Polypeptide with ab272732 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on PP cells of rat pancreas. The section was incubated with ab272732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272732).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)

      Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Pancreatic Polypeptide with ab272732 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on PP cells of mouse pancreas (PMID: 27270601). The section was incubated with ab272732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272732).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Pancreatic Polypeptide with ab272732 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on PP cells of human pancreas. The section was incubated with ab272732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272732).

    • Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)
      Anti-Pancreatic Polypeptide antibody [EPR23320-10] - BSA and Azide free (ab273055)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab273055？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273055 尚未被引用在任何文献中。

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