Anti-Pancreatic Polypeptide抗体(ab113694)
Key features and details
- Rabbit polyclonal to Pancreatic Polypeptide
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Pancreatic Polypeptide抗体
参阅全部 Pancreatic Polypeptide 一抗 -
描述
兔多克隆抗体to Pancreatic Polypeptide -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Horse, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human pancreas tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113694于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 100064909 Horse
- Entrez Gene: 5539 Human
- Entrez Gene: 713988 Macaque monkey
- Omim: 167780 Human
- SwissProt: P68010 Horse
- SwissProt: P01298 Human
- SwissProt: P33684 Macaque monkey
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别名
- Pancreatic polypeptide antibody
- Pancreatic polypeptide Y antibody
- Pancreatic prohormone antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody (ab113694)IHC image of Pancreatic Polypeptide staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113694, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab113694 被引用在 8 文献中.
- Boehmer BH et al. Chronic Fetal Leucine Infusion Does Not Potentiate Glucose-Stimulated Insulin Secretion or Affect Pancreatic Islet Development in Late-Gestation Growth-Restricted Fetal Sheep. J Nutr 151:312-319 (2021). PubMed: 33326574
- Yoshihara E et al. Immune-evasive human islet-like organoids ameliorate diabetes. Nature 586:606-611 (2020). PubMed: 32814902
- Boehmer BH et al. A Chronic Fetal Leucine Infusion Potentiates Fetal Insulin Secretion and Increases Pancreatic Islet Size, Vascularity, and ß Cells in Late-Gestation Sheep. J Nutr 150:2061-2069 (2020). PubMed: 32470982
- Menegaz D et al. Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell. Nat Metab 1:1110-1126 (2019). PubMed: 32432213
- Katoh MC et al. MafB Is Critical for Glucagon Production and Secretion in Mouse Pancreatic a Cells In Vivo. Mol Cell Biol 38:N/A (2018). PubMed: 29378833
- Moin ASM et al. Characterization of Non-hormone Expressing Endocrine Cells in Fetal and Infant Human Pancreas. Front Endocrinol (Lausanne) 9:791 (2018). PubMed: 30687234
- Phelps EA et al. Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation. Sci Rep 7:45961 (2017). PubMed: 28401888
- Xiao X et al. Transient Suppression of TGFß Receptor Signaling Facilitates Human Islet Transplantation. Endocrinology 157:1348-56 (2016). PubMed: 26872091