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Anti-Pancreatic Polypeptide抗体[18B11] (ab37273)

Submit a review Q&A (7)

Key features and details

  • Mouse monoclonal [18B11] to Pancreatic Polypeptide
  • Suitable for: RIA, ELISA
  • Reacts with: Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-Pancreatic Polypeptide抗体[18B11]
    参阅全部 Pancreatic Polypeptide 一抗

  • 描述

    小鼠单克隆抗体[18B11] to Pancreatic Polypeptide

  • 宿主

    Mouse

  • 经测试应用

    适用于: RIA, ELISAmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Pancreatic polypeptide analogue

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.40
    Constituents: 2.9% Sodium chloride, 0.0268% PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    18B11

  • 骨髓瘤

    x63-Ag8.653

  • 同种型

    IgG2a

  • 轻链类型

    kappa

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab37273于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
RIA
ELISA
说明
应用说明
ELISA: Use at an assay dependent dilution.
ab37285 can be used in combination with ab37273 in a sandwich ELISA with detection limits at approximately 10 pg/ml.
RIA: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

靶标

  • 相关性

    Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.

  • 细胞定位

    Secreted

  • 数据库链接

    • 别名

      • Pancreatic polypeptide antibody
      • Pancreatic polypeptide Y antibody
      • Pancreatic prohormone antibody
      • PNP antibody
      • PP antibody
      • PPY antibody
      • prepro-PP (prepropancreatic polypeptide) antibody
      see all

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab37273？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab37273 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Question

    Thank you for your answer. Yes, I’m interested in one monoclonal antibody against Pancreatic Polypetide, if you have more information with others in-house antibodies is better to me. I need to know the amino acid sequence target in that antibody to purchase, also I need it for coupling to beads then it should not contain in the solution matrix any additives as carbohydrates or proteins.

    Read More
    Answer

    Thank you for waiting for our reply.

    Finally, I have got some information regarding the immunogen sequence for ab112575 Anti-Pancreatic Polypeptide antibody [6].


    ab112475was indeed a duplicate and is now unpublished.

    Please be aware that the antibody has only been tested in ELISA so for and we can only guarantee this application. However, the buffer is carbohydrateand protein free so it may be suitablefor your experiments as well.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Could you confirm that sandwich ELISA is an untested application for both ab37285 and ab37273? Which means that purchasing two of these antibodies and submitting a test report on the sandwich ELISA would later, entitle the customers to two free antibodies?

    Read More
    Answer

    Yes, if your customer purchases both and submits a review for each, then they can receive two free antibodies. We will need to issue you two codes, or the customer directly, which they then need to include in the reviews, one code for each review of each antibody.

    One potential difficulty, which has already been pointed out, is that the detection antibody will either need to be directly conjugated to an enzyme, fluorochrome or biotin via the EasyLink kits we offer or it will need to be followed by a conjugated IgG sub-class-specific secondary antibody, since both antibodies are mouse IgG. Antibody ab37285 is IgG1, and ab37286 is IgG2a.

    Please let me know if you or your customer have any questions, and if they are still intersted, and I will send you or the customer the two codes. I believe we have already sent information regarding the EasyLink conjugation kits but I will be happy to re-send it.



    Read More

    Question

    I suppose our customer is experiencing difficulty understanding the pdf file/attachment above in constructing an Eliza sandwich from using the two antibodies, ab37285 and ab37273.

    The customer would like to know how to construct an Eliza sandwich using the two antibody pairing above, would you be able to recommend the method, should there be any other way different to the attached file above. (or do you think the file attachment above is not an appropriate instruction to follow)?

    Read More
    Answer

    Thank you for your email.

    I also have heard back from the supplying lab who let me know thatab37285 was used as capture antibody at 1 µg/ml, and thatbiotinylated ab37273 (which is however notoffered by supplying lab) was used as detection antibody at 0.1 µg/ml. These are only guidelines for the dilutions. I do not have any further protocol information.

    We have several conjugation kits that the customer could choose from (https://www.abcam.com/EasyLink). The biotinylation would not be exclusive, and other conjugations can be used as well - depending on the detection system the customer has available to him.
    For biotin we have for instance EasyLink Biotin (type A) Conjugation Kit (3 x 100µg Biotin) (ab102864): https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100µg-Biotin-ab102864.html

    We do not list sandwich ELISA as tested application as the antibodies per se (unconjugated) were not tested in this way. I can offer your participation in our testing discount program if the customer would like to try the antibodies in sandwich ELISA.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
    The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
    This programapplies to ELISA kits and other kits we offer.

    The protocol should be more clear now that the customer knows which antibody is for capturing and for detection. The secondary antibody will need to be left out as the detection antibody should conjugated.

    I hope this answers all the questions. Please let me know if I can be offurther help.

    Read More

    Question

    Dear tech support

    I have an enquiry from a customer as below and promptly seek your assistance:

    It is good to know ab37285 and ab37273 have been tested together in a sandwich ELISA. I notice, however, they are both mouse-raised antibodies; anti-IgG will not be able to distinguish between capture; detection antibodies. Do you stock a biotinylated version of either of these antibodies? How were the antibodies conjugated to produce signal in the test assays you as per the datasheet provided above?

    Please suggest how I can use the two antibodies to form a matching Elisa sandwich pair so as to produce signal in the test assay?

    Looking forward to your response!

    Thank you heaps heaps

    Kind regards,

    Read More
    Answer

    Thank you for your enquiry.

    I can confirm that ab37285 and ab37273 have been tested together in a sandwich ELISA. Unfortunately, we have no biotinylated version of these clones for sale. However, we do havesome biotin conjugation kits that may be helpful. This could be used to conjugate the detection antibody. I can recommend to review the online datasheets for further information.

    ab102864 EasyLink Biotin (type A) Conjugation Kit (3 x 100µg Biotin)
    https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100g-Biotin-ab102864.html (or use the following: https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100g-Biotin-ab102864.html).

    ab102865 EasyLink Biotin (type A) Conjugation Kit (3 x 10µg Biotin)
    https://www.abcam.com/Biotin-type-A-Conjugation-Kit-ab102865.html (or use the following: https://www.abcam.com/Biotin-type-A-Conjugation-Kit-ab102865.html).

    ab102866 EasyLink Biotin (type B) Conjugation Kit (1 x 1mg Biotin)
    https://www.abcam.com/EasyLink-Biotin-type-B-Conjugation-Kit-1-x-1mg-Biotin-ab102866.html (or use the following: https://www.abcam.com/EasyLink-Biotin-type-B-Conjugation-Kit-1-x-1mg-Biotin-ab102866.html).

    ab102867 EasyLink Biotin (type B) Conjugation Kit (3 x 100µg Biotin)
    https://www.abcam.com/Biotin-type-B-Conjugation-Kit-ab102867.html (or use the following: https://www.abcam.com/Biotin-type-B-Conjugation-Kit-ab102867.html).

    ab102868 EasyLink Biotin (type B) Conjugation Kit (3 x 10µg Biotin)
    https://www.abcam.com/EasyLink-Biotin-type-B-Conjugation-Kit-3-x-10g-Biotin-ab102868.html (or use the following: https://www.abcam.com/EasyLink-Biotin-type-B-Conjugation-Kit-3-x-10g-Biotin-ab102868.html).

    ab102863 EasyLink Biotin (type A) Conjugation Kit (1 x 1mg Biotin)
    https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-1-x-1mg-Biotin-ab102863.html (or use the following: https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-1-x-1mg-Biotin-ab102863.html).

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Question

    Thank you for your answer. Yes, I’m interested in one monoclonal antibody against Pancreatic Polypetide, if you have more information with others in-house antibodies is better to me. I need to know the amino acid sequence target in that antibody to purchase, also I need it for coupling to beads then it should not contain in the solution matrix any additives as carbohydrates or proteins.

    Read More
    Answer

    Thank you for your patience.

    Unfortunately, it looks like we have a duplication in our catalog and I have asked our datasheet team to look into this. Therefore, we may just have one more mouse monoclonal. In the meanwhile I have asked the lab for more info about the epitopes of those products, too.

    I will come back to you as soon asI know more and I am sorry for the delay of your case.

    Read More

    Question

    I need your support to choose the better antibody for my experiment. Only to know if the PP molecule used to produce the antibody was the PP N-termnal, PP C-terminal or the whole PP peptide would be great to take my decision.

    Read More
    Answer

    Thank you for your reply.


    I am sorry that I cannot help you with those two antibodies, but maybe I can assist you with our other remaining five antibodies for this target? We have two more mouse monoclonal and three polyclonal antibodies. I could see if we have more information regarding the immunogen with those five and if they would fit your experiment. Are you looking for an antibody which will bind in a specific area or do you want to detect the whole protein?



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I’m interested in acquire Anti-pancreatic polypeptide antibody, however I found in your website you supply two different antibodies defined as HYB 347-06 and HYB 347-07 with different epitope specificity. Do you know what amino acid sequences are involved in each antibody target?.

    Read More
    Answer

    Thank you for your inquiry.

    Unfortunately, we are not able to release the immunogen sequences for this particular antibodies (ab37273 Anti-Pancreatic Polypeptide antibody [HYB 347-07]and ab37285 Anti-Pancreatic Polypeptide antibody [HYB 347-06])as it is proprietary information.

    We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.

    Should you have any further questions, please do not hesitate to contact us.

    Read More

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