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重组RabMAb

重组Anti-Pancreatic Lipase/PTL抗体[EPR6276] (ab124915)

Submit a review Q&A (2)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
  • Western blot - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
  • OI-RD Scanning - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
  • Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6276] to Pancreatic Lipase/PTL
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Pancreatic Lipase/PTL抗体[EPR6276]
    参阅全部 Pancreatic Lipase/PTL 一抗

  • 描述

    兔单克隆抗体[EPR6276] to Pancreatic Lipase/PTL

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: IP

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human Pancreatic Lipase/PTL aa 350-450. The exact sequence is proprietary.

  • 阳性对照

    • Human pancreas and Human small intestine lysates; Human pancreas tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124915于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 51 kDa).
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 51 kDa).
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP.

靶标

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)

    ab124915, at 1/500 dilution, staining Pancreatic Lipase/PTL in paraffin embedded Human pancreas tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
    Western blot - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
    All lanes : Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915) at 1/10000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : Human small intestine lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 51 kDa

  • OI-RD Scanning - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
    OI-RD Scanning - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
    Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)

文献 (2)

发表研究结果有使用 ab124915？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab124915 被引用在 2 文献中.

  • Prangenberg J  et al. Fox sign in a case of terminal stage pancreatic cancer and suggestions for diagnosis. Forensic Sci Med Pathol 17:486-492 (2021). PubMed: 34191249
  • Courelli V  et al. Digestive Enzyme Activity and Protein Degradation in Plasma of Heart Failure Patients. Cell Mol Bioeng 14:583-596 (2021). PubMed: 34900012

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Dear Tech support

Could you speculate, based on immunogen sequence, the chances of good antibody specificity against Rat for WB and IHC-P?

Thank you very much for the support.
Regards,

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Answer

Thank you for your enquiry.

I am sorry to confirm that both Pancreatic Lipase antibodies you mention (ab124915, ab133556) are stated as 'does not react with mouse' and we would therefore not expect them to detect in this species,

Regrettably as far as we are aware, non of the other pancreatic lipase antibodies has ever been tested in IHC-P or rat. All tested applications and tested species covered by the 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

I can suggest to consider trying ab306746. The alignment of the immunogen with the rat sequence is high, and so we would predict this antibody would detect in rat. However, please note we are not able to guarantee this without further testing.

If the customer would like to test ab306746 antibody in both rat and IHC-P, please do not hesitate to contact us again prior to the purchase by replying to this message as they will be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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Question

I tried to look at the datasheet of antibody ab306746 and got this answer:

ab306746 doesn't match any products in our catalog. Try broadening your search or use alternative keywords.

Did you mean to recommend another antibody?

Thank you for the help.

Regards,

Read More
Answer

Thank you for your message.

My apologies. This should have been ab30746:

https://www.abcam.com/Pancreatic-LipasePTL-antibody-8F195-ab30746.html (or use the following: https://www.abcam.com/Pancreatic-LipasePTL-antibody-8F195-ab30746.html).

I hope this will be more helpful. Please do let me know if you have any further questions.

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