JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB6176

Anti-pan Synuclein抗体

Anti-pan Synuclein antibody

4

(4 Reviews)

|

(18 Publications)

Rabbit Polyclonal Alpha-synuclein antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Rat, Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human SNCA aa 1-50.

查看别名

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (AB6176)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (AB6176)

ICC/IF image of ab6176 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6176, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human, Rat

应用

IHC-P, ICC/IF, IHC-Fr, WB

applications

免疫原

Synthetic Peptide within Human SNCA aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P37840

特异性

This antibody recognises all 3 forms of the synucleins.

style
left-column

替代产品

Primary Antibodies

AB53726

Anti-pan Synuclein antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Synuclein antibody (AB53726)

  • 5
  • 2

搭配使用产品

Assay Kits

AB260052

Human Alpha-synuclein ELISA Kit

KO Validated|Recombinant|SimpleStep

ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

  • 5
  • 2
Primary Antibodies

AB51253

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

  • 5
  • 9
Primary Antibodies

AB278054

Anti-GFAP antibody [RM1003] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [RM1003] - Astrocyte Marker (AB278054)

  • 0
  • 0
Primary Antibodies

AB137869

Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

  • 5
  • 9
Primary Antibodies

AB221908

Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (AB221908)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯度
Whole antiserum
存储溶液
pH: 7 - 7.4
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Add glycerol to a final volume of 50% for extra stability and aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Pan Synuclein also known as alpha beta and gamma synucleins are small proteins with molecular masses around 14-19 kDa. These proteins are expressed largely in the central nervous system and are also present in other tissues such as the peripheral nervous system and certain types of muscles. They share structural similarities and comprise a family often involved with neurons where they play roles in various cellular processes.
Biological function summary

Synucleins participate in the regulation of synaptic function and plasticity influencing neurotransmitter release and membrane dynamics. They do not form a specific complex but can interact with lipid membranes and various cellular proteins affecting vesicular transport processes. These proteins hold influence over essential cellular functions that depend on synaptic activity and efficiency.

Pathways

Synucleins involve themselves in important neuronal pathways such as those controlling dopamine neurotransmission and regulation of mitochondrial functions. They interact with key proteins like parkin and ubiquitin which participate in protein degradation pathways. Synucleins' roles in these pathways highlight their impact on both synaptic transmission and energy metabolism within neurons.

Synucleins have strong connections to neurodegenerative disorders like Parkinson's disease and dementia with Lewy bodies. In these disorders alpha-synuclein in particular forms aggregates known as Lewy bodies contributing to neuronal dysfunction. These aggregates interact with proteins such as tau and amyloid-beta further linking synucleins to the pathology of these disorders. Understanding these interactions provides insights into potential targets for therapeutic interventions.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).
See full target information SNCA
style
left-column

文献 (18)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107419 PubMed38815862

2024

Neuronal activity promotes secretory autophagy for the extracellular release of α-synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshitsugu Nakamura,Taiki Sawai,Kensuke Kakiuchi,Shigeki Arawaka

Frontiers in cell and developmental biology 9:774650 PubMed34901020

2021

Synuclein Regulates Synaptic Vesicle Clustering and Docking at a Vertebrate Synapse.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlyn E Fouke,M Elizabeth Wegman,Sarah A Weber,Emily B Brady,Cristina Román-Vendrell,Jennifer R Morgan

Neurobiology of disease 151:105256 PubMed33429042

2021

Doxycycline inhibits α-synuclein-associated pathologies in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Dominguez-Meijide,Valeria Parrales,Eftychia Vasili,Florencia González-Lizárraga,Annekatrin König,Diana F Lázaro,Annie Lannuzel,Stéphane Haik,Elaine Del Bel,Rosana Chehín,Rita Raisman-Vozari,Patrick P Michel,Nicolas Bizat,Tiago Fleming Outeiro

Journal of neurochemistry 156:674-691 PubMed32730640

2020

Dopamine promotes the neurodegenerative potential of β-synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Anupam Raina,Kristian Leite,Sofia Guerin,Sameehan U Mahajani,Kalyan S Chakrabarti,Diana Voll,Stefan Becker,Christian Griesinger,Mathias Bähr,Sebastian Kügler

Cell death & disease 10:898 PubMed31776327

2019

Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Sameehan Mahajani,Anupam Raina,Claudia Fokken,Sebastian Kügler,Mathias Bähr

The Journal of biological chemistry 294:9973-9984 PubMed31092553

2019

C-terminal α-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan P McGlinchey,Shannon M Lacy,Katherine E Huffer,Nahid Tayebi,Ellen Sidransky,Jennifer C Lee

Neurobiology of disease 124:276-288 PubMed30381260

2018

Development of an aggregate-selective, human-derived α-synuclein antibody BIIB054 that ameliorates disease phenotypes in Parkinson's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Weihofen,YuTing Liu,Joseph W Arndt,Christian Huy,Chao Quan,Benjamin A Smith,Jean-Luc Baeriswyl,Nicole Cavegn,Luzia Senn,Lihe Su,Galina Marsh,Pavan K Auluck,Fabio Montrasio,Roger M Nitsch,Warren D Hirst,Jesse M Cedarbaum,R Blake Pepinsky,Jan Grimm,Paul H Weinreb

PloS one 12:e0184262 PubMed28877262

2017

The ER retention protein RER1 promotes alpha-synuclein degradation via the proteasome.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo-Jin Park,Daniel Ryu,Mayur Parmar,Benoit I Giasson,Nikolaus R McFarland

Human molecular genetics 25:275-90 PubMed26586132

2015

Yeast reveals similar molecular mechanisms underlying alpha- and beta-synuclein toxicity.

Applications

ICC/IF

Species

Human

Sandra Tenreiro,Rita Rosado-Ramos,Ellen Gerhardt,Filippo Favretto,Filipa Magalhães,Blagovesta Popova,Stefan Becker,Markus Zweckstetter,Gerhard H Braus,Tiago Fleming Outeiro

Journal of neuropathology and experimental neurology 72:624-45 PubMed23771222

2013

α-Synuclein vaccination prevents the accumulation of parkinson disease-like pathologic inclusions in striatum in association with regulatory T cell recruitment in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Vanesa Sanchez-Guajardo,Ambra Annibali,Poul Henning Jensen,Marina Romero-Ramos
View all publications
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com