Anti-pan PDK抗体[3H5BE12] (ab115321)
![Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)](https://www.abcam.cn/ps/products/115/ab115321/Images/ab115321-127377-anti-pan-pdk-antibody-3h5be12-western-blot.jpg "Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)")
Key features and details
- Mouse monoclonal [3H5BE12] to pan PDK
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
概述
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产品名称
Anti-pan PDK抗体[3H5BE12] -
描述
小鼠单克隆抗体[3H5BE12] to pan PDK -
宿主
Mouse -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein. This information is considered to be commercially sensitive.
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常规说明
PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯度
Proprietary Purification -
纯化说明
Near homogeneity as judged by SDS-PAGE (>95% purity). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Primary antibody说明
PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH. -
克隆
单克隆 -
克隆编号
3H5BE12 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115321于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.
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| 说明 |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. |
图片
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Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)All lanes : Anti-pan PDK antibody [3H5BE12] (ab115321) at 1 µg/ml
Lane 1 : human heart mitochondria at 20 µg
Lane 2 : human liver mitochondria at 10 µg
Lane 3 : rat liver mitochondria at 10 µg
Lane 4 : mouse liver mitochondria at 10 µg
Lane 5 : PDK1 recombinant protein ab110359
at 0.1 µg
Lane 6 : PDK2 recombinant protein ab110354 at 0.1 µg
Lane 7 : PDK3 recombinant protein ab110355 at 0.1 µg
Lane 8 : PDK4 recombinant protein ab110356 at 0.1 µg
Lane 9 : PDK1 recombinant protein ab86986 at 0.1 µg
Secondary
All lanes : GAM IR 800 1:5000
Performed under reducing conditions.
Predicted band size: 49 kDa
ab115321 identifies all four isoforms of PDKs (PDK1, PDK2, PDK3, and PDK4) with differential affinity. PDK3 has the highest affinity, then PDK2 and 4, it has the lowest affinity to PDK1. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan PDK antibody [3H5BE12] (ab115321)](https://www.abcam.cn/ps/products/115/ab115321/Images/ab115321-127376-anti-pan-pdk-antibody-3h5be12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan PDK antibody [3H5BE12] (ab115321)IHC image of ab115321 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab115321, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)](https://www.abcam.cn/ps/products/115/ab115321/Images/ab115321-229070-anti-pan-pdk-antibody-3h5be12-western-blot.jpg)
Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)This image is courtesy of an anonymous AbreviewWestern blot analysis of human ovarian cancer cell lines whole cell lysate (30µg/lane) labelling pan PDK with ab115321 at 1/1000. A HRP-conjugated goat anti-mouse IgG polyclonal (1/10000) was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab115321 被引用在 3 文献中.
- Zhang C et al. ISGylation of EMD promotes its interaction with PDHA to inhibit aerobic oxidation in lung adenocarcinoma. J Cell Mol Med 26:5078-5094 (2022). PubMed: 36071546
- Xiao ZD et al. Energy stress-induced lncRNA FILNC1 represses c-Myc-mediated energy metabolism and inhibits renal tumor development. Nat Commun 8:783 (2017). PubMed: 28978906
- Koukourakis MI et al. Metabolic cooperation between co-cultured lung cancer cells and lung fibroblasts. Lab Invest 97:1321-1331 (2017). PubMed: 28846077