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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins

Anti-pan Cytokeratin抗体[PCK-26] (ab6401)

  • Datasheet
  • SDS
Reviews (20)Q&A (8)References (43)

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Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
  • Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)

Key features and details

  • Mouse monoclonal [PCK-26] to pan Cytokeratin
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-pan Cytokeratin抗体[PCK-26]
    参阅全部 pan Cytokeratin 一抗
  • 描述

    小鼠单克隆抗体[PCK-26] to pan Cytokeratin
  • 宿主

    Mouse
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Rat, Rabbit, Goat, Chicken, Cow, a wide range of other species
  • 免疫原

    Full length native protein (purified) corresponding to Human pan Cytokeratin.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 纯化说明

    Purified from TCS.
  • 克隆

    单克隆
  • 克隆编号

    PCK-26
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    unknown
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6401于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (5)
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration.

靶标

  • 相关性

    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • 细胞定位

    Cytoplasmic
  • 数据库链接

    • Entrez Gene: 374454 Human
    • Entrez Gene: 51350 Human
    • SwissProt: Q01546 Human
    • SwissProt: Q7Z794 Human
    • 别名

      • pan ck antibody
      • pan-ck antibody
      • panck antibody

    图片

    • Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
      Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
      Lane 1 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 2.5 µg/ml
      Lane 2 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 5 µg/ml
      Lane 3 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 10 µg/ml

      All lanes : HeLa cell lysate

      Secondary
      All lanes : Peroxidase-conjugated goat anti-mouse IgG


      This image was generated using the ascites version of the product.

    • Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
      Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)This image is courtesy of an Abreview submitted by Pawel Mazur

      All lanes: pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 µg)

      Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique

      Performed under non-reducing conditions.

      Blocking Step: 10% Milk for 1 hour at room temperature

      This image was generated using the ascites version of the product.

      See Abreview

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (43)

    发表研究结果有使用 ab6401?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6401 被引用在 43 文献中.

    • Chen L  et al. IFN-γ+ cytotoxic CD4+ T lymphocytes are involved in the pathogenesis of colitis induced by IL-23 and the food colorant Red 40. Cell Mol Immunol 19:777-790 (2022). PubMed: 35468944
    • Heid I  et al. Functional noninvasive detection of glycolytic pancreatic ductal adenocarcinoma. Cancer Metab 10:24 (2022). PubMed: 36494842
    • Cordero H  et al. Intrathymic differentiation of natural antibody-producing plasma cells in human neonates. Nat Commun 12:5761 (2021). PubMed: 34599177
    • Schutt CR  et al. Genomic and neoantigen evolution from primary tumor to first metastases in head and neck squamous cell carcinoma. Oncotarget 12:534-548 (2021). PubMed: 33796222
    • Cantoni C  et al. Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cells. Oncoimmunology 10:1879530 (2021). PubMed: 33758675
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 28 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cytokeratin antibody [PCK-26]

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Thymus)
    Permeabilization
    No
    Specification
    Thymus
    Blocking step
    Blocking Agent as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 17 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Synovial Tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6 & TRIS pH 9
    Specification
    Synovial Tissue
    Blocking step
    10% Donkey serum + 3% BSA as blocking agent as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Dylan Windell

    Verified customer

    提交于 Apr 23 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mouse Primary Tumor (Mammary Gland))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate Buffer
    Permeabilization
    No
    Specification
    Mouse Primary Tumor (Mammary Gland)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 26°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Francesco Elia Marino

    Verified customer

    提交于 Sep 25 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Normal kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    Normal kidney
    Blocking step
    (agent) for 15 minute(s) · Concentration: 3% · Temperature: 27°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 24 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
    Sample
    Pig Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    Yes - 0.05% Tween 20
    Fixative
    10% Formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Jesse Wu

    Verified customer

    提交于 Oct 30 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    Yes - 0.05% Tween 20
    Fixative
    10% Formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 29 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
    Sample
    Rabbit Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    Yes - 0.05% Tween 20
    Fixative
    10% Formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 29 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mouse lung)
    Specification
    Mouse lung
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Permeabilization
    Yes - Trion X-100
    Blocking step
    2% horse serum plus mouse Fab IgG fragment as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 28 2013

    Question

    I need specification (data) sheets, with specific protein concentrations per lot#, for various products that I used by Abcam. This information must be include in my file to be archived at work. ab16667 - Lot# GR59808-2 ab6401 - Lot# GR1281-5 I found generic data sheets online but I need the documented protein concentration for the actual lot numbers I used. Somebody misplaced the original paperwork that came with the products.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 01 2012

    Answer

    Thank you for contacting us.
    Attached are the two Certificates of Compliance you have requested.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Nov 01 2012

    Question

    To Whom It May Concern:

    I would like to ask you for anti-pan Cytokeratin antibody product: What is the different between anti-pan cytokeratin antibody(PCK-26) code # ab6401 and anti-pan cytokeratin antibody (PCK-26) code # ab11214. ?. I also would like to know more information for your product, anti-pan Cytokeratin antibody (AE1+AE3) prediluted code # ab961. Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 25 2012

    Answer

    Thank you for contacting us.

    The antibodies ab6401 and ab11214 are from the same clone [PCK-26], so they are the same antibody with similar binding characteristics. The difference is that ab6401 is not purified and is not conjugated while ab11214 is purified and conjugated to FITC.

    The mouse monoclonal [AE1+AE3] to pan Cytokeratin, prediluted, ab961 is already diluted and ready to use for cell and tissue staining right out of the vial. You receive 6 ml of this pre-diluted antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Jun 25 2012

    1-10 of 28 Abreviews or Q&A

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