重组Anti-pan Cytokeratin抗体[AE1+AE3]，prediluted (ab961)

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I can confirm that the Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961) can be used for applications without further dilutions.

However, every experiment is different and may need optimisation by the user. Some customers seem to get excellent results by diluting this prediluted antibody further. We would recommend to optimise the experiment by trying the undiluted antibody and if possible dilutions found in the literature.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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L'anticorps prédilué ab82612 (Anti-Cytokeratin [MNF116]) a une concentration entre 1 et 3 µg/ml.
L'anticorps ab28028 (Anti-Vimentin [Vim 3B4]) est un supernageant, la concentration en anticorps ne peut donc pas être déterminée précisement mais elle est estimée entre 1 et 3 mg/ml.
Malheureusement le laboratoire qui produit l'anticorps ab961 (Anti-pan Cytokeratin antibody [AE1+AE3]) ne désire pas communiquer la concentration de ce produit. Nous sommes désolés de ne pas pouvoir vous fournir cette information, sachez cependant que nous avons au catalogue une référence équivalente, non-prédiluée, ab80826, à 1 mg/ml, https://www.abcam.com/ab80826.




J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Thank you for contacting us.

The antibodies ab6401 and ab11214 are from the same clone [PCK-26], so they are the same antibody with similar binding characteristics. The difference is that ab6401 is not purified and is not conjugated while ab11214 is purified and conjugated to FITC.

The mouse monoclonal [AE1+AE3] to pan Cytokeratin, prediluted, ab961 is already diluted and ready to use for cell and tissue staining right out of the vial. You receive 6 ml of this pre-diluted antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry and patience. I was able to obtain the following information from the originators of these antibodies: For ab11213, "this product should recognise cytokeratins 1, 4, 5, 6, 8,10, 13, 18 and 19. Based on literature, respiratory epithelia expressses cytokeratins 5, 6, 7, and 8. Thus I would believe this antibody would prove suitable but I would not have any references available specific for the use of this antibody for rat respiratory epithelia." For ab8794, "we have tested this antibody on skin, colon, pancreas and several other epithelial tissues. We have not tested in respiratory epithelia in rat, although this antibody has broad species reactivity, reacts with mouse, rat bovine, others not tested." I haven't been able to obtain any additional information regarding ab961. If I do, I will pass it on to you. This antibody is a combination of 2 clones and recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52. Please contact us again if you have any additional questions.

According to the datasheet, this antibody recognizes mouse protein. However, we do not have data on mouse kidney tissue particularly. Please have a look at the published references, you may find some more information.