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AB22744

Anti-pan Cadherin 抗体 [mAbcam22744]

Anti-pan Cadherin antibody [mAbcam22744]

4

(13 Reviews)

|

(23 Publications)

Mouse Monoclonal N Cadherin antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 23 publications.

查看别名

CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin

6 Images
Western blot - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)
  • WB

Project9292****

Western blot - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)

All lanes:

Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/mL

All lanes:

Heart (Rat) Tissue Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution

Predicted band size: 125 kDa

false

Exposure time: 30s

Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)
  • ICC/IF

AbReview22768****

Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)

ab22744 staining pan Cadherin in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol, permeabilised in 0.5% (w/v) saponin and then blocked using 10% serum for 2 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 2 hours at 23°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. Counterstained with DAPI (blue).

Image courtesy of an anonymous Abreview.

Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)

ICC/IF image of ab22744 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22744, 5μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).

Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)

ICC/IF image of ab22744 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22744, 5μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).

Western blot - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)
  • WB

AbReview21803****

Western blot - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)

Blocking performed with 5% milk for 1 hour.
Primary diluted with PBS + 0.5% Tween20 and incubated for 12 hours at 4°C
Performed under denaturing conditions.

All lanes:

Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1/500 dilution

All lanes:

Mouse cultured cortical neurons at 20 µg

Secondary

All lanes:

HRP-conjugated Goat Anti-Mouse IgG (H+L) polyclonal at 1/10000 dilution

Predicted band size: 125 kDa

true

Exposure time: 1min

This image is courtesy of an Anonymous abreview.

Western blot - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)
  • WB

Ap

Western blot - Anti-pan Cadherin antibody [mAbcam22744] (AB22744)

All lanes:

Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/mL

Lane 1:

Heart (Rat) Tissue Lysate (blocked with 5% Milk) at 20 µg

Lane 2:

Heart (Mouse) Tissue Lysate (blocked with 5% Milk) at 20 µg

Lane 3:

Heart (Human) Tissue Lysate - adult normal tissue (blocked with 5% Milk) at 20 µg

Lane 4:

Heart (Rat) Tissue Lysate (blocked with 3% Milk) at 20 µg

Lane 5:

Heart (Mouse) Tissue Lysate (blocked with 3% Milk) at 20 µg

Lane 6:

Heart (Human) Tissue Lysate - adult normal tissue (blocked with 3% Milk) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 125 kDa

Observed band size: 125 kDa,25 kDa,58 kDa

true

Exposure time: 8min

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

mAbcam22744

亚型

IgG1

轻链类型

kappa

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Detects a weaker band in human heart than in rat heart.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Abcam recommends using 3-5% milk as the blocking agent. Please see Western Blot data below.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Abcam recommends using 3-5% milk as the blocking agent. Please see Western Blot data below.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Abcam recommends using 3-5% milk as the blocking agent. Please see Western Blot data below.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式
Liquid
纯度
IgG fraction
存储溶液
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Pan Cadherin also known as cadherin pan antibodies or anti pan cadherin is a protein family that mediates cell-cell adhesion in tissues. This protein group includes several members like E-cadherin and N-cadherin which are integral in maintaining the structural integrity of tissues. Pan Cadherin proteins weigh around 120 kDa and are widely expressed in various cell types including epithelial and neural tissues. They possess repetitive amino acid sequences and are calcium-dependent playing an important role in the mechanical linking of cells.
Biological function summary

Pan Cadherin proteins mediate adhesion through forming homophilic interactions. These proteins are components of adherens junctions which are important structures in cellular adhesion. Within these complexes cadherins interact with catenins to link the cytoskeleton to cell membrane proteins maintaining cell polarity and tissue architecture. The function of cadherins extends to signaling roles that influence cell behavior and differentiation emphasizing their role in cellular communication.

Pathways

Pan Cadherin proteins participate in significant signaling mechanisms such as the Wnt signaling and epithelial-mesenchymal transition (EMT) pathways. In the Wnt signaling pathway cadherins influence cell fate decisions while in EMT they drive the remodeling of cellular structures important for tissue development. N-Cadherin is closely associated with Pan Cadherin in these pathways collaborating to mediate cellular transformations and migrations essential for embryogenesis and wound healing processes.

Pan Cadherin dysfunction correlates with cancer progression and metastasis. Aberrant cadherin expression contributes to the loss of cell adhesion promoting tumor invasion and metastasis. In particular disruptions in E-Cadherin frequently found in epithelial cancers highlight the critical role of Pan Cadherin in maintaining tissue homeostasis. Additionally N-Cadherin upregulation in various cancers illustrates its involvement in tumor aggressiveness further linking Pan Cadherin to pathogenesis.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone : upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
See full target information CDH2
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文献 (23)

Recent publications for all applications. Explore the full list and refine your search

Archiv der Pharmazie 356:e2200665 PubMed36949271

2023

Optimized synthesis and pharmacological evaluation of HCN channel inhibitor EC18.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Patberg,Tengiz Oniani,Paul Disse,Stefan Peischard,Laura Vinnenberg,Mehrnoush Zobeiri,Maria N Romanelli,Lisa Epping,Heinz Wiendl,Sven G Meuth,Petra Hundehege,Guiscard Seebohm,Thomas Budde,Anna Junker

Nature communications 13:5735 PubMed36175446

2022

Near-infrared phosphorescent carbon dots for sonodynamic precision tumor therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Bijiang Geng,Jinyan Hu,Yuan Li,Shini Feng,Dengyu Pan,Lingyan Feng,Longxiang Shen

Cellular and molecular life sciences : CMLS 79:440 PubMed35864219

2022

Virus-induced inhibition of cardiac pacemaker channel HCN4 triggers bradycardia in human-induced stem cell system.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Peischard,Melina Möller,Paul Disse,Huyen Tran Ho,Arie O Verkerk,Nathalie Strutz-Seebohm,Thomas Budde,Sven G Meuth,Patrick A Schweizer,Silke Morris,Lena Mücher,Verónica Eisner,Dierk Thomas,Karin Klingel,Karin Busch,Guiscard Seebohm

Frontiers in cardiovascular medicine 9:823133 PubMed35282351

2022

Common Variation in Regulatory Regions Highlights the Role of PPARγ as a Key Regulator of Endothelin .

Applications

Unspecified application

Species

Unspecified reactive species

Mauro Lago-Docampo,Carlos Solarat,Luis Méndez-Martínez,Adolfo Baloira,Diana Valverde

Nature biotechnology 40:74-85 PubMed34489600

2021

Integration of spatial and single-cell transcriptomic data elucidates mouse organogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

T Lohoff,S Ghazanfar,A Missarova,N Koulena,N Pierson,J A Griffiths,E S Bardot,C-H L Eng,R C V Tyser,R Argelaguet,C Guibentif,S Srinivas,J Briscoe,B D Simons,A-K Hadjantonakis,B Göttgens,W Reik,J Nichols,L Cai,J C Marioni

Nature communications 11:1556 PubMed32214092

2020

A common MET polymorphism harnesses HER2 signaling to drive aggressive squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ren Kong,Nur Afiqah Binte Mohamed Salleh,Richard Weijie Ong,Tuan Zea Tan,Nicholas L Syn,Robby Miguel Goh,Chee Wai Fhu,Daniel S W Tan,N Gopalakrishna Iyer,Srinivasaraghavan Kannan,Chandra S Verma,Yaw Chyn Lim,Ross Soo,Jingshan Ho,Yiqing Huang,Joline S J Lim,Benedict Junrong Yan,Min En Nga,Seng Gee Lim,H Phillip Koeffler,Soo Chin Lee,Dennis Kappei,Huynh The Hung,Boon Cher Goh

Science signaling 11: PubMed30154101

2018

Selective inhibition of Ca3.2 channels reverses hyperexcitability of peripheral nociceptors and alleviates postsurgical pain.

Applications

Unspecified application

Species

Unspecified reactive species

Sonja L Joksimovic,Srdjan M Joksimovic,Vesna Tesic,Agustin García-Caballero,Simon Feseha,Gerald W Zamponi,Vesna Jevtovic-Todorovic,Slobodan M Todorovic

Endocrinology 159:2683-2697 PubMed29771302

2018

Physical Linkage of Estrogen Receptor α and Aromatase in Rat: Oligocrine and Endocrine Actions of CNS-Produced Estrogens.

Applications

Unspecified application

Species

Unspecified reactive species

Emiliya M Storman,Nai-Jiang Liu,Martin W Wessendorf,Alan R Gintzler

International journal of endocrinology 2018:4537214 PubMed29686703

2018

Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Deng,Jianwen Zhang,Zhu Wang,Shengping Zhang,Fan Zhi,Hui Liang

Cellular physiology and biochemistry : internation 43:223-236 PubMed28854419

2017

Non-Genomic Action of Androgens is Mediated by Rapid Phosphorylation and Regulation of Androgen Receptor Trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Deng,Zeng Zhang,Yong Wu,Wang-Yang Yu,Jianwen Zhang,Zhi-Mao Jiang,Ying Zhang,Hui Liang,Yao-Ting Gui
View all publications
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我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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