AB119952

Anti-pan Actin 抗体 [4A4]

Name: pan Actin抗体[4A4] (ab119952)| Abcam中文官网
Brand: Abcam Limited
SKU: AB119952
Price: 4819 CNY
Availability: InStock

Anti-pan Actin antibody [4A4]

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(41 Publications)

Mouse Monoclonal alpha smooth muscle Actin antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P and reacts with Human, Rat, Mouse, African green monkey, Recombinant fragment - Human samples. Cited in 41 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA2 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

查看别名

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

6 Images

Flow Cytometry - Anti-pan Actin Antibody [4A4] (AB119952)

Flow Cyt

Unknown

Flow Cytometry - Anti-pan Actin Antibody [4A4] (AB119952)

Flow cytometric analysis of Hela cells using ab119952 at a dilution of 1/200 (green) and negative control (purple).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (AB119952)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (AB119952)

Immunohistochemical analysis of paraffin-embedded Human duodenum tissue using ab119952 at a dilution of 1/200 with DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (AB119952)

Immunohistochemical analysis of paraffin-embedded Human esophagus tissue using ab119952 at a dilution of 1/200 with DAB staining.

ELISA - Anti-pan Actin Antibody [4A4] (AB119952)

ELISA

Lab

ELISA - Anti-pan Actin Antibody [4A4] (AB119952)

1 ug per mL of recombinant protein ab157838 and ab157841 were immobilised in PBS on an ELISA plate overnight. After blocking in 5% BSA, primary antibody ab119952 was added in a concentration range of 0.017-1000 ng per mL.

Pre-absorbed secondary antibody goat anti-rabbit IgG H&L (ab97080) was used at 1/20000 dilution.

Unknown

Western blot - Anti-pan Actin Antibody [4A4] (AB119952)

All lanes:

Western blot - Anti-pan Actin Antibody [4A4] (ab119952) at 1/500 dilution

Lane 1:

HeLa cell lysate

Lane 2:

A431 cell lysate

Lane 3:

Jurkat cell lysate

Lane 4:

K562 cell lysate

Lane 5:

HEK293 cell lysate

Lane 6:

HepG2 cell lysate

Lane 7:

NIH3T3 cell lysate

Lane 8:

PC12 cell lysate

Lane 9:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

Predicted band size: 42 kDa

Observed band size: 42 kDa

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CiteAb

Western blot - Anti-pan Actin Antibody [4A4] (AB119952)

pan Actin western blot using anti-pan Actin Antibody [4A4] ab119952. Publication image and figure legend from Su, M., Fan, S., et al., 2020, Front Physiol, PubMed 32733269.

ab119952 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab119952 please see the product overview.

MiR-223 deficiency in diabetic platelet fails to suppress VSMC proliferation. Activated platelets (APs) from healthy subject (HS) and DM patients were cocultured with human VSMCs for 48 h. (A) Expression of differentiation markers ACTA2, CNN1, and TAGLN and dedifferentiation markers OPN, MYH10, KLF4, and KLF5 in control (Ctrl) VSMCs and APs cocultured with VSMCs (HS-APs, DM-APs) was detected with western blot analysis. (B,C) The rate of proliferation was determined by CCK8 assay and Brdu assay in VSMCs treated with APs pretreated by RNase A (degrades the RNA). Data are presented as mean ± SD (n = 6). Comparisons between more than two groups were analyzed using one-way ANOVA analysis with the Dunnett's multiple correction. *P < 0.05, **P < 0.01, ***P < 0.001 vs Ctrl (VSMCs without platelets); #P < 0.05, ##P < 0.01, ###P < 0.001 vs HS-APs. (D) The rate of proliferation was determined by EdU incorporation assay in VSMCs transfected with miR-223 mimic. Data are presented as mean ± SD (n = 6). Comparisons between two groups were analyzed using unpaired nonparametric Student's t-test.*P < 0.05 vs Ctrl (miR-NC).

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关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

4A4

亚型

IgG1

不含载体蛋白

反应种属

Mouse, Rat, Human, African green monkey

应用

WB, IHC-P, Flow Cyt, ELISA

applications

免疫原

Synthetic Peptide within Human ACTA2 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P62736

特异性

Actin proteins have very high homology. The immunogen for this product is based on the ACTA2 protein but this product will also detect Gamma actin, and likely other actin family members too.

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反应性数据

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

纯化说明

Purified from tissue culture supernatant.

存储溶液

Preservative: 0.05% Sodium azide Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The actin protein sometimes referred to as "pan actin" is an important component of the cytoskeleton and is fundamental in maintaining cell structure and integrity. Alternately called beta-actin or gamma-actin depending which variant is expressed this protein has a molecular weight of approximately 42 kDa. Actin is ubiquitously expressed in almost all eukaryotic cells where it forms dynamic networks that support the plasma membrane assist in cell movement and mediate intracellular transport.

Biological function summary

Actin polymerizes to form filaments (F-actin) from its monomeric forms (G-actin) within the cell. These filaments are critical for constructing the cytoskeleton and anchor cellular structures in place. Actin functions within the actin-myosin complex which facilitates muscle contraction and movement in non-muscle cells. Through this complex actin provides essential support in cellular processes such as cytokinesis and vesicle movement.

Pathways

Actin is important for pathways that control cell motility and structural rearrangements. It plays a significant role in the Rho-GTPase signaling pathway which regulates actin cytoskeleton dynamics. Actin interacts with other important proteins such as myosin and tropomyosin to facilitate these activities. Another important pathway involving actin is the Wnt signaling pathway where it aids in the regulation of cell proliferation and differentiation.

Actin is linked to conditions such as cancer metastasis and muscular dystrophies. In cancer actin's role in cell motility and invasion contributes to the metastatic spread of tumors. Actin dysregulation is also implicated in certain muscle disorders where mutations affect the formation and function of muscle fibers. In these contexts actin closely connects with dystrophin an important protein in muscle tissue integrity and any disruption can lead to disease manifestation.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA2

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文献 (41)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40857411

2025

Single-cell spatial transcriptomics unravels the cellular landscape of abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Guizhen Zhao,Chun-Seok Cho,Hongyu Liu,Yongha Hwang,Yichen Si,Myungjin Kim,Yongjie Deng,Yang Zhao,Chao Xue,Yanhong Guo,Lin Chang,Dogukan Mizrak,Bo Yang,Hyun Min Kang,Jifeng Zhang,Jun Hee Lee,Y Eugene Chen

Journal of pharmaceutical analysis 15:101120 PubMed39989903

2025

Hemodynamic disturbance and mTORC1 activation: Unveiling the biomechanical pathogenesis of thoracic aortic aneurysms in Marfan syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yuan Liu,Meili Wang,Junjun Liu,An-Qiang Sun,Chang-Shun He,Xin Cong,Wei Kong,Wei Li

Bioactive materials 45:181-200 PubMed39651397

2024

Engineering aortic valves via transdifferentiating fibroblasts into valvular endothelial cells without using viruses or iPS cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Tang,Fuxiang Wei,Weihua Qiao,Xing Chen,Chenyang Ji,Wanzhi Yang,Xinyu Zhang,Sihan Chen,Yanyan Wu,Mingxing Jiang,Chenyu Ma,Weiqiang Shen,Qi Dong,Hong Cao,Minghui Xie,Ziwen Cai,Li Xu,Jiawei Shi,Nianguo Dong,Junwei Chen,Ning Wang

Cardiovascular diabetology 23:381 PubMed39462409

2024

Endothelial KLF11 is a novel protector against diabetic atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Guizhen Zhao,Yang Zhao,Wenying Liang,Haocheng Lu,Hongyu Liu,Yongjie Deng,Tianqing Zhu,Yanhong Guo,Lin Chang,Minerva T Garcia-Barrio,Y Eugene Chen,Jifeng Zhang

Frontiers in cell and developmental biology 12:1279932 PubMed38434619

2024

Characterization of cardiac fibroblast-extracellular matrix crosstalk across developmental ages provides insight into age-related changes in cardiac repair.

Applications

Unspecified application

Species

Unspecified reactive species

Luke R Perreault,Mark C Daley,Matthew C Watson,Sagar Rastogi,Ajith Jaiganesh,Elizabeth C Porter,Breanna M Duffy,Lauren D Black

BMC cancer 23:1198 PubMed38057830

2023

Cancer-associated fibroblasts induce sorafenib resistance of hepatocellular carcinoma cells through CXCL12/FOLR1.

Applications

Unspecified application

Species

Unspecified reactive species

Jiali Zhao,En Lin,Zirui Bai,Yingbin Jia,Bo Wang,Yihua Dai,Wenfeng Zhuo,Guifang Zeng,Xialei Liu,Chaonong Cai,Peiping Li,Baojia Zou,Jian Li

Advanced healthcare materials 12:e2301683 PubMed37327023

2023

Panthenol Citrate Biomaterials Accelerate Wound Healing and Restore Tissue Integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Huifeng Wang,Chongwen Duan,Rebecca L Keate,Guillermo A Ameer

Cells 12: PubMed36980231

2023

Unleashed Actin Assembly in Capping Protein-Deficient B16-F1 Cells Enables Identification of Multiple Factors Contributing to Filopodium Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Jens Ingo Hein,Jonas Scholz,Sarah Körber,Thomas Kaufmann,Jan Faix

Biological & pharmaceutical bulletin 46:1-11 PubMed36596517

2023

Chemical Activation of Piezo1 Alters Biomechanical Behaviors toward Relaxation of Cultured Airway Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhi Luo,Kai Ni,Rong Gu,Youyuan Qin,Jia Guo,Bo Che,Yan Pan,Jingjing Li,Lei Liu,Linhong Deng

The Journal of clinical investigation 132: PubMed36066968

2022

BAF60c prevents abdominal aortic aneurysm formation through epigenetic control of vascular smooth muscle cell homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Guizhen Zhao,Yang Zhao,Haocheng Lu,Ziyi Chang,Hongyu Liu,Huilun Wang,Wenying Liang,Yuhao Liu,Tianqing Zhu,Oren Rom,Yanhong Guo,Lin Chang,Bo Yang,Minerva T Garcia-Barrio,Jiandie D Lin,Y Eugene Chen,Jifeng Zhang

View all publications

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Abcam Product Promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-pan Actin 抗体 [4A4]

Anti-pan Actin antibody [4A4]

Flow Cytometry - Anti-pan Actin Antibody [4A4] (AB119952)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (AB119952)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Actin Antibody [4A4] (AB119952)

ELISA - Anti-pan Actin Antibody [4A4] (AB119952)

Western blot - Anti-pan Actin Antibody [4A4] (AB119952)

Western blot - Anti-pan Actin Antibody [4A4] (AB119952)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性