Anti-PAK6 (phospho S165)抗体(ab41432)
Key features and details
- Rabbit polyclonal to PAK6 (phospho S165)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PAK6 (phospho S165)抗体
参阅全部 PAK6 一抗 -
描述
兔多克隆抗体to PAK6 (phospho S165) -
宿主
Rabbit -
特异性
Phospho-Ser165-specific. The antibody detects a 75 kDa band on SDS-PAGE immunoblots of human PAK6 recombinant protein phosphorylated with ERK2. This reactivity is specifically blocked by pre-incubation with phospho-Ser165-PAK6 peptide and also by treatment with lambda phosphatase. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human PAK6 (phospho S165).
Database link: Q9NQU5 -
常规说明
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was cross-adsorbed to unphosphorylated-Ser165-PAK6 peptide, then affinity purified using phospho-Ser165-PAK6 peptide (immunogen). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41432于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
|
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
靶标
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功能
The activated kinase acts on a variety of targets. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated when activated by CDC42/p21. - Information by UniProt
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数据库链接
- Entrez Gene: 56924 Human
- Entrez Gene: 214230 Mouse
- Entrez Gene: 296078 Rat
- Omim: 608110 Human
- SwissProt: Q9NQU5 Human
- SwissProt: Q3ULB5 Mouse
- Unigene: 513645 Human
- Unigene: 241857 Mouse
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别名
- CDKN1A activated kinase 6 antibody
- EC 2.7.1.37 antibody
- p21 activated protein kinase 6 antibody
see all
图片
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Western blot - Anti-PAK6 (phospho S165) antibody (ab41432)Lanes 1-4 : Anti-PAK6 antibody (ab41431) at 1/1000 dilution
Lanes 5-8 : Anti-PAK6 (phospho S165) antibody (ab41432) at 1/1000 dilution
Lanes 1 & 5 : Human PAK6 recombinant protein phosphorylated by ERK2
Lane 2 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing
Lane 3 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific peptide
Lane 4 : Human PAK6 recombinant protein phosphorylated by ERK2 with PAK6 N-terminal peptide (ab41431 immunogen)
Lane 6 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing
Lane 7 : Human PAK6 recombinant protein phosphorylated by ERK2 with phospho-S165-PAK6 peptide (ab41432 immunogen)
Lane 8 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific phospho-serine peptide
Predicted band size: 75 kDa
Observed band size: 75 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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