Anti-PAK5抗体(ab110069)
Key features and details
- Rabbit polyclonal to PAK5
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-PAK5抗体 -
描述
兔多克隆抗体to PAK5 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse
-
免疫原
Synthetic peptide from the C terminal region of Human PAK5.
-
阳性对照
- Human brain tissue; Rat brain cells extracts
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab110069 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110069于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 81 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 81 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
The activated kinase acts on a variety of targets. -
组织特异性
Predominantly expressed in brain. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated when activated by CDC42/p21. - Information by UniProt
-
数据库链接
- Entrez Gene: 57144 Human
- Entrez Gene: 241656 Mouse
- Omim: 608038 Human
- SwissProt: Q9P286 Human
- SwissProt: Q8C015 Mouse
- SwissProt: D4A280 Rat
- Unigene: 32539 Human
- Unigene: 131572 Mouse
-
别名
- EC 2.7.11.1 antibody
- KIAA1264 antibody
- MGC26232 antibody
see all
图片
-
Western blot - Anti-PAK5 antibody (ab110069)All lanes : Anti-PAK5 antibody (ab110069) at 1/500 dilution
Lane 1 : extracts from Rat brain cells
Lane 2 : extracts from Rat brain cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK5 antibody (ab110069)Immunohistochemical analysis of paraffin embedded Human brain tissue, using a 1/50 dilution of ab110069 with (right) or without (left) the immunizing peptide.
数据表及文件
-
SDS download
-
Datasheet download
文献 (10)
ab110069 被引用在 10 文献中.
- Gai SY & Yuan ZH Long non-coding RNA SOX21-AS1 promotes cell proliferation and invasion through upregulating PAK7 expression by sponging miR-144-3p in glioma cells. Neoplasma 67:333-343 (2020). PubMed: 31973536
- Rabieifar P et al. Normal mammary gland development after MMTV-Cre mediated conditional PAK4 gene depletion. Sci Rep 9:14436 (2019). PubMed: 31594963
- Wang W et al. miR-489 promotes apoptosis and inhibits invasiveness of glioma cells by targeting PAK5/RAF1 signaling pathways. Oncol Rep 42:2390-2401 (2019). PubMed: 31638257
- Han K et al. PAK5 overexpression is associated with lung metastasis in osteosarcoma. Oncol Lett 15:2202-2210 (2018). PubMed: 29434926
- Aburatani T et al. High expression of P21-activated kinase 5 protein is associated with poor survival in gastric cancer. Oncol Lett 14:404-410 (2017). PubMed: 28693183
- Pan YJ et al. MiR-106a-5p inhibits the cell migration and invasion of renal cell carcinoma through targeting PAK5. Cell Death Dis 8:e3155 (2017). WB ; Human . PubMed: 29072688
- Tabanifar B et al. PAK5 is auto-activated by a central domain that promotes kinase oligomerization. Biochem J 473:1777-89 (2016). PubMed: 27095851
- Gu X et al. Efficient inhibition of human glioma development by RNA interference-mediated silencing of PAK5. Int J Biol Sci 11:230-7 (2015). WB, IHC . PubMed: 25632266
- Han K et al. p21-activated kinase 7 is an oncogene in human osteosarcoma. Cell Biol Int 38:1394-402 (2014). IHC . PubMed: 25052921
- Fang ZP et al. P21-activated kinase 5 plays essential roles in the proliferation and tumorigenicity of human hepatocellular carcinoma. Acta Pharmacol Sin N/A:N/A (2013). PubMed: 23685956