重组Anti-PAK3抗体[EP797Y] (ab40808)
![Western blot - Anti-PAK3 antibody [EP797Y] (ab40808)](https://www.abcam.cn/ps/products/40/ab40808/Images/ab40808-54019-anti-pak3-antibody-ep797y-western-blot.jpg "Western blot - Anti-PAK3 antibody [EP797Y] (ab40808)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP797Y] to PAK3
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PAK3抗体[EP797Y]
参阅全部 PAK3 一抗 -
描述
兔单克隆抗体[EP797Y] to PAK3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human PAK3 aa 1-100 (N terminal). The exact sequence is proprietary.
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阳性对照
- Fetal brain lysate and human brain glioma.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP797Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40808于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 62 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 62 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt.
靶标
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相关性
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. PAK3 forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. It may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. A point mutation in this gene has been linked to nonsyndromic X-linked mental retardation. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 5063 Human
- Entrez Gene: 18481 Mouse
- Entrez Gene: 29433 Rat
- Omim: 300142 Human
- SwissProt: O75914 Human
- SwissProt: Q61036 Mouse
- SwissProt: Q62829 Rat
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别名
- Beta PAK antibody
- bPAK antibody
- CDKN1A antibody
see all
图片
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Western blot - Anti-PAK3 antibody [EP797Y] (ab40808)Anti-PAK3 antibody [EP797Y] (ab40808) at 1/50000 dilution + fetal brain lysate at 10 µg
Predicted band size: 62 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] (ab40808)](https://www.abcam.cn/ps/products/40/ab40808/Images/ab40808-54018-anti-pak3-antibody-ep797y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] (ab40808)ab40808 at a 1:100 dilution staining PAK3 in human brain glioma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-PAK3 antibody [EP797Y] (ab40808)](https://www.abcam.cn/ps/products/40/ab40808/Images/ab40808-3-benefits-of-recombinant-antibodies.png)
Anti-PAK3 antibody [EP797Y] (ab40808)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab40808 被引用在 3 文献中.
- Gao Z et al. PAK3 promotes the metastasis of hepatocellular carcinoma by regulating EMT process. J Cancer 13:153-161 (2022). PubMed: 34976179
- Lauterborn JC et al. Synaptic actin stabilization protein loss in Down syndrome and Alzheimer disease. Brain Pathol 30:319-331 (2020). PubMed: 31410926
- Zuo Z et al. LncRNA TTN-AS1/miR-134-5p/PAK3 axis regulates the radiosensitivity of human large intestine cancer cells through the P21 pathway and AKT/GSK-3β/β-catenin pathway. Cell Biol Int 44:2284-2292 (2020). PubMed: 32749739