Anti-PAK1 (phospho T423) + PAK2 (phospho T402 ) + PAK3 (phospho T436)抗体(ab62155)
Key features and details
- Rabbit polyclonal to PAK1 (phospho T423) + PAK2 (phospho T402) + PAK3 (phospho T436)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PAK1 (phospho T423) + PAK2 (phospho T402 ) + PAK3 (phospho T436)抗体 -
描述
兔多克隆抗体to PAK1 (phospho T423) + PAK2 (phospho T402) + PAK3 (phospho T436) -
宿主
Rabbit -
特异性
Detects endogenous levels of PAK1, PAK2 and PAK3 only when phosphorylated at threonine 423, threonine 402 and threonine 421 respectively (Human: Thr423/402/421; Mouse: Thr423/402/421; Rat: Thr422/402/421).
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经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide within Human PAK1 aa 350-450 (phospho T423). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13153 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62155于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/100.
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| 说明 |
|---|
|
IHC-P
1/50 - 1/100. |
靶标
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细胞定位
PAK1: Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation. PAK2: Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane. PAK3: Cytoplasmic -
数据库链接
- Entrez Gene: 5058 Human
- Entrez Gene: 5062 Human
- Entrez Gene: 5063 Human
- Entrez Gene: 29431 Rat
- Entrez Gene: 29432 Rat
- Entrez Gene: 29433 Rat
- GenBank: NP_002568.2 Human
- Omim: 300142 Human
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho T423) + PAK2 (phospho T402 ) + PAK3 (phospho T436) antibody (ab62155)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PAK1 (phospho T423) + PAK2 (phospho T402 ) + PAK3 (phospho T436) antibody (ab62155) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab62155 被引用在 1 文献中.
- Su N et al. Targeting P21-activated kinase suppresses proliferation and enhances chemosensitivity in T-cell lymphoblastic lymphoma. Blood Sci 5:249-257 (2023). PubMed: 37941919