Anti-PAK1 (phospho S204)抗体(ab79503)
Key features and details
- Rabbit polyclonal to PAK1 (phospho S204)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PAK1 (phospho S204)抗体
参阅全部 PAK1 一抗 -
描述
兔多克隆抗体to PAK1 (phospho S204) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human PAK1 (phospho S204).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79503于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 61 kDa.
|
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| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 61 kDa. |
|
IHC-P
1/50 - 1/100. |
靶标
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功能
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated when activated by CDC42/p21 and RAC1. -
细胞定位
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation. - Information by UniProt
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数据库链接
- Entrez Gene: 5058 Human
- Entrez Gene: 18479 Mouse
- Entrez Gene: 29431 Rat
- Omim: 602590 Human
- SwissProt: Q13153 Human
- SwissProt: O88643 Mouse
- SwissProt: P35465 Rat
- Unigene: 435714 Human
see all -
别名
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
see all
图片
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Western blot - Anti-PAK1 (phospho S204) antibody (ab79503)All lanes : Anti-PAK1 (phospho S204) antibody (ab79503) at 1/1000 dilution
Lane 1 : extracts from 3T3 cells (treated
with UV for 15 mins)
Lane 2 : extracts from 3T3 cells (treated
with UV for 15 mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho S204) antibody (ab79503)Immunohistochemistry analysis of paraffin-embedded human brain tissue, stained with ab79503, at 1/100 dilution, in the absence and presence of the immunising peptide
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab79503 被引用在 1 文献中.
- Vaquié A et al. Injured Axons Instruct Schwann Cells to Build Constricting Actin Spheres to Accelerate Axonal Disintegration. Cell Rep 27:3152-3166.e7 (2019). PubMed: 31189102