Anti-PAK1抗体(ab131522)
Key features and details
- Rabbit polyclonal to PAK1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PAK1抗体
参阅全部 PAK1 一抗 -
描述
兔多克隆抗体to PAK1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide conjugated to KLH, corresponding to a region around amino acids 210-214 (P-V-T-P-T) of Human PAK1 (NP_001122092.1).
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阳性对照
- Methanol-fixed HeLa cells; 293 and 3T3 cell extracts; Human breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131522于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 61 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/10 - 1/200.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 61 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/10 - 1/200. |
靶标
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功能
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated when activated by CDC42/p21 and RAC1. -
细胞定位
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation. - Information by UniProt
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数据库链接
- Entrez Gene: 5058 Human
- Entrez Gene: 18479 Mouse
- Omim: 602590 Human
- SwissProt: Q13153 Human
- SwissProt: O88643 Mouse
- Unigene: 435714 Human
- Unigene: 260227 Mouse
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别名
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
see all
图片
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All lanes : Anti-PAK1 antibody (ab131522) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 3T3 cell extract
Predicted band size: 61 kDa -
Immunofluorescent analysis of methanol-fixed HeLa cells labelling PAK1 with ab131522 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody (ab131522)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling PAK1 with ab131522 at 1/50 dilution. Right panel was preincubated with blocking peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab131522 被引用在 9 文献中.
- Tejeda-Munoz N et al. The PMA phorbol ester tumor promoter increases canonical Wnt signaling via macropinocytosis. Elife 12:N/A (2023). PubMed: 37902809
- Kumar S et al. Low dose radiation upregulates Ras/p38 and NADPH oxidase in mouse colon two months after exposure. Mol Biol Rep 50:2067-2076 (2023). PubMed: 36542238
- Brown TL et al. PAK1 Positively Regulates Oligodendrocyte Morphology and Myelination. J Neurosci 41:1864-1877 (2021). PubMed: 33478987
- Xu J et al. MiR-509-3p Induces Apoptosis and Affects the Chemosensitivity of Cervical Cancer Cells by Targeting the RAC1/PAK1/LIMK1/Cofilin Pathway. Chem Pharm Bull (Tokyo) 69:325-332 (2021). PubMed: 33790078
- Albrecht LV et al. GSK3 Inhibits Macropinocytosis and Lysosomal Activity through the Wnt Destruction Complex Machinery. Cell Rep 32:107973 (2020). PubMed: 32726636
- Wu XM et al. microRNA-542-5p protects against acute lung injury in mice with severe acute pancreatitis by suppressing the mitogen-activated protein kinase signaling pathway through the negative regulation of P21-activated kinase 1. J Cell Biochem 120:290-304 (2019). PubMed: 30216510
- Zhu M et al. Lentiviral-mediated ephrin B2 gene modification of rat bone marrow mesenchymal stem cells. J Int Med Res 47:3282-3298 (2019). PubMed: 31122164
- Grebenová D et al. PAK1, PAK1?15, and PAK2: similarities, differences and mutual interactions. Sci Rep 9:17171 (2019). PubMed: 31748572
- Wen X et al. ROCK1 and LIM Kinase Modulate Retrovirus Particle Release and Cell-Cell Transmission Events. J Virol 88:6906-21 (2014). PubMed: 24696479