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Cell Biology Apoptosis Intracellular Kinases

Anti-PAK1 + PAK2 + PAK3 (phospho S141)抗体(ab5247)

  • Datasheet
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Western blot - Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247)

    Key features and details

    • Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho S141)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-PAK1 + PAK2 + PAK3 (phospho S141)抗体
      参阅全部 PAK1 + PAK2 + PAK3 一抗
    • 描述

      兔多克隆抗体to PAK1 + PAK2 + PAK3 (phospho S141)
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Rat, Rabbit
    • 免疫原

      Synthetic peptide corresponding to Human PAK1 + PAK2 + PAK3 (phospho S141).

    • 阳性对照

      • SH-SY5Y, SH-SY5Y treated with 100 uM Pervanadate, HeLa, HeLa treated with 100 uM Pervanadate, K562, K562 treated with 100 uM Pervanadate
    • 常规说明


      P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA

      BSA is IgG and protease free
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PAK. The final product is generated by affinity chromatography using a PAK 2 derived peptide that is phosphorylated at serine 141.
    • Primary antibody说明

      P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Kinases
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • Structures
      • Focal Adhesions
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases
      • Cancer
      • Signal transduction
      • Protein phosphorylation
      • Serine/threonine kinases
      • Other
      • Neuroscience
      • Processes

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab5247于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/1000. Predicted molecular weight: 65 kDa.
    说明
    WB
    1/1000. Predicted molecular weight: 65 kDa.

    靶标

    • 相关性

      In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
    • 细胞定位

      Cytoplasm. Recruited to focal adhesions upon activation
    • 数据库链接

      • Entrez Gene: 5058 Human
      • Entrez Gene: 5062 Human
      • Entrez Gene: 5063 Human
      • Entrez Gene: 29431 Rat
      • Entrez Gene: 29432 Rat
      • Entrez Gene: 29433 Rat
      • Omim: 300142 Human
      • Omim: 602590 Human
      • Omim: 605022 Human
      • SwissProt: O75914 Human
      • SwissProt: Q13153 Human
      • SwissProt: Q13177 Human
      • SwissProt: Q29502 Rabbit
      • SwissProt: P35465 Rat
      • SwissProt: Q62829 Rat
      • SwissProt: Q64303 Rat
      see all
    • 别名

      • Alpha PAK antibody
      • Beta PAK antibody
      • bPAK antibody
      • CDKN1A antibody
      • Gamma PAK antibody
      • hPAK3 antibody
      • MRX30 antibody
      • MRX47 antibody
      • Oligophrenin 3 antibody
      • OPHN3 antibody
      • P21 (CDKN1A) activated kinase 2 antibody
      • P21 (CDKN1A) activated kinase 3 antibody
      • p21 activated kinase 1 antibody
      • p21 activated kinase 2 antibody
      • p21 activated kinase 3 antibody
      • P21 protein (Cdc42/Rac) activated kinase 1 antibody
      • P21 protein (Cdc42/Rac) activated kinase 2 antibody
      • P21 protein (Cdc42/Rac) activated kinase 3 antibody
      • P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog, yeast) antibody
      • P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
      • P58 antibody
      • P65 PAK antibody
      • p65-PAK antibody
      • PAK 2 antibody
      • PAK 3 antibody
      • PAK1 antibody
      • PAK2 antibody
      • PAK3 antibody
      • PAK3beta antibody
      • PAK65 antibody
      • PAKalpha antibody
      • PAKgamma antibody
      • S6/H4 kinase antibody
      • Serine/threonine protein kinase PAK 1 antibody
      • Serine/threonine protein kinase PAK 2 antibody
      • Serine/threonine protein kinase PAK 3 antibody
      • Serine/threonine protein kinase PAK1 antibody
      • Serine/threonine protein kinase PAK2 antibody
      • Serine/threonine protein kinase PAK3 antibody
      • Serine/threonine-protein kinase PAK 1 antibody
      • STE20 homolog, yeast antibody
      see all

    图片

    • Western blot - Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247)
      Western blot - Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247)
      All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247) at 1/500 dilution

      Lane 1 : SH-SY5Y whole cell extracts
      Lane 2 : SH-SY5Y treated with 100 uM Pervanadate for 1 h
      Lane 3 : HeLa whole cell extract
      Lane 4 : HeLa treated with 100 uM Pervanadate for 1 h
      Lane 5 : K562 whole cell extract
      Lane 6 : K562 treated with 100 uM Pervanadate for 1 h

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution

      Predicted band size: 65 kDa

    实验方案

    • Peptide Competition Experiment
    • Western blotting protocol

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (2)

    发表研究结果有使用 ab5247?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5247 被引用在 2 文献中.

    • Kuželová K  et al. Group I PAK inhibitor IPA-3 induces cell death and affects cell adhesivity to fibronectin in human hematopoietic cells. PLoS One 9:e92560 (2014). WB ; Human . PubMed: 24664099
    • Awasthi A  et al. Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes. J Exp Med 207:1923-38 (2010). WB ; Mouse . PubMed: 20733035

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