Anti-PAK1 + PAK2 + PAK3 (phospho S141)抗体(ab5247)
Key features and details
- Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho S141)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PAK1 + PAK2 + PAK3 (phospho S141)抗体
参阅全部 PAK1 + PAK2 + PAK3 一抗 -
描述
兔多克隆抗体to PAK1 + PAK2 + PAK3 (phospho S141) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat, Rabbit
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免疫原
Synthetic peptide corresponding to Human PAK1 + PAK2 + PAK3 (phospho S141).
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阳性对照
- SH-SY5Y, SH-SY5Y treated with 100 uM Pervanadate, HeLa, HeLa treated with 100 uM Pervanadate, K562, K562 treated with 100 uM Pervanadate
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常规说明
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PAK. The final product is generated by affinity chromatography using a PAK 2 derived peptide that is phosphorylated at serine 141. -
Primary antibody说明
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 65 kDa.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 65 kDa. |
靶标
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相关性
In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death. -
细胞定位
Cytoplasm. Recruited to focal adhesions upon activation -
数据库链接
- Entrez Gene: 5058 Human
- Entrez Gene: 5062 Human
- Entrez Gene: 5063 Human
- Entrez Gene: 29431 Rat
- Entrez Gene: 29432 Rat
- Entrez Gene: 29433 Rat
- Omim: 300142 Human
- Omim: 602590 Human
see all -
别名
- Alpha PAK antibody
- Beta PAK antibody
- bPAK antibody
see all
图片
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Western blot - Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247)All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247) at 1/500 dilution
Lane 1 : SH-SY5Y whole cell extracts
Lane 2 : SH-SY5Y treated with 100 uM Pervanadate for 1 h
Lane 3 : HeLa whole cell extract
Lane 4 : HeLa treated with 100 uM Pervanadate for 1 h
Lane 5 : K562 whole cell extract
Lane 6 : K562 treated with 100 uM Pervanadate for 1 h
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution
Predicted band size: 65 kDa
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab5247 被引用在 2 文献中.
- Kuželová K et al. Group I PAK inhibitor IPA-3 induces cell death and affects cell adhesivity to fibronectin in human hematopoietic cells. PLoS One 9:e92560 (2014). WB ; Human . PubMed: 24664099
- Awasthi A et al. Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes. J Exp Med 207:1923-38 (2010). WB ; Mouse . PubMed: 20733035
