PAI1重组抗体[EPR21850-262]_PAI1抗体(ab317605)| Abcam中文官网

产品名称

Anti-PAI1抗体[EPR21850-262] - BSA and Azide free
参阅全部 PAI1 一抗
描述

兔单克隆抗体[EPR21850-262] to PAI1 - BSA and Azide free
宿主

Rabbit
经测试应用

适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra), IPmore details
种属反应性

与反应: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HUVEC whole cell, A549 whole cell, HepG2 whole cell, SW480 whole cell, Hepa1-6 whole cell, NIH/3T3 whole cell, Human lung cancer tissue, Human placenta tissue, Human liver tissue, Mouse placenta tissue and Rat placenta tissue lysates. IHC-P: Human placenta, Human bladder carcinoma, Mouse placenta and Rat placenta tissues. ICC/IF: HUVEC cells. Flow Cyt (Intra) : HUVEC cells. IP: HepG2 and HUVEC cells.
常规说明
ab317605 is the carrier-free version of ab317604
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C.
存储溶液

pH: 7.2
Constituent: 100% PBS
无载体

是
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR21850-262
同种型

IgG
研究领域

Alternative Versions
- Anti-PAI1 antibody [EPR21850-262] (ab317604)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products

The Abpromise guarantee

Abpromise™承诺保证使用ab317605于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		Use at an assay dependent concentration.
Flow Cyt (Intra)		Use at an assay dependent concentration.
IP		Use at an assay dependent concentration.

说明
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt (Intra) Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

功能

This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
组织特异性

Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
疾病相关

Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
序列相似性

Belongs to the serpin family.
翻译后修饰

Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond.
细胞定位

Secreted.
Target information above from: UniProt accession P05121 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 5054 Human
- Entrez Gene: 18787 Mouse
- Entrez Gene: 24617 Rat
- Omim: 173360 Human
- SwissProt: P05121 Human
- SwissProt: P22777 Mouse
- SwissProt: P20961 Rat
- Unigene: 414795 Human
- Unigene: 713079 Human
- Unigene: 250422 Mouse
- Unigene: 29367 Rat
see all
别名
- Clade E antibody
- Endothelial plasminogen activator inhibitor antibody
- Nexin antibody
- Nexin plasminogen activator inhibitor type 1 antibody
- PAI 1 antibody
- PAI antibody
- PAI-1 antibody
- PAI1_HUMAN antibody
- PLANH1 antibody
- Plasminogen activator inhibitor 1 antibody
- Plasminogen activator inhibitor type 1 antibody
- Serine (or cysteine) proteinase inhibitor antibody
- Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
- Serine proteinase inhibitor clade E member 1 antibody
- serpin antibody
- Serpin E1 antibody
- Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
- Serpin peptidase inhibitor clade E antibody
- Serpine 1 antibody
- SERPINE1 antibody
see all

Western blot - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

All lanes : Anti-PAI1 antibody [EPR21850-262] (ab317604) at 1/1000 dilution

Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 5 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate
Lane 7 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 45 kDa
Observed band size: 45 kDa

This data was developed using ab317604, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: HCT 116 (PMID: 32344898).

Low expression: NIH/3T3 (PMID: 16258002).

The band at approximately 100 kDa may represent PAI-1 in complex with its target protease, t-PA (PMID 21596853, 22069469). This has not been confirmed with additional tests.

The lanes 3-7 were developed using a high sensitivity ECL substrate.

The identity of the lower MW band at approximately 30 kDa (in lanes 5-6) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time: Lanes 1-2: 48 seconds; Lane 3-7: 180 seconds.
Western blot - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

All lanes : Anti-PAI1 antibody [EPR21850-262] (ab317604) at 1/1000 dilution

Lane 1 : Human lung cancer tissue lysate
Lane 2 : Human placenta tissue lysate
Lane 3 : Human liver tissue lysate
Lane 4 : Mouse placenta tissue lysate
Lane 5 : Mouse liver tissue lysate
Lane 6 : Rat placenta tissue lysate
Lane 7 : Rat liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 4-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 45 kDa
Observed band size: 45 kDa

Exposure time: 180 seconds

This data was developed using ab317604, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: liver (PMID: 21898503).

Lanes 1-3 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 4-7 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

This data was developed using ab317604, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PAI1 with ab317604 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human placenta (PMID:18440126). The section was incubated with ab317604 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

This data was developed using ab317604, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling PAI1 with ab317604 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human bladder carcinoma. The section was incubated with ab317604 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

This data was developed using ab317604, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling PAI1 with ab317604 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse placenta. The section was incubated with ab317604 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

This data was developed using ab317604, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat placenta tissue labeling PAI1 with ab317604 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat placenta. The section was incubated with ab317604 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

This data was developed using ab317604, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PAI1 with ab317604 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: almost no staining on human liver. The section was incubated with ab317604 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

This data was developed using ab317604, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PAI1 with ab317604 at 1/500 (0.516 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: no staining on mouse liver. The section was incubated with ab317604 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

This data was developed using ab317604, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling PAI1 with ab317604 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: no staining on rat liver. The section was incubated with ab317604 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

This data was developed using ab317604, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling PAI1 with ab317604 at 1/500 (1.032 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).
Confocal image showing cytoplasmic staining in HUVEC cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control: HCT 116 (PMID: 32344898).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Flow Cytometry (Intracellular) - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

This data was developed using ab317604, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labelling PAI1 with ab317604 at 1/5000 dilution (0.01 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: HCT 116
Immunoprecipitation - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

This data was developed using ab317604, the same antibody clone in a different buffer formulation.
PAI1 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab317604 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317604 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2: ab317604 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317604 in HepG2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds.
Immunoprecipitation - Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

This data was developed using ab317604, the same antibody clone in a different buffer formulation.
PAI1 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab317604 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317604 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2: ab317604 IP in HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317604 in HUVEC whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
The band at approximately100 kDa may represent PAI-1 in complex with its target protease, t-PA (PMID 21596853, 22069469). This has not been confirmed with additional tests.
Anti-PAI1 antibody [EPR21850-262] - BSA and Azide free (ab317605)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

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概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

无载体

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

Datasheet download

Certificate of Compliance

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