Anti-PAI1抗体(ab31280)
Key features and details
- Goat polyclonal to PAI1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PAI1抗体
参阅全部 PAI1 一抗 -
描述
山羊多克隆抗体to PAI1 -
宿主
Goat -
特异性
This antibody is expected to recognise both reported isoforms. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Dog, Chimpanzee -
免疫原
Synthetic peptide corresponding to Human PAI1 aa 50-150 (internal sequence).
Database link: NP_000593.1 -
阳性对照
- IHC-P: Human placenta tissue. WB: Human liver tissue, A549, HepG2, and HeLa cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31280于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
1 hour primary incubation is recommended for this product. |
IHC-P | (1) |
Use a concentration of 4 - 6 µg/ml.
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说明 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 4 - 6 µg/ml. |
靶标
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功能
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
组织特异性
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
疾病相关
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
序列相似性
Belongs to the serpin family. -
翻译后修饰
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 5054 Human
- Omim: 173360 Human
- SwissProt: Q7YSA1 Dog
- SwissProt: P05121 Human
- Unigene: 414795 Human
- Unigene: 713079 Human
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别名
- Clade E antibody
- Endothelial plasminogen activator inhibitor antibody
- Nexin antibody
see all
图片
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All lanes : Anti-PAI1 antibody (ab31280) at 0.1 µg/ml
Lane 1 : A549 cell lysate (35µg protein in RIPA buffer)
Lane 2 : HepG2 cell lysate (35µg protein in RIPA buffer)
Lane 3 : HeLa cell lysate (35µg protein in RIPA buffer)
Developed using the ECL technique.
Predicted band size: 45 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (ab31280)This image is courtesy of an anonymous Abreview
ab31280, at 1/1000, staining plasminogen activator inhibitor 1 (PAI1) in human MDA-MB-231 cells grown in mice by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed and blocked in 10% casein for 30 minutes at 22°C and then incubated with ab31280, for 17 hours at 4°C. Horse polyclonal to goat IgG, diluted 1/200, was used as the secondary antibody.
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Anti-PAI1 antibody (ab31280) at 0.3 µg/ml + Human liver lysate (35µg protein in RIPA buffer)
Developed using the ECL technique.
Predicted band size: 45 kDa -
ab31280 (4µg/ml) staining of paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab31280 被引用在 8 文献中.
- Hogan TB et al. Caveolin-1 peptide regulates p53-microRNA-34a feedback in fibrotic lung fibroblasts. iScience 25:104022 (2022). PubMed: 35330685
- Chen Y et al. MiR145-5p inhibits proliferation of PMVECs via PAI-1 in experimental hepatopulmonary syndrome rat pulmonary microvascular hyperplasia. Biol Open 8:N/A (2019). PubMed: 31649116
- Luo M et al. Circulating miR-30c as a predictive biomarker of type 2 diabetes mellitus with coronary heart disease by regulating PAI-1/VN interactions. Life Sci 239:117092 (2019). PubMed: 31760103
- Tsuge M et al. SK-216, a Novel Inhibitor of Plasminogen Activator Inhibitor-1, Suppresses Lung Metastasis of Human Osteosarcoma. Int J Mol Sci 19:N/A (2018). PubMed: 29510576
- Hirahata M et al. PAI-1, a target gene of miR-143, regulates invasion and metastasis by upregulating MMP-13 expression of human osteosarcoma. Cancer Med 5:892-902 (2016). WB . PubMed: 26817521
- Falkenberg N et al. Secreted uPAR isoform 2 (uPAR7b) is a novel direct target of miR-221. Oncotarget 6:8103-14 (2015). WB . PubMed: 25797271
- Gillespie E et al. Plasminogen activator inhibitor-1 is increased in colonic epithelial cells from patients with colitis-associated cancer. J Crohns Colitis 7:403-11 (2013). PubMed: 22921465
- Keates AC et al. Helicobacter pylori infection stimulates plasminogen activator inhibitor 1 production by gastric epithelial cells. Infect Immun 76:3992-9 (2008). WB ; Human . PubMed: 18519558