Anti-PAF1/PD2 抗体

• WB

Unknown

Western blot - Anti-PAF1/PD2 antibody (AB137519)

7.5 % SDS PAGE

All lanes:

Western blot - Anti-PAF1/PD2 antibody (ab137519) at 1/5000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

H1299 whole cell lysate at 30 µg

Predicted band size: 60 kDa

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• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAF1/PD2 antibody (AB137519)

Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labelling PAF1/PD2 using ab137519 at 1/500.

• WB

Lab

Western blot - Anti-PAF1/PD2 antibody (AB137519)

Lanes 1 - 4 : Merged signal (red and green). Green - ab137519 observed at 80 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab137519 was shown to react with PAF1/PD2 in wild-type HEK-293T cells in Western blot with loss of signal observed at the expected molecular weight in PAF1 CRISPR-Cas9 edited cell line. The band observed at the higher molecular weight in the CRISPR-Cas9 edited sample is likely to represent PAF1 with an insertion. This has not been investigated further and the functional properties of the gene product have not been determined. Wild-type HEK-293T and PAF1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab137519 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PAF1/PD2 antibody (ab137519) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

PAF1 CRISPR-Cas9 Edited HEK-293T cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 60 kDa

Observed band size: 80 kDa

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