重组Anti-PAEP/Glycodelin抗体[EP870Y] (ab53289)
![Western blot - Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289)](https://www.abcam.cn/ps/products/53/ab53289/Images/ab53289-314264-anti-paep-glycodelin-antibody-ep870y-western-blot.jpg "Western blot - Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP870Y] to PAEP/Glycodelin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PAEP/Glycodelin抗体[EP870Y]
参阅全部 PAEP/Glycodelin 一抗 -
描述
兔单克隆抗体[EP870Y] to PAEP/Glycodelin -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human PAEP/Glycodelin aa 150-250 (C terminal). The exact sequence is proprietary.
Database link: P09466 -
阳性对照
- WB: TPA-treated K562 cell and human placenta tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP870Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53289于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
应用说明
Is unsuitable for IHC-P.
靶标
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功能
This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy. -
序列相似性
Belongs to the calycin superfamily. Lipocalin family. -
翻译后修饰
At least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 5047 Human
- Omim: 173310 Human
- SwissProt: P09466 Human
- Unigene: 532325 Human
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别名
- Alpha uterine protein antibody
- gD antibody
- GdA antibody
see all
图片
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Western blot - Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289)All lanes : Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289) at 0.3 µg/ml (purified)
Lane 1 : Human placenta tissue lysate
Lane 2 : Human kidney tissue lysate
Lane 3 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289)](https://www.abcam.cn/ps/products/53/ab53289/Images/ab53289-66078-anti-paep-glycodelin-antibody-ep870y-western-blot.png)
Western blot - Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289)All lanes : Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289) at 1/500 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : TPA-treated K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
![Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289)](https://www.abcam.cn/ps/products/53/ab53289/Images/ab53289-4-benefits-of-recombinant-antibodies.png)
Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab53289 被引用在 4 文献中.
- Cindrova-Davies T et al. Menstrual flow as a non-invasive source of endometrial organoids. Commun Biol 4:651 (2021). PubMed: 34140633
- Turco MY et al. Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium. Nat Cell Biol 19:568-577 (2017). WB ; Human . PubMed: 28394884
- Uysal S et al. Correlation of endometrial glycodelin expression and pregnancy outcome in cases with polycystic ovary syndrome treated with clomiphene citrate plus metformin: a controlled study. Obstet Gynecol Int 2015:278591 (2015). PubMed: 25815012
- Palomino WA et al. A single midcycle dose of levonorgestrel similar to emergency contraceptive does not alter the expression of the L-selectin ligand or molecular markers of endometrial receptivity. Fertil Steril 94:1589-94 (2010). PubMed: 19909947