Name: Anti-PADI4 / PAD4 antibody [OTI4H5]
SKU: ab128086
Rating: 5 (1 reviews)

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Western blot - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

Key features and details

Mouse monoclonal [OTI4H5] to PADI4 / PAD4
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
Reacts with: Human, African green monkey
Isotype: IgG2a

产品名称

Anti-PADI4 / PAD4抗体[OTI4H5]
参阅全部 PADI4 / PAD4 一抗
描述

小鼠单克隆抗体[OTI4H5] to PADI4 / PAD4
宿主

Mouse
经测试应用

适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
种属反应性

与反应: Human, African green monkey
免疫原

Recombinant full length protein, corresponding to amino acids 1-663 of Human PADI4/ PAD4 produced in HEK293T cells (NP_036519).
阳性对照
- HEK293T cells transfected with PADI4 / PAD4 , Human breast, Carcinoma of Human lung, Adenocarcinoma of Human ovary, Human pancreas, Adenocarcinoma of Human endometrium, Human prostate, Carcinoma of Human prostate, Carcinoma of Human bladder and Human lymphoma tissues, COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4 , Hela and Jurkat cells
常规说明

The clone number has been updated from 4H5 to OTI4H5, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol
Concentration information loading...
纯度

Protein A purified
纯化说明

Purified from TCS
克隆

单克隆
克隆编号

OTI4H5
同种型

IgG2a
研究领域

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

The Abpromise guarantee

Abpromise™承诺保证使用ab128086于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/2000. Predicted molecular weight: 74 kDa.
IHC-P	(1)	1/150.
ICC/IF		1/100.
Flow Cyt (Intra)		1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

说明
WB 1/2000. Predicted molecular weight: 74 kDa.
IHC-P 1/150.
ICC/IF 1/100.
Flow Cyt (Intra) 1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

功能

Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
组织特异性

Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
疾病相关

Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
序列相似性

Belongs to the protein arginine deiminase family.
细胞定位

Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
Target information above from: UniProt accession Q9UM07 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 23569 Human
- Omim: 605347 Human
- SwissProt: Q9UM07 Human
- Unigene: 522969 Human
别名
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
- PAD 4 antibody
- PAD antibody
- PADI 4 antibody
- PADI 5 antibody
- PADI H protein antibody
- Padi4 antibody
- PADI4_HUMAN antibody
- PADI5 antibody
- PDI 4 antibody
- PDI 5 antibody
- PDI4 antibody
- PDI5 antibody
- Peptidyl arginine deiminase type IV antibody
- Peptidyl arginine deiminase type V antibody
- Peptidylarginine deiminase IV antibody
- Protein arginine deiminase antibody
- Protein arginine deiminase type 4 antibody
- Protein arginine deiminase type IV antibody
- Protein-arginine deiminase type IV antibody
- Protein-arginine deiminase type-4 antibody
see all

Western blot - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

All lanes : Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086) at 1/2000 dilution

Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PADI4 / PAD4 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 74 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206501)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human breast tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/100, staining COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4 by Immunofluorescence.
Flow Cytometry (Intracellular) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in HeLa cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometry (Intracellular).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded carcinoma of Human lung tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human ovary tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human endometrium tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human prostate tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human bladder tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human lymphoma tissue by Immunohistochemistry.
Flow Cytometry (Intracellular) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in Jurkat cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometry (Intracellular).

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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发表研究结果有使用 ab128086？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab128086 被引用在 36 文献中.

Thomas MA et al. An unbiased proteomic analysis of PAD4 in human monocytes: novel substrates, binding partners and subcellular localizations. Philos Trans R Soc Lond B Biol Sci 378:20220477 (2023). PubMed: 37778379
Wang H et al. Neutrophil Extracellular Traps Aggravate Contrast-Induced Acute Kidney Injury by Damaging Glomeruli and Peritubular Capillaries. J Inflamm Res 16:5629-5646 (2023). PubMed: 38046404
Pasquero S et al. Citrullination profile analysis reveals peptidylarginine deaminase 3 as an HSV-1 target to dampen the activity of candidate antiviral restriction factors. PLoS Pathog 19:e1011849 (2023). PubMed: 38055760
Luo X et al. PAD4-dependent citrullination of nuclear translocation of GSK3β promotes colorectal cancer progression via the degradation of nuclear CDKN1A. Neoplasia 33:100835 (2022). PubMed: 36113195
Gijon M et al. The Clinical and Prognostic Significance of Protein Arginine Deiminases 2 and 4 in Colorectal Cancer. Pathobiology 89:38-48 (2022). PubMed: 34569542

View all Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Human Kidney Tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate based (Vector Laboratories H-3300)

Permeabilization

Yes - 0.2% Triton-X 10 mins RT

Specification

Human Kidney Tissue

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 29 2018

Question

Hello,
I would like to order a reliable pad4 antibody for immunofluorescence. Can you recommend, please, which of the antibodies ab128086 or ab50247 is the best for this application? Also, it is important for me to get the exact concentration of the antibody I'm going to use, please.
Many thanks.

Abcam community

Verified customer

Asked on Jan 18 2013

Answer

Thank you for contacting us.

The exact concentration is necessary when you would like to use Isotype control in IHC or Immunoprecipitation other than that it is OK.

Please note that we can only provide exact concentration for ab128089 because it is purified antibody. We cannot provide concentration for ab50247 because it is a un-purified antibody.

I can recommend buying ab128086.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

回复于 Jan 18 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

纯化说明

克隆

克隆编号

同种型

研究领域

相关产品

Compatible Secondaries

Isotype control

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (36)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PADI4 / PAD4 antibody [OTI4H5]