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Neuroscience Neurology process Growth and Development Neurotrophins

Anti-p75 NGF Receptor抗体[NGFR5] (ab3125)

  • Datasheet
Reviews (5)Q&A (9)References (22)

Product price, shipping and contact information

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
  • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)

Key features and details

  • Mouse monoclonal [NGFR5] to p75 NGF Receptor
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

APC Carrier Free PE PE/Cy7® PerCP/Cy5.5®

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概述

  • 产品名称

    Anti-p75 NGF Receptor抗体[NGFR5]
    参阅全部 p75 NGF Receptor 一抗
  • 描述

    小鼠单克隆抗体[NGFR5] to p75 NGF Receptor
  • 宿主

    Mouse
  • 经测试应用

    适用于: ICC/IF, IHC-Pmore details
    不适用于: WB
  • 种属反应性

    与反应: Rat, Human
    预测可用于: Rabbit, Cat, Baboon不与反应: Mouse
  • 免疫原

    Full length native protein (purified) corresponding to p75 NGF Receptor. Purified from A875 melanoma cell line.

  • 表位

    Amino acids 1 - 160.
  • 阳性对照

    • IHC-P: Human testis tissue. ICC/IF: PC-12 cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Constituents: 0.0268% PBS, 0.2% BSA
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    NGFR5
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Neurotrophins
    • Stem Cells
    • Neural Stem Cells
    • Surface Molecules
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Neuroscience
    • Development

相关产品

  • Alternative Versions

    • PE Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab157333)
    • APC Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab176522)
    • Anti-p75 NGF Receptor antibody [NGFR5] - BSA and Azide free (ab212682)
    • PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5] (ab234270)
    • PerCP/Cy5.5® Anti-p75 NGF Receptor antibody [NGFR5] (ab234446)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3125于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (2)
Use a concentration of 5 µg/ml.
IHC-P (3)
Use a concentration of 1 - 2 µg/ml.
说明
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 1 - 2 µg/ml.
应用说明
Is unsuitable for WB.

靶标

  • 功能

    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • 序列相似性

    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • 结构域

    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • 翻译后修饰

    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession P08138 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 101101519 Cat
    • Entrez Gene: 4804 Human
    • Entrez Gene: 100349095 Rabbit
    • Entrez Gene: 24596 Rat
    • Omim: 162010 Human
    • SwissProt: P08138 Human
    • SwissProt: P07174 Rat
    • Unigene: 415768 Human
    • Unigene: 681726 Human
    • Unigene: 10980 Rat
    see all
  • 别名

    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling p75 NGF Receptor with ab3125 at 2 µg/mL.

  • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
    Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)

    ICC/IF image of ab3125 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

实验方案

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (22)

发表研究结果有使用 ab3125?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3125 被引用在 22 文献中.

  • Zhang Y  et al. Combination of olfactory ensheathing cells and human umbilical cord mesenchymal stem cell-derived exosomes promotes sciatic nerve regeneration. Neural Regen Res 15:1903-1911 (2020). PubMed: 32246639
  • Castle MJ  et al. Postmortem Analysis in a Clinical Trial of AAV2-NGF Gene Therapy for Alzheimer's Disease Identifies a Need for Improved Vector Delivery. Hum Gene Ther 31:415-422 (2020). PubMed: 32126838
  • Brokhman I  et al. Dual embryonic origin of the mammalian enteric nervous system. Dev Biol 445:256-270 (2019). PubMed: 30472119
  • Du Y  et al. Intracellular Notch1 Signaling in Cancer-Associated Fibroblasts Dictates the Plasticity and Stemness of Melanoma Stem/Initiating Cells. Stem Cells 37:865-875 (2019). PubMed: 30941836
  • Rice FL  et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 14 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-p75 NGF Receptor antibody [NGFR5]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (neural precursor)
Permeabilization
No
Specification
neural precursor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 13 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-p75 NGF Receptor antibody [NGFR5]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Spermatogonial stem cells differentiating into gli)
Permeabilization
Yes - 0.1 % Triton-X
Specification
Spermatogonial stem cells differentiating into gli
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dominic Schomberg

Verified customer

提交于 Apr 18 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p75 NGF Receptor antibody [NGFR5]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Bone Marrow)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Bone Marrow
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF, 10% Formic Acid Decalcification
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Premier Laboratory

Verified customer

提交于 Mar 29 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p75 NGF Receptor antibody [NGFR5]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Melanoma)
Specification
Melanoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH9.0
Permeabilization
Yes - 0.25%Triton X-100
Blocking step
DakoCytomation X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Hongwei Shao

Verified customer

提交于 Nov 09 2011

Question

I was wondering if I could get a sample of two antibodies to test this method once before I purchase a large amount of antibody. The two antibodies for which I would like samples are as follows: Anti-Metallothionein antibody [UC1MT] (ab12228) Anti-p75 NGF Receptor antibody [NGFR5] (ab3125).

Read More

Abcam community

Verified customer

Asked on Apr 11 2013

Answer

We do not have any free samples or small samples of any of our antibodies but both ab12228 and ab3125 will be covered by our guarantee for one year if you plan on using them for flow cytometry with samples that are one of the species listed on the datasheets. (Human, ferret, and monkey are listed on the ab3125 datasheet and human, mouse, rat and several others on the ab12228 datasheet).

Note that metallothionein is cytoplasmic, so you will not be able to sort live cells. Antibody ab3125 anti-p75 NGF Receptor, on the other hand, recognizes an extracellular epitope, so you should be able to sort live cells.

Read More

Abcam Scientific Support

回复于 Apr 11 2013

Question

Bonjour,
J'ai utilisé le kit ab64261 pour le NGF (6199) et le TrkA (37837).

pour les photo, nous avons un problème avec notre appareil. Pour le moment cela nous est impossible. C'est pour cela que je voulais vous envoyer les lames.

Salutations

Read More

Abcam community

Verified customer

Asked on May 22 2012

Answer

Merci pour votre émail. Je suis désolée que nous pouvons par regarder les images du coup. Nous sommes en Angleterre, et je pense que ça va devenir compliqué pour envoyer les lames.

Je vais chercher pour trouver des images de mon coté et je vous tiens au courant. Est-ce que en attendant, vous pouvez me confirmerles pointssuivants:

1.)que ces bien des tissus d'origine humaine que vous avez marqué? Ou c'est des tissus d'origine de souris?

2.) C'est quoi comme sérum que vous avez utilisé pour bloquer les lames?

3.) A votre avis, le problème vient de quel réactif?

Je me réjouis de votre réponse. Veuillez s'il vous plait aussi me confirmer comment vous souhaitez procéder.

Read More

Abcam Scientific Support

回复于 May 23 2012

Question

Hi there,I am currently working with Cd271 selected MSCs derived from bone marrow. My experiments now require a Cd271 antibody as well as a CD271 primer for qPCR. I cannot see these products on your website and therefore wondering if you still supply these. I was recommended to try this company by acolleaguebut I'm not having much luck finding these on your site.Any information would be greatly appreciated.

Read More

Abcam community

Verified customer

Asked on Jan 16 2012

Answer

Thank you for your inquiry.

I am pleased to let you know that we currently have a number of CD271 antibodies (24 in total); we sell them under the alternative name 'p75-NGF receptor', such as the rabbit polyclonal antibody ab8874 and the mouse monoclonal ab8877 (as can be seen on each datasheet in the section 'Alternative names'):
Click here (or use the following: https://www.abcam.com/p75-NGF-Receptor-antibody-ab8874.html).
Click here (or use the following: https://www.abcam.com/p75-NGF-Receptor-antibody-ME204-ab8877.html).

These antibodies are guaranteed to work with mouse and human samples (amongst others) and in a number of applications. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (www.abcam.com/Abpromise).

If you are looking for other specifications (e.g. other species or applications), I would be pleased to assist you further in finding the appropriate antibody.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews

We do not have many qPCR primer in our catalog. We have primer for common controls such as GAPDH (ab83588) available, but CD271/p75-NGF receptor is unfortunately not in this selection.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Abcam Scientific Support

回复于 Jan 16 2012

Question

Bonjour, J'ai effectué de nouveaux tests en augmentant la concentration. J'ai obtenu un marquage pour NGF et TrkA fort mais avec beaucoup de fond. La p75 est toujours faible, donc je vais la concentré plus. Pour repondre à votre question, j'ai bien reçu les 3 anticorps dans le même colis. Merci pour votre aide Meilleures salutations

Read More

Abcam community

Verified customer

Asked on Nov 02 2011

Answer

Merci pour ces précisions. N'hésitez pas à nous contacter de nouveau si vous rencontrez des difficultés.  

Read More

Abcam Scientific Support

回复于 Nov 02 2011

Question

Bonjour, J'ai effectué les tests pour les 3 anticorps avec les paramètres que vous m'aviez conseillé. Je n'ai malheureusement pas de resultats très concluant. Je vais effectuer un test en concentrant plus les anticorps. Est ce que le tissu utilisé pour les les tests est le plus apropriez ? Merci d'avance pour votre aide. Meilleures salutations

Read More

Abcam community

Verified customer

Asked on Oct 31 2011

Answer

Le tissu cérébral humain est en effet un tissu de choix pour l'expression de TrkA, NGF et le récepteur p75 NGF. Avez-vous reçu ces 3 anticorps en même temps (même colis de livraison)?  

Read More

Abcam Scientific Support

回复于 Oct 31 2011

Question

Bonjour, nous avons bien reçu vos anticorps, merci. J'ai effectué un premier test qui n'a malheureusement pas fonctionné. Voici les paramètres: NGF (ab 6199): Pas de prétraitement, dilution a 1/500 incubé 30 minute, utilisations du système ENVISION/HRP de Dako pour anticorps rabbit polyclonal, utilisation PBS comme washbuffer. TRKa (ab 37837): Paramètres identique que ci-dessus, dilution à 1/100 P75 (ab 3125): Pré traitement à la chaleur de 3*5 minutes au Micro-ondes à 600w dans tampon citrate a pH 6, dilution à 1/200 incubé pendant 30 minutes, utilisation de système ENVISION/HRP de Dako pour Mouse monoclonal antibody, utiisaion de PBS. J'aimerais savoir si en utilisant de TBS plutôt que du PBS cela fonctionnerait mieux? Et si je dois procéder à un prétraitement pour le NGF et le TrkA ? Le test à été effectué sur du tissus cérébral humain. Merci d'avance pour votre réponse et meilleures salutations.

Read More

Abcam community

Verified customer

Asked on Oct 12 2011

Answer

Merci de nous avoir contactés. Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab3125, ab6199, ab37837 en IHC-P. Notre politique est d'offrir tout d'abord la meilleure assistance technique. Si nous concluons que le produit que vous avez reçu ne fonctionne pas comme mentionné sur sa fiche technique et que ce produit a été commandé sous 6 mois, nous nous ferons un plaisir de vous offrir un remplacement gratuit ou un avoir. J'aimerais donc vous offrir quelques suggestions afin d'optimiser vos résultats. - Pour les 3 anticorps je recommanderais une incubation sur la nuit à 4°C (ou même à température ambiante) en prenant soin que les échantillons ne soient pas sujet à évaporation. Pour les dilutions des anticorps primaires, se reporter aux fiches techniques. - Pour les 3 anticorps je recommanderais également un démasquage de l'antigène à haute température en utilisant du tampon Tris EDTA pH 9,0. Pourriez-vous me communiquer quelques détails concernant la préparation des échantillons (agent et temps de fixation)? Les anticorps secondaires sont-il compatibles? Sont-ils bons? J'espère que ces informations vous aideront. Si les suggestions ne permettent pas d'améliorer vos résultats, n'hésitez pas à me recontacter pour plus d'assistance.

Read More

Abcam Scientific Support

回复于 Oct 12 2011

1-10 of 14 Abreviews or Q&A

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