AB3125

Anti-p75 NGF Receptor抗体[NGFR5]

Name: p75 NGF Receptor抗体[NGFR5] (ab3125)| Abcam中文官网
Brand: Abcam Limited
SKU: AB3125
Rating: 4 (6 reviews)

Anti-p75 NGF Receptor antibody [NGFR5]

(6 Reviews)

(32 Publications)

Mouse Monoclonal p75 NGF Receptor antibody. Suitable for ICC/IF, IHC-P and reacts with Rat, Human samples. Cited in 32 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human NGFR.

查看别名

CD271, TNFRSF16, NGFR, Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, NGF receptor

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (AB3125)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (AB3125)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling p75 NGF Receptor with ab3125 at 2 μg/mL.

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (AB3125)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (AB3125)

ICC/IF image of ab3125 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

不同偶联物与剂型 (5)

Carrier free

Anti-p75 NGF Receptor antibody [NGFR5] - BSA and Azide free
778 PE/Cy7®

PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5]
578 PE

PE Anti-p75 NGF Receptor antibody [NGFR5]
660 APC

APC Anti-p75 NGF Receptor antibody [NGFR5]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-p75 NGF Receptor antibody [NGFR5]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

NGFR5

亚型

IgG1

轻链类型

kappa

不含载体蛋白

反应种属

Rat, Human

应用

ICC/IF, IHC-P

applications

免疫原

Native Full Length Protein corresponding to Human NGFR.

P08138

表位

Amino acids 1 - 160.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Baboon": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

pH: 7.4 Constituents: 0.2% BSA, 0.0268% PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The p75 NGF receptor also known as p75 NGFR or anti-NTR is a transmembrane protein with a molecular mass of approximately 75 kDa. It is a member of the tumor necrosis factor receptor superfamily. This receptor is widely expressed in the nervous system particularly in neurons glial cells and Schwann cells. The p75 receptor primarily binds to neurotrophins such as nerve growth factor (NGF) influencing neuronal survival apoptosis and growth.

Biological function summary

The p75 receptor functions as an important part of a signaling complex that mediates diverse cellular responses. It often forms complexes with other receptors like TrkA but can also signal independently. In particular the p75 protein can form a biological chimera with different co-receptors influencing its downstream effects. This makes it significant in processes like apoptosis and neuronal differentiation as well as influencing neurotrophin-mediated signaling.

Pathways

The p75 receptor plays an important role in pathways involving neuronal survival and apoptosis. One key pathway involves the nerve growth factor (NGF) signaling pathway where p75 collaborates with Trk receptors enhancing or inhibiting NGF signaling based on cellular context. Another pathway is the JNK signaling pathway where the p75 receptor interacts with proteins like sortilin contributing to apoptosis and stress responses in neurons. These interactions determine the outcome of neurotrophic signaling highlighting its role in cell fate decisions.

P75 receptor is associated with several neurodegenerative conditions and cancers. Its involvement in Alzheimer's disease is significant because altered p75 expression and function can contribute to neurodegeneration through apoptosis pathways. This receptor is also linked to glioblastoma where it might promote tumor progression in coordination with proteins such as sortilin. These links make p75 receptor a target of interest for therapeutic intervention in these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed : 24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed : 26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed : 23785138).

See full target information NGFR

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文献 (32)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38791213

2024

Distinctiveness of Femoral and Acetabular Mesenchymal Stem and Progenitor Populations in Patients with Primary and Secondary Hip Osteoarthritis Due to Developmental Dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Mihovil Plečko,Nataša Kovačić,Danka Grčević,Alan Šućur,Andreja Vukasović Barišić,Tea Duvančić,Ivan Bohaček,Domagoj Delimar

JCI insight 9: PubMed38175707

2024

Unbalancing cAMP and Ras/MAPK pathways as a therapeutic strategy for cutaneous neurofibromas.

Applications

Unspecified application

Species

Unspecified reactive species

Helena Mazuelas,Miriam Magallón-Lorenz,Itziar Uriarte-Arrazola,Alejandro Negro,Inma Rosas,Ignacio Blanco,Elisabeth Castellanos,Conxi Lázaro,Bernat Gel,Meritxell Carrió,Eduard Serra

STAR protocols 4:102198 PubMed36976677

2023

Generation of human iPSC-derived neurofibromaspheres for in vitro and in vivo uses.

Applications

Unspecified application

Species

Unspecified reactive species

Helena Mazuelas,Itziar Uriarte-Arrazola,Juana Fernández-Rodríguez,Miriam Magallón-Lorenz,Alberto Villanueva,Conxi Lázaro,Bernat Gel,Eduard Serra,Meritxell Carrió

Frontiers in medicine 9:922347 PubMed35924034

2022

A rare case of arrhythmogenic right ventricular cardiomyopathy associated with mutation: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Wang,Yibing Fang,Dan Zhang,Yifei Li,Shuhua Luo

Nature communications 13:142 PubMed35013231

2022

G-quadruplex DNA structures in human stem cells and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine G Zyner,Angela Simeone,Sean M Flynn,Colm Doyle,Giovanni Marsico,Santosh Adhikari,Guillem Portella,David Tannahill,Shankar Balasubramanian

Chinese medical journal 134:2710-2720 PubMed34845995

2021

Low-intensity extracorporeal shock wave therapy promotes recovery of sciatic nerve injury and the role of mechanical sensitive YAP/TAZ signaling pathway for nerve regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Xi Li,Zhi-Chao Zhang,Jing Peng

Stem cell research & therapy 12:402 PubMed34256823

2021

Potential application of human neural crest-derived nasal turbinate stem cells for the treatment of neuropathology and impaired cognition in models of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Yeon Lim,Sang In Park,Soon A Park,Jung Ho Jeon,Ho Yong Jung,Jung-Min Yon,Sin-Soo Jeun,Hyun Kook Lim,Sung Won Kim

Neural regeneration research 15:1903-1911 PubMed32246639

2020

Combination of olfactory ensheathing cells and human umbilical cord mesenchymal stem cell-derived exosomes promotes sciatic nerve regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhang,Wen-Tao Wang,Chun-Rong Gong,Chao Li,Mei Shi

Human gene therapy 31:415-422 PubMed32126838

2020

Postmortem Analysis in a Clinical Trial of AAV2-NGF Gene Therapy for Alzheimer's Disease Identifies a Need for Improved Vector Delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Castle,Fernando C Baltanás,Imre Kovacs,Alan H Nagahara,David Barba,Mark H Tuszynski

Cells 8: PubMed31569361

2019

Relevance of Neurotrophin Receptors CD271 and TrkC for Prognosis, Migration, and Proliferation in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yannick Foerster,Timo Stöver,Jens Wagenblast,Marc Diensthuber,Sven Balster,Jennis Gabrielpillai,Hannah Petzold,Christin Geissler

View all publications

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Anti-p75 NGF Receptor抗体[NGFR5]