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Anti-p75 NGF Receptor抗体[MC-192] (ab6172)

Reviews (2)Q&A (6)References (13)
Immunocytochemistry - Anti-p75 NGF Receptor antibody [MC-192] (ab6172)

    Key features and details

    • Mouse monoclonal [MC-192] to p75 NGF Receptor
    • Suitable for: ICC
    • Reacts with: Rat
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-p75 NGF Receptor抗体[MC-192]
      参阅全部 p75 NGF Receptor 一抗

    • 描述

      小鼠单克隆抗体[MC-192] to p75 NGF Receptor

    • 宿主

      Mouse

    • 经测试应用

      适用于: ICCmore details

    • 种属反应性

      与反应: Rat

    • 免疫原

      Tissue, cells or virus corresponding to Rat p75 NGF Receptor. PC-12 cells.

    • 阳性对照

      • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.

    • 常规说明


      A well characterised and versatile monoclonal antibody, clone number MC-192, that is of central importance in the study of LANGF (p75-NGF) receptor.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6172于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC
    Use at an assay dependent concentration.
    说明
    ICC
    Use at an assay dependent concentration.

    靶标

    • 功能

      Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.

    • 序列相似性

      Contains 1 death domain.
      Contains 4 TNFR-Cys repeats.

    • 结构域

      Death domain is responsible for interaction with RANBP9.
      The extracellular domain is responsible for interaction with NTRK1.

    • 翻译后修饰

      N- and O-glycosylated.
      O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
      Phosphorylated on serine residues.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD271 antibody
        • CD271 antigen antibody
        • Gp80 LNGFR antibody
        • Gp80-LNGFR antibody
        • Low affinity nerve growth factor receptor antibody
        • Low affinity neurotrophin receptor p75NTR antibody
        • Low-affinity nerve growth factor receptor antibody
        • Nerve growth factor receptor antibody
        • Nerve growth factor receptor TNFR superfamily member 16 antibody
        • NGF receptor antibody
        • Ngfr antibody
        • p75 ICD antibody
        • p75 Neurotrophin receptor antibody
        • p75 NTR antibody
        • p75(NTR) antibody
        • p75NTR antibody
        • TNFR Superfamily Member 16 antibody
        • TNFRSF16 antibody
        • TNR16_HUMAN antibody
        • Tumor necrosis factor receptor superfamily member 16 antibody
        see all

      图片

      • Immunocytochemistry - Anti-p75 NGF Receptor antibody [MC-192] (ab6172)
        Immunocytochemistry - Anti-p75 NGF Receptor antibody [MC-192] (ab6172)

        ab6172 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6172 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      文献 (13)

      发表研究结果有使用 ab6172？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6172 被引用在 13 文献中.

      • Molnár K  et al. Motoneuronal inflammasome activation triggers excessive neuroinflammation and impedes regeneration after sciatic nerve injury. J Neuroinflammation 19:68 (2022). PubMed: 35305649
      • Rogdakis T  et al. Development and Biological Characterization of a Novel Selective TrkA Agonist with Neuroprotective Properties against Amyloid Toxicity. Biomedicines 10:N/A (2022). PubMed: 35327415
      • Niapour N  et al. An efficient system for selection and culture of Schwann cells from adult rat peripheral nerves. Cytotechnology 68:629-36 (2016). PubMed: 25680695
      • Nelson AR  et al. Noradrenergic sympathetic sprouting and cholinergic reinnervation maintains non-amyloidogenic processing of AßPP. J Alzheimers Dis 38:867-79 (2014). IHC ; Rat . PubMed: 24081376
      • Barres BA Designing and troubleshooting immunopanning protocols for purifying neural cells. Cold Spring Harb Protoc 2014:1342-7 (2014). PubMed: 25447277
      View all Publications for this product

      客户评价及客户问答

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      提交评价 提交问题

      1-8 of 8 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p75 NGF Receptor antibody [MC-192]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate sodium
      Sample
      Cat Tissue sections (brain stem)
      Specification
      brain stem
      Permeabilization
      Yes - 0.3% triton
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Sep 12 2014

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p75 NGF Receptor antibody [MC-192]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate sodium
      Sample
      Rat Tissue sections (brainstem slice)
      Specification
      brainstem slice
      Permeabilization
      Yes - 0.3% triton
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Sep 12 2014

      Question

      I have a question about the mouse monoclonal antibody to rat p75NGF Receptor [MC-192] (ab6172).

      Could I please ask you for a reference where it is determined whether this antibody recognizes human P75NGFR?

      Read More
      Answer

      Thank you very much for your interest in ab6172.

      To our knowledge, ab6172 has not been tested in human. Therefore, I can offer a discount off a future purchase if you buy ab6172 now, test it inhuman and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab6172 in human. I will then send a discount code. This code must be issued before purchasing ab6172 so please wait for my reply before ordering.

      2. Purchase ab6172 either by phone, fax, or online (www.abcam.com).

      3. Test it in human.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab6172 turns out to be unsuitable for human, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Question

      I purchased a vial of LANGF (ab6172). However, while trying to run the staining following your recommendations (1-2 micro grams per ml), I noticed that there is no concentration given for the vial. Thus it is difficult to run the experiment. No-one in the UK or US seem to know the concentration which is a little disturbing and I have been advised to e-mail you. I would be grateful if you could let me know the concentration as soon as impossible as we are working to a deadline and our first batch of slides are now defunct.

      Read More
      Answer

      Thank you for your enquiry. I apologise for any confusion that has been caused with product Ab6172. There is a concentration value cited on the product data sheet, as 1.0mg/ml. You should have received a copy of this data sheet upon receipt of the product. I apologise if this was not the case, it is possible that the data sheet has only been recently updated with this information. Good luck with your research, if you need any further clarification please do not hesitate to get back in touch with me.

      Read More

      Question

      Can this antibody be used for human cells?

      Read More
      Answer

      Dear Dr Hilker I am afraid that this antibody only recognises rat Low Affinity Nerve Growth Factor receptor. Regards Michael

      Read More

      Question

      would it be possible to obtain this antibody biotinilated. We will by a very big amount of it. What could be the price? Thanks

      Read More
      Answer

      Thank you for your enquiry. This antibody is not currently available biotinylated, however we do offer a conjugation service for £150 pounds sterling or $225 US dollars. We would need to biotinylate at least 1mg antibody; the price listed on our web site (£187.50 pounds sterling or $282 US dollars) is for 0.1mg unlabelled antibody, so the total price of 1mg biotinylated antibody would be £2025 pounds sterling or $3045 US dollars.

      Read More

      Question

      Could this antibody be used for FACS analysis of PC12 cells?

      Read More
      Answer

      Yes, this antibody is ideal for FACS of PC12 cells.

      Read More

      Question

      Could this antibody be used formalin fixed and paraffin section ?

      Read More
      Answer

      Yes, the antibody is excellent in these applications.

      Read More

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