Anti-p73抗体(ab14430)
Key features and details
- Rabbit polyclonal to p73
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-p73抗体
参阅全部 p73 一抗 -
描述
兔多克隆抗体to p73 -
宿主
Rabbit -
特异性
The antibody is not expected to react with deltaNp73. -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chimpanzee, Rhesus monkey, Gorilla, African green monkey, Orangutan
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免疫原
Synthetic peptide corresponding to Human p73 aa 1-62. The epitope is found in isoforms alpha, beta, gamma, delta, epsilon, zeta, and kappa of human p73.
Database link: O15350 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antibody was purified using an epitope specific to p73 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14430于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use a concentration of 0.1 µg/ml.
|
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| WB | (2) |
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 69 kDa.
|
| 说明 |
|---|
|
IP
Use a concentration of 0.1 µg/ml. |
|
WB
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 69 kDa. |
靶标
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功能
Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. -
组织特异性
Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. -
序列相似性
Belongs to the p53 family.
Contains 1 SAM (sterile alpha motif) domain. -
结构域
Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
The WW-binding motif mediates interaction with WWOX. -
翻译后修饰
Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
Ubiquitinated; leading to its degradation by the proteasome. -
细胞定位
Nucleus. Accumulates in the nucleus in response to DNA damage. - Information by UniProt
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数据库链接
- Entrez Gene: 7161 Human
- Entrez Gene: 22062 Mouse
- Omim: 601990 Human
- SwissProt: O15350 Human
- SwissProt: Q9JJP2 Mouse
- Unigene: 697294 Human
- Unigene: 706990 Human
- Unigene: 78015 Mouse
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别名
- p53 like transcription factor antibody
- p53 related protein antibody
- p53-like transcription factor antibody
see all
图片
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Western blot - Anti-p73 antibody (ab14430)All lanes : Anti-p73 antibody (ab14430) at 0.1 µg/ml
Lane 1 : HEK293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : RKO cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 69 kDa
Exposure time: 75 seconds -
Immunoprecipitation - Anti-p73 antibody (ab14430)Immunoprecipitation of p73 with ab14430 at 0.1 µg/mL.
Lane 1 and 2: Different lots of ab14430 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.Lane 2: IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Detection: Chemiluminescence
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab14430 被引用在 22 文献中.
- Pallavicini G et al. Lestaurtinib inhibits Citron kinase activity and medulloblastoma growth through induction of DNA damage, apoptosis and cytokinesis failure. Front Oncol 13:1202585 (2023). PubMed: 37404750
- Uboveja A et al. p73-regulated FER1L4 lncRNA sponges the oncogenic potential of miR-1273g-3p and aids in the suppression of colorectal cancer metastasis. iScience 25:103811 (2022). PubMed: 35198876
- Iegiani G et al. CENPE Inhibition Leads to Mitotic Catastrophe and DNA Damage in Medulloblastoma Cells. Cancers (Basel) 13:N/A (2021). PubMed: 33804489
- Raghubir M et al. Riluzole-induced apoptosis in osteosarcoma is mediated through Yes-associated protein upon phosphorylation by c-Abl Kinase. Sci Rep 11:20974 (2021). PubMed: 34697383
- Cai BH et al. Synergistic activation of the NEU4 promoter by p73 and AP2 in colon cancer cells. Sci Rep 9:950 (2019). PubMed: 30700826