Anti-P70 S6 Kinase (phospho T389)抗体(ab2571)
Key features and details
- Rabbit polyclonal to P70 S6 Kinase (phospho T389)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-P70 S6 Kinase (phospho T389)抗体
参阅全部 P70 S6 Kinase 一抗 -
描述
兔多克隆抗体to P70 S6 Kinase (phospho T389) -
宿主
Rabbit -
特异性
Phospho-p70 S6 Kinase (Thr389/412) Ab detects endogenous levels of p70 S6 Kinase only when phosphorylated at Threonine 389/412
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经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human P70 S6 Kinase (phospho T389).
Database link: P23443 -
常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 3505 Phospho-p70 S6 Kinase Antibody. 3505-100 is the same size as the 100 µl size of ab2571.
p70 S6 Kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. P70 S6 Kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5’oligopyrimidine tract mRNAs. A second isoform, p85 S6 Kinase, is derived from the same gene and is identical to p70 S6 Kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 0.877% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
纯度
Affinity purified -
Primary antibody说明
p70 S6 Kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. P70 S6 Kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5’oligopyrimidine tract mRNAs. A second isoform, p85 S6 Kinase, is derived from the same gene and is identical to p70 S6 Kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/500.
The optimal conditions should be determined individually. |
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (4) |
Use a concentration of 1 - 2 µg/ml. This antibody detects bands at approximately 70 and 85 kDa.
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| 说明 |
|---|
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ICC/IF
1/100 - 1/500. The optimal conditions should be determined individually. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 - 2 µg/ml. This antibody detects bands at approximately 70 and 85 kDa. |
靶标
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相关性
The protein p70 S6 kinase is critical for cell cycle progression and cell survival. In response to mitogen stimulation, p70 S6 kinase activation up-regulates ribosomal biosynthesis and enhances the translational capacity. The p70S6K phosphorylates the S6 protein of the 40S subunit of the ribosome. This kinase was first characterized as an insulin/mitogen-activated protein kinase, whose major known substrate is the 40 S ribosomal subunit protein S6. The p70 S6 kinase is activated by diverse stimuli through a multi-site phosphorylation such as Thr-252 and Ser-434. In Alzheimer's Disease (AD), p70 S6 kinase activation is associated with PHF-tau (hyperphosphorylated tau) accumulation. In non-neuronal cells, p70 S6 kinase have been shown to regulate actin polymerization. -
细胞定位
Cell junction › synapse › synaptosome By similarity. Mitochondrion outer membrane. Mitochondrion. Note: Colocalizes with URI1 at mitochondrion. Isoform Alpha I: Nucleus. Cytoplasm Isoform Alpha II: Cytoplasm -
数据库链接
- Entrez Gene: 6198 Human
- Entrez Gene: 72508 Mouse
- Entrez Gene: 83840 Rat
- Omim: 608938 Human
- SwissProt: P23443 Human
- SwissProt: Q8BSK8 Mouse
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别名
- 70 kDa ribosomal protein S6 kinase 1 antibody
- p70 alpha antibody
- p70 S6 kinase alpha antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
Immunohistochemistry analysis of human liver cancer tissue sections staining Phospho-p70 S6 Kinase using ab2571 at 1/200. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
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Western blot - Anti-S6K1 (phospho T389) antibody (ab2571)This image is courtesy of an anonymous AbreviewAll lanes : Anti-P70 S6 Kinase (phospho T389) antibody (ab2571) at 1/250 dilution
Lane 1 : Normal Rat liver whole tissue lysate.
Lane 2 : Rat liver whole tissue lysate 24 hours after 90% partial hepatectomy treated with AEE788 (50 mg/kg).
Lane 3 : Rat liver whole tissue lysate 1 week after 90% partial hepatectomy treated with AEE788 (50 mg/kg).
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 70 kDa why is the actual band size different from the predicted?
Blocking Step: 5% Milk for 1 hour at 25°C. -
Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)Western blot analysis of p70 S6 Kinase phosphorylation expression in Insulin treated
Jurkat whole cell lysates. The lane on the left is treated with the antigen-specific peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)Immunofluorescence analysis of Phospho-p70 S6 Kinase in 293 cells
数据表及文件
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SDS download
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Datasheet download
文献 (50)
ab2571 被引用在 50 文献中.
- Zhang L et al. Comparison of the Effects of Inorganic or Amino Acid-Chelated Zinc on Mouse Myoblast Growth in vitro and Growth Performance and Carcass Traits in Growing-Finishing Pigs. Front Nutr 9:857393 (2022). PubMed: 35464034
- Jiang W et al. RagC GTPase regulates mTOR to promote chemoresistance in senescence-like HepG2 cells. Front Physiol 13:949737 (2022). PubMed: 36267578
- Li Y et al. Regulation of the mammalian maternal-to-embryonic transition by eukaryotic translation initiation factor 4E. Development 148:N/A (2021). PubMed: 34013332
- Xia T et al. ColXV Aggravates Adipocyte Apoptosis by Facilitating Abnormal Extracellular Matrix Remodeling in Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32024006
- Matsumoto N et al. PP1C and PP2A are p70S6K Phosphatases Whose Inhibition Ameliorates HLD12-Associated Inhibition of Oligodendroglial Cell Morphological Differentiation. Biomedicines 8:N/A (2020). PubMed: 32316234