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Cell Biology Cell Cycle Cell Cycle Inhibitors Rb

Anti-P70 S6 Kinase (phospho T389)抗体(ab2571)

  • Datasheet
  • SDS
Reviews (5)Q&A (7)References (47)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
  • Western blot - Anti-S6K1 (phospho T389) antibody (ab2571)
  • Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
  • Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)

Key features and details

  • Rabbit polyclonal to P70 S6 Kinase (phospho T389)
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-P70 S6 Kinase (phospho T389)抗体
  • 描述

    兔多克隆抗体to P70 S6 Kinase (phospho T389)
  • 宿主

    Rabbit
  • 特异性

    Phospho-p70 S6 Kinase (Thr389/412) Ab detects endogenous levels of p70 S6 Kinase only when phosphorylated at Threonine 389/412

  • 经测试应用

    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human P70 S6 Kinase (phospho T389).
    Database link: P23443

  • 常规说明

    This product is manufactured by BioVision, an Abcam company and was previously called 3505 Phospho-p70 S6 Kinase Antibody. 3505-100 is the same size as the 100 µl size of ab2571.

    p70 S6 Kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. P70 S6 Kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5’oligopyrimidine tract mRNAs. A second isoform, p85 S6 Kinase, is derived from the same gene and is identical to p70 S6 Kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 0.877% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS
  • Concentration information loading...
  • 纯度

    Affinity purified
  • Primary antibody说明

    p70 S6 Kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. P70 S6 Kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5’oligopyrimidine tract mRNAs. A second isoform, p85 S6 Kinase, is derived from the same gene and is identical to p70 S6 Kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • S6K Family
    • Metabolism
    • Types of disease
    • Obesity

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2571于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100 - 1/500.

The optimal conditions should be determined individually.

IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (4)
Use a concentration of 1 - 2 µg/ml. This antibody detects bands at approximately 70 and 85 kDa.
说明
ICC/IF
1/100 - 1/500.

The optimal conditions should be determined individually.

IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 2 µg/ml. This antibody detects bands at approximately 70 and 85 kDa.

靶标

  • 相关性

    The protein p70 S6 kinase is critical for cell cycle progression and cell survival. In response to mitogen stimulation, p70 S6 kinase activation up-regulates ribosomal biosynthesis and enhances the translational capacity. The p70S6K phosphorylates the S6 protein of the 40S subunit of the ribosome. This kinase was first characterized as an insulin/mitogen-activated protein kinase, whose major known substrate is the 40 S ribosomal subunit protein S6. The p70 S6 kinase is activated by diverse stimuli through a multi-site phosphorylation such as Thr-252 and Ser-434. In Alzheimer's Disease (AD), p70 S6 kinase activation is associated with PHF-tau (hyperphosphorylated tau) accumulation. In non-neuronal cells, p70 S6 kinase have been shown to regulate actin polymerization.
  • 细胞定位

    Cell junction › synapse › synaptosome By similarity. Mitochondrion outer membrane. Mitochondrion. Note: Colocalizes with URI1 at mitochondrion. Isoform Alpha I: Nucleus. Cytoplasm Isoform Alpha II: Cytoplasm
  • 数据库链接

    • Entrez Gene: 6198 Human
    • Entrez Gene: 72508 Mouse
    • Entrez Gene: 83840 Rat
    • Omim: 608938 Human
    • SwissProt: P23443 Human
    • SwissProt: Q8BSK8 Mouse
    • 别名

      • 70 kDa ribosomal protein S6 kinase 1 antibody
      • p70 alpha antibody
      • p70 S6 kinase alpha antibody
      • p70 S6K antibody
      • p70 S6KA antibody
      • p70(S6K) alpha antibody
      • p70s6kA antibody
      • PS6K antibody
      • Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
      • ribosomal protein S6 kinase beta 1 antibody
      • ribosomal protein S6 kinase I antibody
      • Ribosomal protein S6 kinase, 70-kD antibody
      • RPS6KB1 antibody
      • S6K antibody
      • S6K1 antibody
      • Serine/threonine kinase 14 alpha antibody
      • Serine/threonine protein kinase 14A antibody
      • STK14A antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)

      Immunohistochemistry analysis of human liver cancer tissue sections staining Phospho-p70 S6 Kinase using ab2571 at 1/200. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

    • Western blot - Anti-S6K1 (phospho T389) antibody (ab2571)
      Western blot - Anti-S6K1 (phospho T389) antibody (ab2571)This image is courtesy of an anonymous Abreview
      All lanes : Anti-P70 S6 Kinase (phospho T389) antibody (ab2571) at 1/250 dilution

      Lane 1 : Normal Rat liver whole tissue lysate.
      Lane 2 : Rat liver whole tissue lysate 24 hours after 90% partial hepatectomy treated with AEE788 (50 mg/kg).
      Lane 3 : Rat liver whole tissue lysate 1 week after 90% partial hepatectomy treated with AEE788 (50 mg/kg).

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 70 kDa why is the actual band size different from the predicted?



      Blocking Step: 5% Milk for 1 hour at 25°C.

      See Abreview

    • Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
      Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)

      Western blot analysis of p70 S6 Kinase phosphorylation expression in Insulin treated
      Jurkat whole cell lysates. The lane on the left is treated with the antigen-specific peptide.

    • Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)
      Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase (phospho T389) antibody (ab2571)

      Immunofluorescence analysis of Phospho-p70 S6 Kinase in 293 cells

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (47)

    发表研究结果有使用 ab2571?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2571 被引用在 47 文献中.

    • Xia T  et al. ColXV Aggravates Adipocyte Apoptosis by Facilitating Abnormal Extracellular Matrix Remodeling in Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32024006
    • Matsumoto N  et al. PP1C and PP2A are p70S6K Phosphatases Whose Inhibition Ameliorates HLD12-Associated Inhibition of Oligodendroglial Cell Morphological Differentiation. Biomedicines 8:N/A (2020). PubMed: 32316234
    • Wang X  et al. TFEB-NF-?B inflammatory signaling axis: a novel therapeutic pathway of Dihydrotanshinone I in doxorubicin-induced cardiotoxicity. J Exp Clin Cancer Res 39:93 (2020). PubMed: 32448281
    • Wang Y  et al. Propofol Attenuates a-Synuclein Aggregation and Neuronal Damage in a Mouse Model of Ischemic Stroke. Neurosci Bull 36:289-298 (2020). PubMed: 31520398
    • Chen G  et al. Cardiomyocyte-derived small extracellular vesicles can signal eNOS activation in cardiac microvascular endothelial cells to protect against Ischemia/Reperfusion injury. Theranostics 10:11754-11774 (2020). PubMed: 33052245
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 12 Abreviews or Q&A

    Western blot abreview for Anti-S6K1 (phospho T389) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Liver)
    Loading amount
    30 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 02 2012

    Western blot abreview for Anti-S6K1 (phospho T389) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Liver)
    Loading amount
    50 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 13 2012

    Western blot abreview for Anti-S6K1 (phospho T389) antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Liver)
    Loading amount
    50 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 13 2012

    Western blot abreview for Anti-S6K1 (phospho T389) antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver)
    Loading amount
    20 µg
    Specification
    liver
    Treatment
    AEE788 50mg/kg 24h and 1week
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 19 2010

    Question

    The samples are prepared from schwann cells and DRG neurons and pancreatic cancer cell lines (one human, one rat origin, one mouse origin ) we chose the antibodies according to the reactivity mentioned on the abcam data sheet and the lysates were prepared using a non denaturing lysis buffer, aliquoted and stored at -80 degrees centigrade. I use 10% and 12.5% acrylamide gels depending on the molecular weight of the protein. initially 40 ug protein was loaded and the gels were run at 70 volts and transfered on to PVDF membrane (ice cold). All buffers were prepared as suggested in the western blot guide by Abcam. 5% milk was used to block the membrane, washed thrice with TBST and/ or TBS. Incubated with respective antibodies (initially 1: 1000 dilution) overnight in cold room with agitation. The blots were added with secondary antibody the following day (secondary antibody from abcam (ab97023 and ab97051 which were aliquoted and stored at -20 degrees centigrade) incubated for 1 hour at room temperature and then washed thrice with TBS/and or TBST. probed with chemiluminescent solution from Biorad. Bands were not detected. We tried loading 50ug protein (we cannot load more owing to the limited volumes of sample we have and the nature of the project) and then tried using 1:500 and 1:250 dilutions, but still the antibodies were not working. We have been using this protocol and works fine with all the other abcam antibodies. Please let me know if I need to elaborate on any of these steps.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 24 2013

    Answer


    Upon reviewing your protocol, it is troubling to me that all 4 of these antibodies are not working. While it is conceivable that all four antibodies are not performing properly, typically when multiple antibodies are not working it is an issue with sample preparation, membrane transfer, or the detection substrate. In your case, I think the following troubleshooting steps are the most worth your while attempting, with tip 1 being the first thing you try since it is the most likely to make the targets recognizable by the antibodies:
    1. You mentioned your samples were non-denatured. ab2571, ab32124, and ab81212 were all raised against synthetic peptides and will require linear proteins in order to recognize their epitopes. Thus, your samples should be both reduced and denatured (SDS and beta-mercaptoethanol or DTT) and boiled for 5 minutes at 100C prior to freezing at -20C. RIPA buffer is a good choice for mitochondrial and membrane proteins such as these.
    2. To enrich the target proteins, have you considered a mitochondria isolation kit such as ab110170 (https://www.abcam.com/Mitochondria-Isolation-Kit-for-Cultured-Cells-ab110170.html)?
    3. Have you insured that the proteins are being transferred with a reversible stain such as Ponceau S? Since you are using PVDF, make sure to pre-soak the membrane in MeOH, then in transfer buffer.
    4. If you are including milk in your antibody dilutions try removing milk or reducing to 0.5% milk for the antibody dilutions only.
    Further troubleshooting tips, including no signal specific tips, are included through the link below:
    https://www.abcam.com/index.html?pageconfig=resource&rid=11352
    Please let me know the results of troubleshooting, especially step 1. I look forward to your reply and sincerely hope this information helps.

    Read More

    Abcam Scientific Support

    回复于 Jan 24 2013

    Question

    I would like to request MSDS for the following products:
    ab51044
    ab47367
    ab2571
    ab79853
    ab76165
    ab31232
    Thank you in advance for your help!

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2012

    Answer

    Thank you for contacting Abcam.



    Attached please find the MSDS you requested. Please do not hesitate to contact me if you have any additional questions.

    Read More

    Abcam Scientific Support

    回复于 Sep 06 2012

    Question

    Hi,
    With regards to your email and previous discussions we wish to purchase the following antibodies free of charge
    Anti-smad2 (ps467) #ab53100 £234
    Anti-IKB (phosphor s32 + s36) ab 39a1431 #ab12135 £234
    What is the best way to proceed with regards to ordering thse items? In addition, I know you have mentioned previously about another p-p70 s6 kinase we could use, could you possible provide me with this information again?
    Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Jun 27 2012

    Answer

    Thank you for getting back to me.
    I can set up the order for the anti-smad2 (ps467) (ab53100) and anti-IKB (phospho s32 + s36) (ab12135) directly for you. Could you please just confirm that the delivery address I have for you is correct:
    xxxxxxxx

    Once this has been confirmed I will organise for the delivery.
    The main alternative antibody we discussed directed against the phospho-T389 S6K was ab2571. Each new batch of this antibody is tested for its specificity by observing the change in band detected when a sample of HeLa cells are treated with and without l-phosphatase. As presented on the datasheet of this antibody, it has also been used with AEE788 treated rat samples indicating it shows strong phospho-specificity.
    I hope this information has been of help. If you have any further questions, please do not hesitate to ask.
    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    回复于 Jun 27 2012

    Question

    Vielen Dank für die schnelle Hilfe.Ich hoffe, ich komme weiter.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 26 2012

    Answer

    Lassen Sie uns auf jeden Fall wissen, wie Sie vorankommen!

    Sollten die Vorschläge nämlich nicht zum Erfolg führen, werden wir Ihnen einen Ersatz (mit einem primären Antikörper Ihrer Wahl) oder eine Gutschrift schicken.

    Ich drücke Ihnen aber die Daumen, dass es klappt und Sie die Ergebnisse bekommen, die Sie erwarten!

    Read More

    Abcam Scientific Support

    回复于 Apr 26 2012

    Question

    1) Abcam product code ab 2571
    2) Abcam order reference number or product batch number x
    3) Description of the problem
    Ich habe den Anti-S6K(phospho-T389)antibody (ab2571) in einer 1/500
    Verdünnung gelöst in 5% BSA für den Westernblot genutzt, um
    nachzuweisen, dass die phosphorylierte Form von S6K unter Nutzung von
    Rapamycin gehemmt wird und somit vermindert. In meinen Versuchen habe
    ich die humane Zellinie X benutzt und diese für
    jeweils 24h mit 100nM und 10nM Rapamycin inkubiert. Eine
    Negativkontrolle habe ich auch mitgeführt.
    Nun müsste der Antikörper bei ca.70kDa binden, in meinem WB, den ich
    Ihnen im Anhang beigefügt habe, sieht man bei 70kDa gar keine Banden,
    dafür starke Banden bei 40,35kDa und 10kDa. Woran könnte dies liegen?
    Auch hat das BSA an den Marker gebunden. Können Sie mir eine
    Positivkotrolle für den Antikörper empfehlen bzw. mitschicken?
    4) Sample preparation:
    Type of sample (whole cell lysates, fraction, recombinant protein?): Zelllysat
    Lysis buffer: Tris Base, Glycerol,SDS
    Protease inhibitors: nein
    Phosphatase inhibitors nein
    Reducing agent nein
    Boiling for ?5 min? ja mit Trypanblau,Glycerol, Puffer
    Protein loaded 15 ug/lane or cells/lane
    Positive control: nein
    Negative contro:ja
    5) Percentage of gel 10%
    Type of membrane PVDF Transfer Membran
    Protein transfer verified ja
    Blocking agent and concentration: 5% BSA
    Blocking time 30min
    Blocking temperature Raumtemperatur
    6) Primary antibody (If more than one was used, describe in
    ?additional notes?) : Anti-S6K(phospho-T389)antibody (ab2571)
    Concentration or dilution 10µl in 5ml 5%BSA
    Diluent buffer 5% BSA in TBS-T
    Incubation time über Nacht
    Incubation temperature: 4°C
    7) Secondary antibody:HRP-anti-rabbit
    Species: Kaninchen
    Reacts against: Kaninchen
    Concentration or dilution 1:5000
    Diluent buffer 5% BSA in TBS-T
    Incubation time: 2h
    Incubation temperature: Raumtemperatur
    Fluorochrome or enzyme conjugate: ja
    8) Washing after primary and secondary antibodies:
    Buffer: TBS-T
    Number of washes: 3×10min
    9)Detection method: Reagenz Dura Pierce
    10) How many times have you run this staining? Ich habe den Versuch
    jetzt 3 Mal durchgeführt und immer unspezifische Banden bekommen.
    Einmal habe ich den Antikörper auch in 5% Milch gelöst und die Membran
    1h bei Raumtemperatur in Milch geblockt. Das Ergebnis war schlechter,
    das Bild ist Ihnen beigefügt.
    Do you obtain the same results every time? ja
    What steps have you altered to try and optimize the use of this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

    Es tut mir leid, dass Sie Probleme mit unserem S6K-Antikörper hatten.

    Die Ergebnisse mit Milch-Blockpuffer sind wirklich schlechter als für den BSA-Block; damit haben Sie also schon einen wichtigen Schritt optimiert.

    Bei der Durchsicht Ihres Protokolles ist mir jedoch aufgefallen, dass Sie keine Protease- oder Phosphataseinhibitoren verwenden. Proteolyse und Dephosphorylierung sind jedoch Prozesse, die sofort einsetzen, sobald die Zellaufarbeitung beginnt. Es könnte daher gut möglich sein, dass Sie aus diesem Grund keine spezifischen Banden für das Phosphoprotein detektieren, da der Antikörper nur phosphoryliertes Protein bindet. Wir würden Ihnen empfehlen, Protease- und Phosphataseinhibitoren wie Natriumfluorid oder Natriumorthovanadat zu verwenden. Für Details hierzu habe ich unser Protokoll angehangen, das Sie auch auf unserer Webseite finden können (https://www.abcam.com/index.html?pageconfig=resource&rid=11375).

    Die unspezifischen Banden könnten auch auf den Sekundärantikörper zurückzuführen sein. Mit einer no-primary-Kontrolle kann dies leicht ermittelt werden.

    Als Positivkontrolle für die phosphorylierte S6-Kinase könnten wir Ihnen folgendes empfehlen:

    Liver (Rat) Whole Cell Lysate - normal tissue (ab29413) (siehe Datenblatt ab2571)
    HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate - TNF alpha stimulated (ab14658)
    Breast (Human) Tissue Lysate - Ductal Carcinoma (ab43360)
    Jurkat cells treated with insuline

    Diese Lysate haben wir in unserem Katalog verfügbar. Allerdings handelt es sich dabei auch um gängige Zelllinien oder Lysate, die Sie eventuellselbst im Labor haben (oder von einem Nachbarlabor bekommen können). Außerdem würde ich erwarten, dass Sie mit den humanen Zellen ein Signal bekommen sollten, wenn Sie die Inhibitoren dem Lysepuffer zusetzen. (Ich möchte Sie also nicht zum Kauf eines dieser Produkte drängen.)

    Sollten meine Vorschläge nicht zum Erfolg führen, werde ich Ihnen gerne einen Ersatz oder eine Gutschrift schicken, da dann der Antikörper vermutlich nicht richtig funktioniert (d.h. Ihr Protokoll ist sonst vollkommen in Ordnung).

    Vielen Dank für Ihre Kooperation und Hilfsbereitschaft. Bitte lassen Sie mich wissen, ob ich Ihnen helfen konnte und zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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    Abcam Scientific Support

    回复于 Apr 24 2012

    Question

    phospho T389 S6K-AK für IHC und WB

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    Abcam community

    Verified customer

    Asked on Mar 21 2012

    Answer

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Unserem Wissen nach wurde ab2571 bisher noch nicht in IHC getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab2571 abdecken, und gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden.

    Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

    1.) Bestätigen Sie mir bitte, dass Sie ab2571 inIHC testen möchten.

    2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

    3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

    4.) Testen Sie den Antikörper in IHC.

    5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
    Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
    https://www.abcam.com/abreviews

    6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderenprimären Antikörperzu dem gleichen Preis wie ab2571 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab2571 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

    Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

    Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
    https://www.abcam.com/collaborationdiscount

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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    Abcam Scientific Support

    回复于 Mar 21 2012

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