AB109393

重组Anti-P70 S6 Kinase beta/SRK (phospho S371)抗体[EPR2132]

Anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(20 Publications)

Rabbit Recombinant Monoclonal P70 S6 Kinase beta/SRK phospho S371 antibody. Suitable for WB and reacts with Human samples. Cited in 20 publications.

查看别名

STK14B, RPS6KB2, Ribosomal protein S6 kinase beta-2, S6K-beta-2, S6K2, 70 kDa ribosomal protein S6 kinase 2, S6 kinase-related kinase, Serine/threonine-protein kinase 14B, p70 ribosomal S6 kinase beta, P70S6K2, p70-S6K 2, SRK, S6K-beta, p70 S6 kinase beta, p70 S6K-beta, p70 S6KB, p70-beta

2 Images

Unknown

Western blot - Anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132] (AB109393)

All lanes:

Western blot - Anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132] (ab109393) at 1/1000 dilution

Lane 1:

293T cell lysate, untreated at 10 µg

Lane 2:

293T cell lysate, treated with Alkaline Phosphatase (AP) at 10 µg

Predicted band size: 53 kDa

false

CiteAb

Western blot - Anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132] (AB109393)

P70 S6 Kinase beta/SRK (phospho S371) western blot using anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132] ab109393. Publication image and figure legend from Wang, W. Y., Cao, Y. X., et al., 2019, Cancer Cell Int, PubMed 30996686.

ab109393 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109393 please see the product overview.

Silencing of ST6GALNAC1 inhibits OCSCs stemness. a Protein bands of Akt, p-Akt, PI3K, p-PI3K, S6, p-S6 and β-catenin proteins by western blot analysis; b protein expression of Akt, p-Akt, PI3K, S6, p-S6 and β-catenin by western blot analysis; The measurement data were expressed as mean ± standard deviation and analyzed by t-test. *p < 0.05; the experiment was repeated 3 times

false

不同偶联物与剂型 (1)

Carrier free

Anti-P70 S6 Kinase beta/SRK (phospho S371) antibody [EPR2132] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2132

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody only detects p70 S6 Kinase phosphorylated at serine 371.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

P70 S6 Kinase beta also known as SRK is a protein kinase involved in the regulation of cellular growth and metabolism. It has a molecular mass of approximately 70 kDa. This kinase is predominantly expressed in brain liver and muscle tissues. P70 is an important mediator in the process of protein translation acting mechanically by phosphorylating the ribosomal protein S6 which in turn enhances the translation of a subset of mRNAs related to cell growth processes.

Biological function summary

P70 S6 Kinase beta/SRK is a significant component in the cellular signaling complexes that manage cell size and energy regulation. It participates in the mechanistic target of rapamycin (mTOR) pathway. Through its enzymatic activity it helps cells respond to growth factors and nutrients. By motivating the translation of mRNAs p70 sustains ribosome biogenesis and cell growth.

Pathways

P70 operates within the mTOR signaling pathway known for its role in controlling cell growth and proliferation. It interacts with proteins like mTORC1 which is a known regulator of cellular metabolism and protein synthesis. These interactions allow p70 to integrate signals from other kinases like Akt influencing downstream responses including glucose metabolism.

P70 S6 Kinase beta/SRK links to conditions such as cancer and type 2 diabetes. Aberrant activation of P70 through the mTOR pathway contributes significantly to tumorigenesis due to its influence on cell growth and survival. Additionally in type 2 diabetes dysregulated signaling of P70 affects insulin sensitivity often in conjunction with related enzymes such as Akt and mTOR.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Phosphorylates specifically ribosomal protein S6 (PubMed : 29750193). Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7 (PubMed : 29750193).

See full target information RPS6KB2 phospho S371

style

left-column

文献 (20)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 15: PubMed36615799

2022

L-Theanine Regulates the Abundance of Amino Acid Transporters in Mice Duodenum and Jejunum via the mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kehong Liu,Yingqi Peng,Ling Lin,Zhihua Gong,Wenjun Xiao,Yinhua Li

Cell death & disease 13:403 PubMed35468874

2022

SHANK1 facilitates non-small cell lung cancer processes through modulating the ubiquitination of Klotho by interacting with MDM2.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Chen,Hongye Zhao,Min Li,Quan She,Wen Liu,Jiayi Zhang,Weihong Zhao,Shuhong Huang,Jianqing Wu

Scientific reports 11:24005 PubMed34907287

2021

Spontaneous formation of neutrophil extracellular traps is associated with autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Guo,Fei Gao,Xin Wang,Zhenzhen Pan,Qian Wang,Shiyao Xu,Shanshan Pan,Ling Li,Deyu Zhao,Jun Qian

Open medicine (Warsaw, Poland) 16:1364-1371 PubMed34589612

2021

miR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB1.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Juan Zhang,Fang Wang,Pei-Yan Qi,Wei-Yan Zhou,Bing Wang

The Journal of biological chemistry 295:14125-14139 PubMed32605923

2020

Role of long noncoding RNA MEG3/miR-378/GRB2 axis in neuronal autophagy and neurological functional impairment in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Cheng Luo,Ting-Zhuang Yi,Fu-Gao Huang,Ying Wei,Xiao-Peng Luo,Qi-Sheng Luo

Journal of cellular physiology 235:7816-7826 PubMed31907929

2020

α5-nAChR modulates melanoma growth through the Notch1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

NingNing Dang,Xianguang Meng,Guojing Qin,Yunhe An,QianQian Zhang,Xiaoyan Cheng,Shuhong Huang

Molecular medicine reports 21:470-477 PubMed31746379

2019

Mechanical stress promotes biological functions of C2C12 myoblasts by activating PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Da,Yongbin Mou,Mengjia Wang,Xiao Yuan,Fuhua Yan,Weidong Lan,Fang Zhang

Cell cycle (Georgetown, Tex.) 18:2239-2254 PubMed31378124

2019

Silencing of EPCAM suppresses hepatic fibrosis and hepatic stellate cell proliferation in mice with alcoholic hepatitis via the PI3K/Akt/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Zhang,Huiqing Wen,Jun Weng,Lei Feng,Hongya Liu,Xiaojun Hu,Fanhong Zeng

BioMed research international 2019:1528278 PubMed31355248

2019

miR-451 Silencing Inhibited Doxorubicin Exposure-Induced Cardiotoxicity in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Weiguo Wan,Tao Chen,Suiyang Tong,Xuejun Jiang,Wanli Liu

Cancer cell international 19:86 PubMed30996686

2019

Stimulative role of ST6GALNAC1 in proliferation, migration and invasion of ovarian cancer stem cells via the Akt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Yan Wang,Yun-Xia Cao,Xiao Zhou,Bing Wei,Lei Zhan,Shi-Ying Sun

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-P70 S6 Kinase beta/SRK (phospho S371)抗体[EPR2132]