Anti-p63RhoGEF抗体(ab127690)
Key features and details
- Rabbit polyclonal to p63RhoGEF
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-p63RhoGEF抗体 -
描述
兔多克隆抗体to p63RhoGEF -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human p63RhoGEF aa 200-450.
Database link: Q86VW2-1 -
阳性对照
- NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; Human breast carcinoma tissue.
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常规说明
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab127690于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 64 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 64 kDa. |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis. -
组织特异性
Isoform 1 and isoform 2 are highly expressed in excitable tissues, such as brain, heart and muscle. Also detected in kidney and liver. -
序列相似性
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain. -
结构域
The guanine nucleotide exchange activity is autoinhibited by the PH domain. -
细胞定位
Cell membrane. Cytoplasm > myofibril > sarcomere. Highly colocalizes with actin regions. - Information by UniProt
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数据库链接
- Entrez Gene: 115557 Human
- Omim: 610215 Human
- SwissProt: Q86VW2 Human
- Unigene: 61581 Human
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别名
- ARHGEF25 antibody
- ARHGP_HUMAN antibody
- GEFT antibody
see all
图片
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Western blot - Anti-p63RhoGEF antibody (ab127690)Anti-p63RhoGEF antibody (ab127690) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 64 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63RhoGEF antibody (ab127690)ab127690, at a dilution of 1/250, staining p63RhoGEF in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab127690 被引用在 4 文献中.
- Wang Y et al. MicroRNA-29 family inhibits rhabdomyosarcoma formation and progression by regulating GEFT function. Am J Transl Res 12:1136-1154 (2020). PubMed: 32269740
- Wang Y et al. Long Non-Coding RNA LINC00355 Promotes the Development and Progression of Colorectal Cancer by Elevating Guanine Nucleotide Exchange Factor T Expression via RNA Binding Protein lin-28 Homolog A. Front Oncol 10:582669 (2020). PubMed: 33381451
- Wang Y et al. GEFT protein expression in digestive tract malignant tumors and its clinical significance. Oncol Lett 18:5577-5590 (2019). PubMed: 31620201
- Liu C et al. Epigenetically upregulated GEFT-derived invasion and metastasis of rhabdomyosarcoma via epithelial mesenchymal transition promoted by the Rac1/Cdc42-PAK signalling pathway. EBioMedicine 50:122-134 (2019). PubMed: 31761617