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AB53039

Anti-p63 抗体

Anti-p63 antibody

5

(10 Reviews)

|

(65 Publications)

Rabbit Polyclonal P63 antibody. Suitable for WB, ICC/IF and reacts with African green monkey, Human samples. Cited in 65 publications. Immunogen corresponding to Synthetic Peptide within Human TP63.

查看别名

KET, P63, P73H, P73L, TP73L, TP63, Tumor protein 63, p63, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor KET, Transformation-related protein 63, Tumor protein p73-like, p40, p51, CUSP, p73L

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody (AB53039)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody (AB53039)

Immunofluorescence analysis of A549 (Human lung carcinoma cell line) cells using ab53039 at 1/100 dilution. The picture on the right is blocked with the synthesized peptide.

Western blot - Anti-p63 antibody (AB53039)
  • WB

Unknown

Western blot - Anti-p63 antibody (AB53039)

All lanes:

Western blot - Anti-p63 antibody (ab53039) at 1/500 dilution

Lane 1:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract

Lane 2:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract with peptide

Predicted band size: 77 kDa

Observed band size: 77 kDa

false

Western blot - Anti-p63 antibody (AB53039)
  • WB

Unknown

Western blot - Anti-p63 antibody (AB53039)

All lanes:

Western blot - Anti-p63 antibody (ab53039) at 1/1000 dilution

Lane 1:

Human p63 full length protein (<a href='/products/unavailable/human-p63-full-length-protein-ab101717'>ab101717</a>) at 0.1 µg

Lane 2:

Human p63 full length protein (<a href='/products/unavailable/human-p63-full-length-protein-ab101717'>ab101717</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 77 kDa

true

Exposure time: 10s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human, African green monkey

应用

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human TP63. The exact immunogen used to generate this antibody is proprietary information.

Q9H3D4

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替代产品

Primary Antibodies

AB238079

Anti-p63 antibody [TP63/2427]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [TP63/2427] (AB238079)

  • 0
  • 0
Primary Antibodies

AB92581

Anti-p63 (phospho S395) antibody

Western blot - Anti-p63 (phospho S395) antibody (AB92581)

  • 0
  • 0
Primary Antibodies

AB110038

Anti-p63 antibody [4E5]

Flow Cytometry - Anti-p63 antibody [4E5] (AB110038)

  • 4
  • 4
Primary Antibodies

AB735

Anti-p63 antibody [4A4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [4A4] (AB735)

  • 5
  • 12
Primary Antibodies

AB216493

Anti-p63 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody (AB216493)

  • 0
  • 0
Primary Antibodies

AB169707

Anti-p63 antibody [I27-I]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [I27-I] (AB169707)

  • 0
  • 0
Primary Antibodies

AB179874

Anti-p63 antibody [C24-I] - N-terminal

  • 0
  • 0
Primary Antibodies

AB246728

Alexa Fluor® 647 Anti-p63 antibody [EPR5701]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-p63 antibody [EPR5701] (AB246728)

  • 0
  • 0
Primary Antibodies

AB202861

Biotin Anti-p63 antibody [EPR5701]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-p63 antibody [EPR5701] (AB202861)

  • 0
  • 0
Primary Antibodies

AB202357

HRP Anti-p63 antibody [EPR5701]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-p63 antibody [EPR5701] (AB202357)

  • 0
  • 0
Primary Antibodies

AB124762

Anti-p63 antibody [EPR5701]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] (AB124762)

  • 4
  • 13
Primary Antibodies

AB32353

Anti-p63 antibody [Y289]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody [Y289] (AB32353)

  • 5
  • 1
Primary Antibodies

AB214790

Anti-p63 antibody [EPR5701] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-p63 antibody [EPR5701] - BSA and Azide free (AB214790)

  • 0
  • 0
Primary Antibodies

AB97865

Anti-p63 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody (AB97865)

  • 5
  • 1

搭配使用产品

Assay Kits

AB234564

Human P63 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human P63 ELISA Kit (AB234564)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB52635

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

  • 5
  • 11
Primary Antibodies

AB119695

Anti-Cytokeratin 14 antibody [SP53]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [SP53] (AB119695)

  • 5
  • 6
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The p63 protein also known as TP63 or KET is a transcription factor that plays a role in the regulation of cell cycle and apoptosis. With a molecular mass of approximately 77 kDa it expresses predominantly in epithelial tissues such as those in the skin breast and prostate. The expression pattern of p63 makes it a useful marker in developmental biology and cancer diagnosis. Antibodies like '4A4' specifically recognize p63 in various assays including immunohistochemistry.
Biological function summary

P63 participates in the control of epithelial cell differentiation and proliferation. It functions as a member of the p53 family which includes p53 and p73. This family forms an important part of the complex regulatory network that maintains genomic stability. Research using methods like p63 staining in immunohistochemistry highlights its critical role in stratified epithelia. The gene encodes several isoforms with different biological activities impacting processes such as cellular senescence and development.

Pathways

P63 integrates into various signaling pathways that influence cellular growth and adherence. It notably impacts the Notch and Wnt pathways both of which are critical for developmental processes and cancer progression. Through these pathways p63 interacts with proteins like β-catenin and Notch receptors facilitating cross-talk that influences cell fate decisions. Its function relates closely to other transcription factors forming feedback loops that reinforce its regulatory impact.

P63 shows strong associations with ectodermal dysplasia and different types of cancers like squamous cell carcinoma. Mutations and altered expressions of p63 contribute to these pathologies by disrupting normal cell proliferation and differentiation. The protein interacts closely with p73 in cancer suggesting a compensatory role when p63 function is compromised. Understanding these connections can guide the development of targeted therapies and improve diagnostic accuracy.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.
See full target information TP63
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文献 (65)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed39545409

2024

MGA loss-of-function variants cause premature ovarian insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyan Tang,Ting Guo,Chengcheng Song,Lingbo Wang,Jun Zhang,Aleksandar Rajkovic,Xiaoqi Lin,Shiling Chen,Yujun Liu,Weidong Tian,Bangguo Wu,Shixuan Wang,Wenwen Wang,Yunhui Lai,Ao Wang,Shuhua Xu,Li Jin,Hanni Ke,Shidou Zhao,Yan Li,Yingying Qin,Feng Zhang,Zi-Jiang Chen

iScience 26:107568 PubMed37622003

2023

Estrogen receptor alpha regulates uterine epithelial lineage specification and homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jason A Rizo,Kimberly M Davenport,Wipawee Winuthayanon,Thomas E Spencer,Andrew M Kelleher

The Journal of investigative dermatology 144:73-83.e10 PubMed37543242

2023

ΔNp63 Regulates Homeostasis, Stemness, and Suppression of Inflammation in the Adult Epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher E Eyermann,Xi Chen,Ozge S Somuncu,Jinyu Li,Alexander N Joukov,Jiang Chen,Evguenia M Alexandrova

mBio 13:e0148522 PubMed35862773

2022

A Retinol Derivative Inhibits SARS-CoV-2 Infection by Interrupting Spike-Mediated Cellular Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Liangqin Tong,Lin Wang,Shumin Liao,Xiaoping Xiao,Jing Qu,Chunli Wu,Yibin Zhu,Wanbo Tai,Yanhong Huang,Penghua Wang,Liang Li,Renli Zhang,Ye Xiang,Gong Cheng

International journal of oral science 14:26 PubMed35581183

2022

METTL3-mediated mA RNA methylation regulates dorsal lingual epithelium homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuchan Xiong,Caojie Liu,Xin Zheng,Xinyi Zhou,Kexin Lei,Xiaohan Zhang,Qian Wang,Weimin Lin,Ruizhan Tong,Ruoshi Xu,Quan Yuan

Nature protocols 17:1658-1690 PubMed35546639

2022

Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection.

Applications

Unspecified application

Species

Unspecified reactive species

Rajendra Kumar Gurumurthy,Stefanie Koster,Naveen Kumar,Thomas F Meyer,Cindrilla Chumduri

Nature metabolism 4:547-558 PubMed35534727

2022

A glucose-like metabolite deficient in diabetes inhibits cellular entry of SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Liangqin Tong,Xiaoping Xiao,Min Li,Shisong Fang,Enhao Ma,Xi Yu,Yibin Zhu,Chunli Wu,Deyu Tian,Fan Yang,Jing Sun,Jing Qu,Nianzhen Zheng,Shumin Liao,Wanbo Tai,Shengyong Feng,Liming Zhang,Yuhan Li,Lin Wang,Xuelian Han,Shihui Sun,Long Yang,Hui Zhong,Jincun Zhao,Wenjun Liu,Xiaohui Liu,Penghua Wang,Liang Li,Guangyu Zhao,Renli Zhang,Gong Cheng

Cancers 14: PubMed35565305

2022

EMT, Stemness, and Drug Resistance in Biological Context: A 3D Tumor Tissue/In Silico Platform for Analysis of Combinatorial Treatment in NSCLC with Aggressive KRAS-Biomarker Signatures.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Peindl,Claudia Göttlich,Samantha Crouch,Niklas Hoff,Tamara Lüttgens,Franziska Schmitt,Jesús Guillermo Nieves Pereira,Celina May,Anna Schliermann,Corinna Kronenthaler,Danjouma Cheufou,Simone Reu-Hofer,Andreas Rosenwald,Elena Weigl,Thorsten Walles,Julia Schüler,Thomas Dandekar,Sarah Nietzer,Gudrun Dandekar

Frontiers in oncology 12:818437 PubMed35530312

2022

Intrinsic Differences in Spatiotemporal Organization and Stromal Cell Interactions Between Isogenic Lung Cancer Cells of Epithelial and Mesenchymal Phenotypes Revealed by High-Dimensional Single-Cell Analysis of Heterotypic 3D Spheroid Models.

Applications

Unspecified application

Species

Unspecified reactive species

Maria L Lotsberg,Gro V Røsland,Austin J Rayford,Sissel E Dyrstad,Camilla T Ekanger,Ning Lu,Kirstine Frantz,Linda E B Stuhr,Henrik J Ditzel,Jean Paul Thiery,Lars A Akslen,James B Lorens,Agnete S T Engelsen

The European respiratory journal 59: PubMed34385275

2022

expression identifies airway basal cells with high proliferative capacity and restricts lung squamous cell carcinoma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Succony,Sandra Gómez-López,Adam Pennycuick,Ahmed S N Alhendi,Derek Davies,Sarah E Clarke,Kate H C Gowers,Nicholas A Wright,Kim B Jensen,Sam M Janes
View all publications
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