重组Anti-p60 CAF1/MPP7抗体[EPR6105] (ab109442)
![Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)](https://www.abcam.cn/ps/products/109/ab109442/Images/ab109442-122293-anti-p60-caf1-antibody-epr6105-western-blot.jpg "Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6105] to p60 CAF1/MPP7
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-p60 CAF1/MPP7抗体[EPR6105]
参阅全部 p60 CAF1/MPP7 一抗 -
描述
兔单克隆抗体[EPR6105] to p60 CAF1/MPP7 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
不与反应: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat, HeLa, 293T, and Caco2 cell lysates; IHC: Human colon and tonsil tissues. Flow Cyt: HeLa cells. IP: HeLa.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6105 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109442于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
|
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| IP |
1/10 - 1/100.
|
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 61 kDa. |
|
IP
1/10 - 1/100. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
靶标
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功能
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. -
序列相似性
Belongs to the WD repeat HIR1 family.
Contains 7 WD repeats. -
发展阶段
Active complex is found in G1, S and G2 phases. -
翻译后修饰
Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases. -
细胞定位
Nucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase. - Information by UniProt
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数据库链接
- Entrez Gene: 8208 Human
- Omim: 601245 Human
- SwissProt: Q13112 Human
- Unigene: 75238 Human
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别名
- CAF 1 subunit B antibody
- CAF 1A antibody
- CAF I 60 kDa subunit antibody
see all
图片
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Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)All lanes : Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)](https://www.abcam.cn/ps/products/109/ab109442/Images/ab109442-201921-anti-p60-caf1-antibody-epr6105-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)Overlay histogram showing HeLa cells stained with ab109442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109442, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)](https://www.abcam.cn/ps/products/109/ab109442/Images/ab109442-122292-anti-p60-caf1-antibody-epr6105-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human colon tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)](https://www.abcam.cn/ps/products/109/ab109442/Images/ab109442-122291-anti-p60-caf1-antibody-epr6105-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human tonsil tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)](https://www.abcam.cn/ps/products/109/ab109442/Images/ab109442-6-anti-p60-caf1-mpp7-antibody-epr6105-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)p60 CAF1/MPP7
was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with 109442 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab109442 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109442 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)](https://www.abcam.cn/ps/products/109/ab109442/Images/ab109442-5-benefits-of-recombinant-antibodies.png)
Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab109442 被引用在 7 文献中.
- Shrestha RL et al. The histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stability. J Cell Sci 136:N/A (2023). PubMed: 37129573
- Geis FK et al. CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in infection. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35074917
- Tachiwana H et al. Chromatin structure-dependent histone incorporation revealed by a genome-wide deposition assay. Elife 10:N/A (2021). PubMed: 33970102
- Shen Y et al. Multi-Omics Integrative Analysis Uncovers Molecular Subtypes and mRNAs as Therapeutic Targets for Liver Cancer. Front Med (Lausanne) 8:654635 (2021). PubMed: 34109194
- Yang S et al. CAF-1/p150 promotes cell proliferation, migration, invasion and predicts a poor prognosis in patients with cervical cancer. Oncol Lett 20:2338-2346 (2020). PubMed: 32782551
- Duan Y et al. CHAF1B promotes proliferation and reduces apoptosis in 95-D lung cancer cells and predicts a poor prognosis in non-small cell lung cancer. Oncol Rep 41:2518-2528 (2019). PubMed: 30720130
- Piquet S et al. The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage. Mol Cell 72:888-901.e7 (2018). PubMed: 30344095