Anti-P5CS抗体(ab223713)
Key features and details
- Rabbit polyclonal to P5CS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-P5CS抗体
参阅全部 P5CS 一抗 -
描述
兔多克隆抗体to P5CS -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Orangutan
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免疫原
Recombinant fragment corresponding to Human P5CS aa 50-250.
Database link: P54886 -
阳性对照
- WB: NIH/3T3, NBT-II and U-251 cell lysates. IHC-P: Human colon, testis and cerebral cortex tissue. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223713于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 87 kDa.
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| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 87 kDa. |
|
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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通路
Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.
Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. -
疾病相关
Defects in ALDH18A1 are the cause of mental retardation-joint hypermobility-skin laxity with or without metabolic abnormalities (MRJHSL) [MIM:612652]. Clinical manifestations include microcephaly, progressive neurologic dysfunction, mental retardation, progeroid appearance, joint hypermobility, skin laxity and hyperelasticity, cataracts. Some patients manifest metabolic disturbances such as hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia. -
序列相似性
In the N-terminal section; belongs to the glutamate 5-kinase family.
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5832 Human
- Entrez Gene: 56454 Mouse
- Entrez Gene: 100173488 Orangutan
- Entrez Gene: 361755 Rat
- Omim: 138250 Human
- SwissProt: P54886 Human
- SwissProt: Q9Z110 Mouse
- SwissProt: Q5R4M8 Orangutan
see all -
形式
P5CS catalyzes the ATP- and NADPH-dependent conversion of L-glutamate to glutamic gamma-semialdehyde, which is the metabolic precursor for proline biosynthesis. There are 2 isoforms produced by alternative splicing. -
别名
- 2810433K04Rik antibody
- AI429789 antibody
- Aldehyde dehydrogenase 18 family member A1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (ab223713)
Paraffin embedded sections of human testis were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (ab223713)Paraffin embedded sections of human colon were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (ab223713)Paraffin embedded sections of human cerebral cortex were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.
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Western blot - Anti-P5CS antibody (ab223713)All lanes : Anti-P5CS antibody (ab223713) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 87 kDa -
Western blot - Anti-P5CS antibody (ab223713)All lanes : Anti-P5CS antibody (ab223713) at 1/20 dilution
Lane 1 : Caco-2 cell lysate transfected with control siRNA
Lane 2 : Caco-2 cell lysate transfected with target specific siRNA probe #1
Lane 3 : Caco-2 cell lysate transfected with target specific siRNA probe #2
Predicted band size: 87 kDa -
Western blot - Anti-P5CS antibody (ab223713)All lanes : Anti-P5CS antibody (ab223713) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat bladder tumor cell line) cell lysate
Predicted band size: 87 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-P5CS antibody (ab223713)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for P5CS (green) using ab223713 at 4 µg/ml in ICC/IF.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab223713 尚未被引用在任何文献中。