Anti-p53R2抗体(ab8105)

Reviews (2)Q&A (1)References (27)
Western blot - Anti-p53R2 antibody (ab8105)

    Key features and details

    • Rabbit polyclonal to p53R2
    • Suitable for: WB, ICC, ICC/IF, IHC-P
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-p53R2抗体
      参阅全部 p53R2 一抗

    • 描述

      兔多克隆抗体to p53R2

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WB, ICC, ICC/IF, IHC-Pmore details

    • 种属反应性

      与反应: Mouse, Rat, Human

    • 免疫原

      Synthetic peptide:

      GDPERPEAAGLDQDER

      , corresponding to amino acids 2-17 of Human p53R2.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab8105于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).
    ICC
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration. PubMed: 18997010
    IHC-P
    Use at an assay dependent concentration.
    说明
    WB
    Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).
    ICC
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration. PubMed: 18997010
    IHC-P
    Use at an assay dependent concentration.

    靶标

    • 功能

      Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.

    • 组织特异性

      Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma.

    • 通路

      Genetic information processing; DNA replication.

    • 疾病相关

      Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A) [MIM:612075]. A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy.
      Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B) [MIM:612075]. A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy.
      Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5) [MIM:613077]. A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.

    • 序列相似性

      Belongs to the ribonucleoside diphosphate reductase small chain family.

    • 细胞定位

      Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage.
    • Information by UniProt

    • 数据库链接

      • 别名

        • DKFZp686M05248 antibody
        • MGC102856 antibody
        • MGC42116 antibody
        • MTDPS8A antibody
        • MTDPS8B antibody
        • p53 inducible ribonucleotide reductase small subunit 2 homolog antibody
        • p53 inducible ribonucleotide reductase small subunit 2 like protein antibody
        • P53 inducible ribonucleotide reductase small subunit 2 short form beta antibody
        • p53 R2 antibody
        • p53-inducible ribonucleotide reductase small subunit 2-like protein antibody
        • p53R2 antibody
        • Ribonucleoside diphosphate reductase M2 subunit B antibody
        • Ribonucleoside-diphosphate reductase subunit M2 B antibody
        • Ribonucleotide reductase M2 B (TP53 inducible) antibody
        • Ribonucleotide reductase M2 B antibody
        • Ribonucleotide reductase small subunit like 2 p53 inducible antibody
        • RIR2B_HUMAN antibody
        • RRM 2B antibody
        • RRM2B antibody
        • TP53 inducible ribonucleotide reductase M2 B antibody
        • TP53-inducible ribonucleotide reductase M2 B antibody
        see all

      图片

      • Western blot - Anti-p53R2 antibody (ab8105)
        Western blot - Anti-p53R2 antibody (ab8105)
        All lanes : Anti-p53R2 antibody (ab8105) at 1 µg/ml

        Lane 1 : 3T3 cell lysate with absence of blocking peptide
        Lane 2 : 3T3 cell lysate with presence of blocking peptide

        Predicted band size: 41 kDa

      文献 (27)

      发表研究结果有使用 ab8105？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab8105 被引用在 27 文献中.

      • Rudd SG  et al. Ribonucleotide reductase inhibitors suppress SAMHD1 ara-CTPase activity enhancing cytarabine efficacy. EMBO Mol Med 12:e10419 (2020). PubMed: 31950591
      • Zhang Q  et al. miR-942 promotes proliferation and metastasis of hepatocellular carcinoma cells by inhibiting RRM2B. Onco Targets Ther 12:8367-8378 (2019). PubMed: 31632084
      • Ding Y  et al. Integrative Analysis Reveals Across-Cancer Expression Patterns and Clinical Relevance of Ribonucleotide Reductase in Human Cancers. Front Oncol 9:956 (2019). PubMed: 31637211
      • Chen J  et al. CD8+ tumor-infiltrating lymphocytes as a novel prognostic biomarker in lung sarcomatoid carcinoma, a rare subtype of lung cancer. Cancer Manag Res 10:3505-3511 (2018). PubMed: 30271199
      • Fu Y  et al. Nuclear RNR-a antagonizes cell proliferation by directly inhibiting ZRANB3. Nat Chem Biol 14:943-954 (2018). PubMed: 30150681
      View all Publications for this product

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Immunoprecipitation abreview for Anti-p53R2 antibody

       Good
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (GBM6 cells)
      Total protein in input
      200 µg
      Treatment
      50 uM DMSO for 24 and 72 hours, 50 uM TMZ for 24 and 72 hours
      Immuno-precipitation step
      Protein A/G
      Specification
      GBM6 cells
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Feb 05 2021

      Western blot abreview for Anti-p53R2 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (U2OS)
      Specification
      U2OS
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Sep 01 2005

      Question

      Dear All,



      Please let us know about the following that many slides should have been available for the concentrated antibodies after dilution and how many slides can be prepeared with the prediluted slides. Also let us know the about the deteation kit and DAB ab64264 and



      ab3223

      ab63340

      ab74179

      ab8105

      ab27595 (pre-diluted)

      ab27596 (pre-diluted)

      ab15467 (pre-diluted)


      ab64264 -sufficient for how many slides?

      Awaiting your early response.

      Sincere regards,

      Read More
      Answer

      Thank you for your inquiry.

      Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted antibodies are used neat there might be reasons to use 50ul per slide or maybe even 100ul per slide.

      These conditions include: size of the sample, tissue type, lab experience, diluent buffer, species, antigen retrieval, incubation time, etc.

      For the products you mentioned I can give a estimate for the pre-diluted antibodies of ca 140- 280slides, for the other antibodies 500 - 100 slides and for the detection kit 150 - 300 slides.

      I hope this information is helpful.

      Read More

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