Anti-p53R2抗体(ab8105)
Key features and details
- Rabbit polyclonal to p53R2
- Suitable for: WB, ICC, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-p53R2抗体
参阅全部 p53R2 一抗 -
描述
兔多克隆抗体to p53R2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide:
GDPERPEAAGLDQDER
, corresponding to amino acids 2-17 of Human p53R2. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).
|
| ICC |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 18997010
|
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| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa). |
|
ICC
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 18997010 |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. -
组织特异性
Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma. -
通路
Genetic information processing; DNA replication. -
疾病相关
Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A) [MIM:612075]. A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy.
Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B) [MIM:612075]. A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy.
Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5) [MIM:613077]. A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism. -
序列相似性
Belongs to the ribonucleoside diphosphate reductase small chain family. -
细胞定位
Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage. - Information by UniProt
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数据库链接
- Entrez Gene: 50484 Human
- Entrez Gene: 382985 Mouse
- Entrez Gene: 299976 Rat
- Omim: 604712 Human
- SwissProt: Q7LG56 Human
- SwissProt: Q6PEE3 Mouse
- Unigene: 512592 Human
- Unigene: 24738 Mouse
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别名
- DKFZp686M05248 antibody
- MGC102856 antibody
- MGC42116 antibody
see all
图片
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Western blot - Anti-p53R2 antibody (ab8105)All lanes : Anti-p53R2 antibody (ab8105) at 1 µg/ml
Lane 1 : 3T3 cell lysate with absence of blocking peptide
Lane 2 : 3T3 cell lysate with presence of blocking peptide
Predicted band size: 41 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab8105 被引用在 27 文献中.
- Rudd SG et al. Ribonucleotide reductase inhibitors suppress SAMHD1 ara-CTPase activity enhancing cytarabine efficacy. EMBO Mol Med 12:e10419 (2020). PubMed: 31950591
- Zhang Q et al. miR-942 promotes proliferation and metastasis of hepatocellular carcinoma cells by inhibiting RRM2B. Onco Targets Ther 12:8367-8378 (2019). PubMed: 31632084
- Ding Y et al. Integrative Analysis Reveals Across-Cancer Expression Patterns and Clinical Relevance of Ribonucleotide Reductase in Human Cancers. Front Oncol 9:956 (2019). PubMed: 31637211
- Chen J et al. CD8+ tumor-infiltrating lymphocytes as a novel prognostic biomarker in lung sarcomatoid carcinoma, a rare subtype of lung cancer. Cancer Manag Res 10:3505-3511 (2018). PubMed: 30271199
- Fu Y et al. Nuclear RNR-a antagonizes cell proliferation by directly inhibiting ZRANB3. Nat Chem Biol 14:943-954 (2018). PubMed: 30150681