Anti-p50 dynamitin抗体(ab124285)
Key features and details
- Rabbit polyclonal to p50 dynamitin
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-p50 dynamitin抗体
参阅全部 p50 dynamitin 一抗 -
描述
兔多克隆抗体to p50 dynamitin -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey, Xenopus tropicalis, Elephant
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免疫原
Synthetic peptide corresponding to Human p50 dynamitin aa 1-50 (N terminal).
Database link: NP_006391.1 -
阳性对照
- Jurkat, 293T, HeLa and NIH3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab124285 was affinity purified using an epitope specific to p50 dynamitin immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124285于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000.
|
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| IP |
Use at 2-10 µg/mg of lysate.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. |
|
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. -
序列相似性
Belongs to the dynactin subunit 2 family. -
细胞定位
Cytoplasm > cytoskeleton > centrosome. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10540 Human
- Entrez Gene: 69654 Mouse
- Entrez Gene: 299850 Rat
- Omim: 607376 Human
- SwissProt: Q3ZCF0 Cow
- SwissProt: Q13561 Human
- SwissProt: Q99KJ8 Mouse
- SwissProt: Q6AYH5 Rat
see all -
别名
- 50 kD dynein associated polypeptide antibody
- 50 kDa dynein associated polypeptide antibody
- 50 kDa dynein-associated polypeptide antibody
see all
图片
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Western blot - Anti-p50 dynamitin antibody (ab124285)All lanes : Anti-p50 dynamitin antibody (ab124285) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds -
Immunoprecipitation - Anti-p50 dynamitin antibody (ab124285)ab124285 at 1 µg/ml staining p50 dynamitin in Jurkat whole cell lysate immunoprecipitated using ab124285 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 44 kDa. Exposure time: 3 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab124285 尚未被引用在任何文献中。