Anti-P4HA2抗体(ab70887)
Key features and details
- Rabbit polyclonal to P4HA2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-P4HA2抗体
参阅全部 P4HA2 一抗 -
描述
兔多克隆抗体to P4HA2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide corresponding to a region between residue 250 and 300 of human P4HA2. (NP_004190.1)
-
阳性对照
- HeLa and 293T whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70887于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
|
| IP |
Use at 2-5 µg/mg of lysate.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
|
IP
Use at 2-5 µg/mg of lysate. |
靶标
-
功能
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. -
序列相似性
Belongs to the P4HA family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 TPR repeat. -
细胞定位
Endoplasmic reticulum lumen. - Information by UniProt
-
数据库链接
- Entrez Gene: 8974 Human
- Omim: 600608 Human
- SwissProt: O15460 Human
- Unigene: 519568 Human
-
别名
- 2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody
- 4 PH alpha 2 antibody
- 4-PH alpha-2 antibody
see all
图片
-
Western blot - Anti-P4HA2 antibody (ab70887)All lanes : Anti-P4HA2 antibody (ab70887) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Additional bands at: 28 kDa, 33 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunoprecipitation - Anti-P4HA2 antibody (ab70887)HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70887 at 1 µg/ml for WB subsequent to IP at 3 µg/mg lysate.
Lane 2: control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds. -
Western blot - Anti-P4HA2 antibody (ab70887)Image courtesy of an anonymous Abreview.All lanes : Anti-P4HA2 antibody (ab70887) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC12 cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Additional bands at: 35 kDa (possible degradation product)
Exposure time: 5 minutes
Good immunoreactivity observed in RIE-1 cell lysate that is consistent with the mobility of full-length P4HA2. A faster migrating species of ~35 kDa has been commonly observed with other antibodies (including this one) that could be a degradation product or alternatively spliced form of P4HA2. Further charcaterisation is required.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab70887 被引用在 1 文献中.
- Pankova D et al. RASSF1A controls tissue stiffness and cancer stem-like cells in lung adenocarcinoma. EMBO J 38:e100532 (2019). PubMed: 31268606