Anti-P4HA1抗体(ab59497)
Key features and details
- Goat polyclonal to P4HA1
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-P4HA1抗体
参阅全部 P4HA1 一抗 -
描述
山羊多克隆抗体to P4HA1 -
宿主
Goat -
特异性
This antibody is expected to recognise both reported isoforms. -
经测试应用
适用于: ICCmore details -
种属反应性
与反应: Human
预测可用于: Chicken, Cow, Dog, Pig, Zebrafish, Orangutan
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免疫原
Synthetic peptide within Human P4HA1 aa 400-500 (internal sequence) (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P13674 -
阳性对照
- ICC: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59497于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
ICC
Use a concentration of 10 µg/ml. |
靶标
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功能
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. -
序列相似性
Belongs to the P4HA family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 TPR repeat. -
细胞定位
Endoplasmic reticulum lumen. - Information by UniProt
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数据库链接
- Entrez Gene: 5033 Human
- SwissProt: P13674 Human
- Unigene: 500047 Human
- Unigene: 593005 Human
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别名
- 2-oxoglutarate-4-dioxygenase subunit alpha-1 antibody
- 4-hydroxylase, alpha I subunit antibody
- 4-PH alpha-1 antibody
see all
图片
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Immunocytochemistry - Anti-P4HA1 antibody (ab59497)
Immunocytochemistry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells labeling P4HA1 with ab59497 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab59497 被引用在 8 文献中.
- Zhu H et al. The role of vesicle trafficking genes in osteoblast differentiation and function. Sci Rep 13:16079 (2023). PubMed: 37752218
- Xu X et al. Silencing of NONO inhibits abdominal aortic aneurysm in apolipoprotein E-knockout mice via collagen deposition and inflammatory inhibition. J Cell Mol Med 23:7449-7461 (2019). PubMed: 31512366
- Zhao T et al. Liraglutide alleviates cardiac fibrosis through inhibiting P4ha-1 expression in STZ-induced diabetic cardiomyopathy. Acta Biochim Biophys Sin (Shanghai) 51:293-300 (2019). PubMed: 30883649
- Feng Q et al. Integrative proteomics and immunochemistry analysis of the factors in the necrosis and repair in acetaminophen-induced acute liver injury in mice. J Cell Physiol 234:6561-6581 (2019). PubMed: 30417486
- Zhao T et al. Liraglutide protects high-glucose-stimulated fibroblasts by activating the CD36-JNK-AP1 pathway to downregulate P4HA1. Biomed Pharmacother 118:109224 (2019). PubMed: 31349139
- Corporan D et al. Temporal changes in myocardial collagen, matrix metalloproteinases, and their tissue inhibitors in the left ventricular myocardium in experimental chronic mitral regurgitation in rodents. Am J Physiol Heart Circ Physiol 315:H1269-H1278 (2018). PubMed: 30141979
- Wang XL et al. Rosuvastatin Attenuates CD40L-Induced Downregulation of Extracellular Matrix Production in Human Aortic Smooth Muscle Cells via TRAF6-JNK-NF-?B Pathway. PLoS One 11:e0153919 (2016). WB ; Human . PubMed: 27120457
- Wong L et al. Prostatic inflammation induces fibrosis in a mouse model of chronic bacterial infection. PLoS One 9:e100770 (2014). IHC-P ; Mouse . PubMed: 24950301