AB231639

Anti-p40-phox抗体

Name: p40-phox抗体 (ab231639)| Abcam中文官网
Brand: Abcam Limited
SKU: AB231639
Price: 4748 CNY
Availability: InStock

Anti-p40-phox antibody

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(1 Publication)

Rabbit Polyclonal p40-phox antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Neutrophil cytosol factor 4 aa 100 to C-terminus.

查看别名

Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, p40-phox, p40phox, Ncf4

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Paraffin-embedded mouse liver tissue stained for p40-phox using ab231639 at 20 μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Paraffin-embedded mouse kidney tissue stained for p40-phox using ab231639 at 20 μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Paraffin-embedded mouse spleen tissue stained for p40-phox using ab231639 at 20 μg/ml in immunohistochemical analysis.

Supplier Data

Western blot - Anti-p40-phox antibody (AB231639)

All lanes:

Western blot - Anti-p40-phox antibody (ab231639) at 3 µg/mL

All lanes:

Mouse spleen lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-p40-phox antibody (AB231639)

All lanes:

Western blot - Anti-p40-phox antibody (ab231639) at 3 µg/mL

All lanes:

Recombinant mouse p40-phox protein.

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 39 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Mouse Neutrophil cytosol factor 4 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P97369

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab231639 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

P40-phox also known as neutrophil cytosolic factor 4 (NCF4) is a regulatory component of the NADPH oxidase complex. It is expressed in phagocytic cells such as neutrophils and macrophages. The protein plays a role in the production of reactive oxygen species (ROS) during the respiratory burst. The molecular mass of p40-phox is approximately 40 kDa. It interacts with other subunits of the NADPH oxidase complex such as p67-phox and p47-phox contributing to the assembly and activation of the complex.

Biological function summary

P40-phox participates in innate immune responses by regulating the NADPH oxidase complex's function. It acts as a scaffold to facilitate the interaction between cytosolic and membrane components. p40-phox integrates into the cytosolic fraction with other subunits and translocates to the membrane upon stimulation. This translocation is essential for generating superoxide radicals that are involved in defense against pathogens. Its functional domains enable it to bind phosphatidylinositol (34) bisphosphate which anchors the protein to the membrane. p40-phox itself is not an enzyme but modulates the activity of the complex.

Pathways

The p40 protein serves as an important player in the signaling cascade of the respiratory burst. It interacts with the phosphoinositide 3-kinase (PI3K) pathway and cooperates with other proteins like p67-phox and p47-phox through phosphorylation-dependent mechanisms. The p40-phox is phox compatible meaning it can integrate into various signaling modules. Within the oxidative burst pathway it enhances the cytosolic complex's attachment to the membrane affecting the production of ROS.

Mutations in the p40-phox have been connected to chronic granulomatous disease (CGD) characterized by recurrent infections and granuloma formation. The lack of functional p40-phox disrupts the assembly of the NADPH oxidase complex leading to decreased ROS production. This deficiency impairs the ability of phagocytes to eliminate pathogens effectively. p40-phox is also related to inflammatory disorders like inflammatory bowel disease (IBD) as variations in the NCF4 gene affect the immune response. The dysfunction in ROS production impacts the signaling involving p67-phox and p47-phox further influencing disease pathogenesis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.

See full target information Neutrophil cytosol factor 4

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 10: PubMed35625704

2022

Arsenic Activates the ER Stress-Associated Unfolded Protein Response via the Activating Transcription Factor 6 in Human Bronchial Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Wadgaonkar,Zhuoyue Bi,Junmei Wan,Yao Fu,Qian Zhang,Bandar Almutairy,Wenxuan Zhang,Yiran Qiu,Chitra Thakur,Maik Hüttemann,Fei Chen

View all publications

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Abcam Product Promise

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Anti-p40-phox抗体

Anti-p40-phox antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Western blot - Anti-p40-phox antibody (AB231639)

Western blot - Anti-p40-phox antibody (AB231639)