重组Anti-p40 - DeltaNp63抗体[rTP40/3690] - BSA and Azide free (ab273142)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (ab273142)](https://www.abcam.cn/ps/products/273/ab273142/Images/ab273142-391399-Antip40--DeltaNp63-antibody-rTP40-3690-immunohistochemistry-skin-human.JPG "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (ab273142)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [rTP40/3690] to p40 - DeltaNp63 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-p40 - DeltaNp63抗体[rTP40/3690] - BSA and Azide free
参阅全部 p40 - DeltaNp63 一抗 -
描述
小鼠单克隆抗体[rTP40/3690] to p40 - DeltaNp63 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human p40 - DeltaNp63. The exact sequence is proprietary.
Database link: Q9H3D4 -
阳性对照
- IHC-P: Human skin and kidney tissue. Flow Cyt: HeLa cells.
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常规说明
ab273142 is the carrier-free version of ab273135.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
rTP40/3690 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273142于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| Flow Cyt |
Use 1-2µg for 106 cells.
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt
Use 1-2µg for 106 cells. |
靶标
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相关性
Differentiating between lung adenocarcinoma and squamous cell carcinoma is an important distinction with therapeutic implications. Although the most frequently recommended squamous marker p63 is extremely sensitive, it suffers from low specificity due to its reactivity in a substantial proportion of lung adenocarcinomas and other tumor types, particularly lymphomas. p40 is a relatively unknown marker that recognizes ?Np63-a p63 isoform suggested to be highly specific for squamous/basal cells. p40 is equivalent to p63 in sensitivity for squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. These findings strongly support the routine use of p40 in place of p63 for the diagnosis of pulmonary squamous cell carcinoma. -
数据库链接
- SwissProt: Q9H3D4 Human
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别名
- 40 kDa Rab9 effector protein antibody
- 8430412M01Rik antibody
- 9530020d24rik antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (ab273142)
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![Flow Cytometry - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (ab273142)](https://www.abcam.cn/ps/products/273/ab273142/Images/ab273142-391401-Antip40--DeltaNp63-antibody-rTP40-3690-flow-cytometry-HeLa-cells.JPG)
Flow Cytometry - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (ab273142)Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells using ab273135 followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273135).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (ab273142)](https://www.abcam.cn/ps/products/273/ab273142/Images/ab273142-391400-Antip40--DeltaNp63-antibody-rTP40-3690-immunohistochemistry-kidney-human.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (ab273142) -
![Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (ab273142)](https://www.abcam.cn/ps/products/273/ab273142/Images/ab273142-4-benefits-of-recombinant-antibodies.png)
Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (ab273142)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab273142 尚未被引用在任何文献中。
