Anti-p38IP抗体(ab122517)
Key features and details
- Rabbit polyclonal to p38IP
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-p38IP抗体
参阅全部 p38IP 一抗 -
描述
兔多克隆抗体to p38IP -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human p38IP aa 50-200.
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阳性对照
- IHC-P: Human testis tissue, cerebral cortex, prostate tissue, rectum tissue. ICC/IF: U-2 OS cells. WB: HEK-293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122517于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by Fgf signaling and Snail (By similarity). Required for starvation-induced ATG9A trafficking during autophagy. -
组织特异性
Highly expressed in testis, moderately in brain and pituitary gland. Expressed in several fetal tissues, including lung, brain, thymus and kidney. Expression is down-regulated in malignant prostate tissues. -
序列相似性
Belongs to the FAM48 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 55578 Human
- Omim: 613417 Human
- SwissProt: Q8NEM7 Human
- Unigene: 435815 Human
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别名
- AA667204 antibody
- AI450544 antibody
- bA421P11.4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (ab122517)
Paraffin embedded human testis tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-p38IP antibody (ab122517)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli. Recommended concentration of ab122517 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Western blot - Anti-p38IP antibody (ab122517)Lanes 2-3 : Anti-p38IP antibody (ab122517)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing p38IP with a C-terminal myc-DDK tag (~3.1kDa) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (ab122517)Paraffin embedded human prostate tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (ab122517)Paraffin embedded human cerebral cortex tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (ab122517)Paraffin embedded human rectum tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab122517 被引用在 2 文献中.
- Han J et al. Sesquiterpene lactones-enriched fractions from Xanthium mongolicum Kitag alleviate RA by regulating M1 macrophage polarization via NF-κB and MAPK signaling pathway. Front Pharmacol 14:1104153 (2023). PubMed: 36778009
- Zhao S et al. Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells. J Immunother Cancer 10:N/A (2022). PubMed: 35768165