Key features and details
- Mouse monoclonal [M139] to p38 (phospho T180 + Y182)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG1
产品名称Anti-p38 (phospho T180 + Y182)抗体[M139]
参阅全部 p38 一抗
描述小鼠单克隆抗体[M139] to p38 (phospho T180 + Y182)
特异性This antibody detects a 42 kDa protein corresponding to the apparent molecular mass of p38a. This peptide sequence is highly conserved in the p38ß,g, and d MAPKs, and is identical in human and mouse p38a.
经测试应用适用于: WBmore details
种属反应性与反应: Mouse, Human
Synthetic peptide corresponding to Rat p38 (phospho T180 + Y182) conjugated to Keyhole Limpet Haemocyanin (KLH). Clone M139 was generated from a phospho-p38α (Thr-180/Tyr-182) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 180 and tyrosine 182 of rat p38α.
- Pervanadate treated human Jurkat and A431 cells and anisomycin treated human HeLa cells.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
纯度Protein A purified
纯化说明Mouse monoclonal purified with protein A chromatography.
功能Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
组织特异性Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
翻译后修饰Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
Serum starved HepG2 cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of (±)-naringenin (ab120958). Increased expression of p38 (phospho T180 + Y182) (
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with
μg /ml and ab8227 at 1/1000 dilution overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 and visualised using ECL development solution.
All lanes : Anti-p38 (phospho T180 + Y182) antibody [M139] (ab45381) at 1/1000 dilution
Lane 1 : A431 cells serum starved overnight
Lane 2 : A431 cells treated with pervanadate (1 mM) for 30 minutes
Lane 3 : A431 cells treated with pervanadate (1 mM) for 30 minutes with No peptide
Lane 4 : A431 cells treated with pervanadate (1 mM) for 30 minutes with phospho-ERK1 (T202/Y204) peptide
Lane 5 : A431 cells treated with pervanadate (1 mM) for 30 minutes with phoshpo-p38 (T180/Y182) peptide
Lysates/proteins at 20 µg per lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
ab45381 被引用在 31 文献中.
- Sun B et al. Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta. J Orthop Translat 38:175-189 (2023). PubMed: 36439629
- Chen Y et al. PP1A prevents ROS-induced pyroptosis by inhibiting MAPK/caspase-3 in mouse adipose tissue. FEBS J 289:3839-3853 (2022). PubMed: 35080339
- Nikooie R et al. Lactate regulates autophagy through ROS-mediated activation of ERK1/2/m-TOR/p-70S6K pathway in skeletal muscle. J Cell Commun Signal 15:107-123 (2021). PubMed: 33398722
- Jing J et al. Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells. Cell Death Dis 11:626 (2020). PubMed: 32796811
- Dai F et al. Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma. Aging (Albany NY) 12:14037-14049 (2020). PubMed: 32667904