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Anti-p35抗体(ab64960)

Submit a review Q&A (2)References (2)
Western blot - Anti-p35 antibody (ab64960)
  • Immunocytochemistry/ Immunofluorescence - Anti-p35 antibody (ab64960)

Key features and details

  • Rabbit polyclonal to p35
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-p35抗体
    参阅全部 p35 一抗

  • 描述

    兔多克隆抗体to p35

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Rat, Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide (Human) from an internal sequence

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab64960于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
ICC/IF
1/500 - 1/1000.
说明
WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
ICC/IF
1/500 - 1/1000.

靶标

  • 功能

    p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.

  • 组织特异性

    Brain and neuron specific.

  • 序列相似性

    Belongs to the cyclin-dependent kinase 5 activator family.

  • 翻译后修饰

    The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.

  • 细胞定位

    Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CD5R1_HUMAN antibody
    • CDK5 activator 1 antibody
    • CDK5P35 antibody
    • CDK5R antibody
    • CDK5R1 antibody
    • Cyclin dependent kinase 5 regulatory subunit 1 antibody
    • Cyclin-dependent kinase 5 activator 1 antibody
    • Cyclin-dependent kinase 5 regulatory subunit 1 antibody
    • NCK 5A antibody
    • Neuronal CDK5 activator antibody
    • p23 antibody
    • p25 antibody
    • p35 antibody
    • Regulatory partner for CDK5 kinase antibody
    • Tau protein kinase II 23 kDa subunit antibody
    • TPKII regulatory subunit antibody
    see all

图片

  • Western blot - Anti-p35 antibody (ab64960)
    Western blot - Anti-p35 antibody (ab64960)
    All lanes : Anti-p35 antibody (ab64960) at 1/500 dilution

    Lane 1 : Rat brain cell extract
    Lane 2 : Rat brain cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 34 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-p35 antibody (ab64960)
    Immunocytochemistry/ Immunofluorescence - Anti-p35 antibody (ab64960)
    Immunofluorescence analysis of HeLa cells, using ab64960 at 1/500 dilution. Right hand panel was also treated with immunising peptide.

文献 (2)

发表研究结果有使用 ab64960？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab64960 被引用在 2 文献中.

  • Wang H  et al. Involvement of the miR-137-3p/CAPN-2 Interaction in Ischemia-Reperfusion-Induced Neuronal Apoptosis through Modulation of p35 Cleavage and Subsequent Caspase-8 Overactivation. Oxid Med Cell Longev 2020:2616871 (2020). PubMed: 33456665
  • Bai X  et al. CDK5 promotes renal tubulointerstitial fibrosis in diabetic nephropathy via ERK1/2/PPAR? pathway. Oncotarget 7:36510-36528 (2016). PubMed: 27145370

客户评价及客户问答

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Question

I ran the Hela/Rat brain blots today and will try various probing conditions tomorrow. If these are positive, I can send you the figures for your own records. Hopefully this will tell us something about the endogenous levels of p35 in HEK cells too… Thanks for your help!

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Answer

Thank you for your reply. I look forward to seeing your results from the current western blot. Please let me know if there is anything else I can

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Question

Thank you for your reply. The catalogue antibody is ab64960 Please let me know if you have some suggestions, Best wishes, Tania

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Answer

Thank you for providing the catalogue number of the antibody. The ab64960, anti-p35 antibody, is guaranteed to work in western blot and so is covered under our Abpromise, which is a guarantee that covers our antibodies for 6 months to work in the applications and species as stated on the data sheet. I was wondering if HEK-293 cells actually express p35, I ask as one of our other p35 antibodies, ab66064, was tested in transfecetd vs non-transfected HEK-293T cells, and no signal for p35 was detected in the non-transfected cells. Have you tried to run HEK-293 cell lysate samples and either HeLa cell or rat brain lysates and see if you get a signal from any of these samples? Please let me know if you have tried any of the tips from the previous email, or whether you have any information as to whether p35 is endogenously expressed in HEK293 cells. If there is anything else I can help with, please do not hesitate to contact

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