Anti-P2Y2抗体(ab272891)
Key features and details
- Rabbit polyclonal to P2Y2
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-P2Y2抗体
参阅全部 P2Y2 一抗 -
描述
兔多克隆抗体to P2Y2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human P2Y2. The exact sequence is proprietary. Center region.
Database link: P41231 -
阳性对照
- WB: HEK-293T and A431 whole cell lysate. Mouse and rat brain tissue lysate. ICC/IF: DIV14 rat E18 primary cortical neurons cells. IP: HEK-293T whole cell extracts.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272891于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
|
| IP |
1/100 - 1/500.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
ICC/IF
1/100 - 1/1000. |
|
IP
1/100 - 1/500. |
靶标
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功能
Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. The affinity range is UTP = ATP > ATP-gamma-S >> 2-methylthio-ATP = ADP. -
组织特异性
Spleen, testis, kidney, liver, lung, heart and brain. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5029 Human
- Entrez Gene: 18442 Mouse
- Entrez Gene: 29597 Rat
- Omim: 600041 Human
- SwissProt: P41231 Human
- SwissProt: P35383 Mouse
- SwissProt: P41232 Rat
- Unigene: 339 Human
see all -
别名
- ATP receptor antibody
- HP2U antibody
- Human P2U nucleotide receptor mRNA complete cds antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-P2Y2 antibody (ab272891)
4% PFA-fixed DIV14 rat E18 primary cortical neurons cells stained for P2Y2 using ab272891 at 1/500 (green) dilution in ICC/IF.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500.
Blue: Fluoroshield with DAPI.
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Western blot - Anti-P2Y2 antibody (ab272891)All lanes : Anti-P2Y2 antibody (ab272891) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.10% SDS PAGE
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Immunoprecipitation - Anti-P2Y2 antibody (ab272891)Immunoprecipitation of P2Y2 protein from HEK-293T whole cell extracts using 5 μg of ab272891.
Lane 1: HEK-293T whole cell;
Lane 2: Control IgG;
Lane 3: Immunoprecipitate.
Western blot analysis was performed using ab272891, followed by anti-Rabbit IgG-HRP as a secondary reagent.
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Western blot - Anti-P2Y2 antibody (ab272891)All lanes : Anti-P2Y2 antibody (ab272891) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 50 µg per lane.10% SDS-PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272891 尚未被引用在任何文献中。