重组Anti-P2X7抗体[EPR24130-77]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] (AB259942)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling P2X7 with ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse cerebrum (PMID : 28716092).The section was incubated with ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] (AB259942)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling P2X7 with ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining in mouse spleen (PMID : 22415881).The section was incubated with ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] (AB259942)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling P2X7 with ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Very weak staining in mouse skeletal muscle.The section was incubated with ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] (AB259942)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling P2X7 with ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining in sinusiod of mouse liver (PMID : 22415881).The section was incubated with ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-P2X7 antibody [EPR24130-77] (AB259942)

P2X7 was immunoprecipitated from 0.35 mg Mouse lung tissue lysate 10 ug with ab259942 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259942 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse lung tissue lysate 10 ug

Lane 2 : ab259942 IP in Mouse lung tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259942 in Mouse lung tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3.25 seconds

All lanes:

Immunoprecipitation - Anti-P2X7 antibody [EPR24130-77] (ab259942)

Predicted band size: 69 kDa

Observed band size: 75 kDa

false

• WB

Lab

Western blot - Anti-P2X7 antibody [EPR24130-77] (AB259942)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Samples are non-boiled as boiling may cause protein aggregates.

The molecular weight observed is consistent with what has been described in the literature (PMID : 20450227)

The expression profile is consistent with what has been described in the literature (PMID : 29018292)

Low expression or negative control : Mouse skeletal muscle (PMID : 15254086, PMID : 22415881), which is consistent to IHC expression pattern.

Exposure time : 59 seconds

All lanes:

Western blot - Anti-P2X7 antibody [EPR24130-77] (ab259942) at 1/1000 dilution

Lane 1:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

Lanes 1 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 75 kDa

false